Abstract
Activity levels of cytokines were measured by stimulation of the cell lines NFS-60, 7TD1, and TF-1. In 39 samples of amniotic fluid, levels of Granulocyte-Stimulating Factor (G-CSF) were 1434±2063 (mean±SD) and of Interleukin (IL-6) 546±1071 pg/ml; Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was not detectable. IL-6 was correlated to G-CSF (r=0.3; p=0.003). G-CSF (p=0.0002) and IL-6 (p=0.006) were influenced by Alpha-Fetoprotein (AFP) and G-CSF by rhesus-incompatibility (p=0.0004). These findings suggest that cytokines such as IL-6 and G-CSF play some role in physiological and pathological pregnancy.
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Abbreviations
- G-CSF:
-
granulocyte-colony stimulating factor
- GM-CSF:
-
granulocyte-macrophage colony stimulating factor
- M-CSF:
-
macrophage-colony stimulating factor
- CSF:
-
colonystimulating factor
- IL-6:
-
interleukin-6
- IL-11:
-
interleukin-11
- AFP:
-
alpha-fetoprotein
- NFS-60:
-
cell line
- 7TD1:
-
cell line
- TF-l:
-
cell line
- rh:
-
recombinant human
- MTT:
-
3-(4,5-dimethylthiazol2-yl)-2,5-diphenyl tetrazolium bromide
- FCS:
-
fetal calf serum
- RPMI 1640:
-
nutrient solution
- ATCC:
-
American Tissue Culture Collection
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Weimann, E., Reisbach, G., Reinsberg, J. et al. IL-6 and G-CSF levels in amniotic fluid during the second trimester in normal and abnormal pregnancies. Arch Gynecol Obstet 256, 125–130 (1995). https://doi.org/10.1007/BF01314640
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DOI: https://doi.org/10.1007/BF01314640