Summary
Recombinant DNA techniques were used to insert foreign genes into bovine herpesvirus-1 [infectious bovine rhinotracheitis virus (IBRV)] vectors which were attenuated by deletion and/or insertion mutations in the IBRV thymidine kinase (tk) gene. In one recombinant, the regulatory and coding sequences of the late pseudorabies virus (PRV) glycoprotein gIII gene, were inserted into the early IBRV tk gene. This recombinant efficiently expressed the PRV gIII gene indicating that immediate early IBRV proteins were competent to transactivate the late PRV gIII gene. IBRV vector viruses were also prepared in which the coding sequences of the early PRV tk gene, the late PRV gIII gene, and theE. coli β-galactosidase gene were ligated to the late IBRV gIII promoter. Genotypes and phenotypes of the recombinant viruses were verified by restriction endonuclease and molecular hybridization experiments, thymidine plaque autoradiography, β-gal plaque assays, and by immunoprecipitation experiments on extracts from3H-mannose-labelled cells. The recombinant IBRV expressing β-gal from the IBRV gIII promoter has been useful as an intermediate in the construction of IBRV vectors harboring foreign DNA sequences. The infectivity of the IBRV recombinant that expressed PRV gIII from the IBRV gIII promoter, was neutralized by polyclonal PRV antisera and by monoclonal antibodies to PRV gIII. The PRV gIII glycoprotein synthesized by the preceding recombinant has been used to coat microtiter test plate wells in a PRV gIII differential diagnostic test kit.
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Cai W, Schaffer PA (1989) Herpes simplex virus type 1 ICP0 plays a critical role in the de novo synthesis of infectious virus following transfection of viral DNA. J Virol 63: 4579–4589
Casadaban MJ, Martinez-Arias A, Shapira SK, Chou J (1983) β-galactosidase gene fusion for analyzing gene expression inEscherichia coli and yeast. Methods Enzymol 100: 293–308
Cook CG, Letchworth GJ, Splitter GA (1989) Bovine naturally cytolytic cell activation against bovine herpes virus type 1-infected cells does not require late viral glycoproteins. Immunology 66: 565–569
Davison AJ, Moss B (1989) Structure of vaccinia virus early promoters. J Mol Biol 210: 749–769
Enquist LW, Keeler Jr CL, Robbins AK, Ryan JP, Whealy ME (1988) An aminoterminal deletion mutation of pseudorabies virus glycoprotein gIII affects protein localization and RNA accumulation. J Virol 62: 3565–3573
Gait MJ (ed) (1984) Oligonucleotide syntheses: a practical approach. IRL Press, Washington, DC
Inchauspe G, Ostrove JM (1989) Differential regulation by varicella-zoster virus (VZV) and herpes simplex virus type-1 trans-activating genes. Virology 173: 710–714
Kit M, Kit S (1987) US Patent No. 4,703,001
Kit M, Kit S (1991) Sensitive glycoprotein gIII blocking ELISA to distinguish between pseudorabies (Aujeszky's disease)-infected and vaccinated pigs. Vet Microbiol 28: 141–155
Kit M, Kit S, Little SP, DiMarchi RD, Gale C (1991) Bovine herpesvirus-1 (infectious bovine rhinotracheitis virus)-based viral vector which expresses foot and mouth disease epitopes. Vaccine 9: 564–572
Kit S (1988) Genetically engineered pseudorabies and infectious bovine rhinotracheitis virus vaccines. In: Lasky L (ed) Technological advances in vaccine development. Alan R Liss, New York, pp 183–195 (UCLA symposium on molecular and cellular biology, vol 84)
Kit S, Kit M (1991) Genetically engineered herpesvirus vaccines. In: Melnick JL (ed) Progress in medical virology. Karger, Basel, pp 128–166
Kit S, Kit M, DiMarchi RD, Little SP, Gale C (1991) Modified-live infectious bovine rhinotracheitis virus vaccine expressing monomer and dimer forms of foot and mouth disease capsid protein epitopes on surface of hybrid virus particles. Arch Virol 120: 1–17
Kit S, Kit M, McConnell S (1986) Intramuscular and intravaginal vaccination of pregnant cows with thymidine kinase-negative, temperature-resistant infectious bovine rhinotracheitis virus (bovine herpesvirus 1). Vaccine 4: 55–61
Kit S, Kit M, Pirtle EC (1985) Attenuated properties of thymidine kinase-negative deletion mutant of pseudorabies virus. Am J Vet Res 46: 1359–1367
Kit S, Otsuka H, Kit M (1990) Gene-deleted IBRV marker vaccine. Vet Rec 127: 363–364
Kit S, Qavi H, Gaines JD, Billingsley P, McConnell S (1985) Thymidine kinase-negative bovine herpesvirus type 1 mutant is stable and highly attenuated in calves. Arch Virol 86: 63–83
Kit S, Sheppard M, Ichimura H, Kit M (1987) Second generation pseudorabies vaccine with deletions in thymidine kinase and glycoprotein genes. Am J Vet Res 48: 780–793
Kozmik Z, Arnold L, Paces V (1991) Multible sets of adjacent μE1 and oct-1 binding sites upstream of the pseudorabies virus immediate-early gene promoter. Virology 182: 239–249
Levine M, Krikos A, Glorioso JC, Homa FL (1990) Regulation of expression of the glycoprotein genes of herpes simplex virus type 1 (HSV-1). In: Lopez C et al (eds) Immunobiology and prophylaxis of human herpesvirus infections. Plenum Press, New York, pp 151–163
Mavromara-Nazos P, Roizman B (1987) Activation of herpes simplex virus gamma 2 genes by viral DNA replication. Virology 161: 593–598
Mettenleiter TC, Rauh I (1990) A glycoprotein gX-β-galactosidase fusion gene as insertional marker for rapid identification of pseudorabies virus mutants. J Virol Methods 30: 55–66
Nelson R, Adachi AM, Chisholm H, Misra V (1989) Temperature-sensitive mutants of bovine herpesvirus type 1: mutants which make unaltered levels of “early” glycoproteins but fail to synthesize a “late” glycoprotein. J Gen Virol 70: 125–132
Otsuka H, Kit S (1984) Nucleotide sequence of the marmoset herpesvirus thymidine kinase gene and predicted amino acid sequence of thymidine kinase polypeptide. Virology 135: 316–330
Rauh I, Weiland F, Fehler F, Keil GM, Mettenleiter TC (1991) Pseudorabies virus mutants lacking the essential glycoprotein gII can be complemented by glycoprotein gI of bovine herpesvirus 1. J Virol 65: 621–631
Robbins AK, Ryan JP, Whealy ME, Enquist LW (1989) The gene encoding the gIII envelope protein of pseudorabies virus vaccine strain Bartha contains a mutation affecting protein localization. J Virol 63: 250–258
Sheppard M, Otsuka H, Kit M, Kit S (1986) Transcription and in vitro translation of pseudorabies virus (PRV) thymidine kinase (TK) gene. In: Abstracts 86th annual meeting of the American Society of Microbiology, Washington, DC, p 311.65: 621–631
Su L, Knipe DM (1989) Herpes simplex virus alpha protein ICP27 can inhibit or augment viral gene transactivation. Virology 170: 496–504
Thomsen DR, Marotti KR, Palermo DP, Post LE (1987) Pseudorabies virus as a live virus vector for expression of foreign genes. Gene 57: 261–265
van Zijl M, Wensvoort G, de Kluyver E, Hulst M, van der Gulden H, Gielkens A, Berns A, Moormann R (1991) Live attenuated pseudorabies virus expressing envelope glycoprotein E1 of hog cholera virus protects swine against both pseudorabies and hog cholera. J Virol 65: 2761–2765
Vlcek C, Kozmik Z, Paces V, Schirm S, Schwyzer M (1990) Pseudorabies virus immediate-early gene overlaps with an oppositely oriented open reading frame: characterization of their promoter and enhancer regions. Virology 179: 365–377
Whealy ME, Baumeister K, Robbins AK, Enquist LW (1988) A herpesvirus vector for expression of glycosylated membrane antigens: fusion proteins of pseudorabies virus gIII and human immunodeficiency virus type 1 envelope glycoproteins. J Virol 62: 4185–4194
Whealy ME, Robbins AK, Enquist LW (1989) Replacement of the pseudorabies virus glycoprotein gIII gene with its postulated homolog, the glycoprotein gC gene of herpes simplex virus type 1. J Virol 63: 4055–4059
Wirth UV, Gunkel K, Engels M, Schwyzer M (1989) Spatial and temporal distribution of bovine herpesvirus 1 transcripts. J Virol 63: 4882–4889
Wirth UV, Vogt B, Schwyzer M (1991) The three major immediate-early transcripts of bovine herpesvirus 1 arise from two divergent and spliced transcription units. J Virol 65: 195–205
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Kit, S., Otsuka, H. & Kit, M. Expression of porcine pseudorabies virus genes by a bovine herpesvirus-1 (infectious bovine rhinotracheitis virus) vector. Archives of Virology 124, 1–20 (1992). https://doi.org/10.1007/BF01314621
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DOI: https://doi.org/10.1007/BF01314621