Skip to main content
Log in

Effect of the infection of rubella virus on BHK 21/13 S cells: Study of a glycolipid

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

A unique late effect of rubella virus infection of BHK21/13S cells, was the formation of a glycolipid compound detected neither in control cells nor in virus. The glycolipid was shodied mainly by gas-liquid chromatography and found to contain fucose, glycerol with a molar ratio 2:1 and palmitic, stearic, oleic and myristic acids.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Anderson, R., Dales, S.: Biogenesis of poxviruses: glycolipid metabolism in vaccinia-infected cells. Virology84, 108–117 (1978).

    Google Scholar 

  2. Anderson, R.: Regulation of glycolipid biosynthesis: effects of virus infection and drug-induced translational inhibition on glycolipid metabolism. Biochemistry18, 2395–2400 (1979).

    Google Scholar 

  3. Bardeletti, G., Kessler, N., Aymard-Henry, M.: Morphology, biochemical analysis and neuraminidase activity of rubella virus. Arch. Virol.49, 175–186 (1975).

    Google Scholar 

  4. Bardeletti, G., Gautheron, D. C.: Phospholipid and cholesterol composition of rubella virus and its host cell BHK21 grown in suspension cultures. Arch. Virol.52, 19–27 (1976).

    Google Scholar 

  5. Bardeletti, G.: Respiration and ATP level in BHK21/13S cells during the earliest stages of Rubella Virus replication. Intervirology8, 100–109 (1977).

    Google Scholar 

  6. Bardeletti, G., Tektoff, J., Gautheron, D.: Rubella virus maturation and production in two host cell systems. Intervirology11, 97–103 (1979).

    Google Scholar 

  7. Huang, R. T. C.: Sphingolipids of influenza virus. Biochem. Biophys. Acta424, 90–97 (1976).

    Google Scholar 

  8. Aktes, M.: In:Works, T. S. and (eds.), Techniques of Lipidology, 394. New York: American Elsevier Publishing Co. 1972.

    Google Scholar 

  9. Katow, S., Matsuno, T.: Base composition of rubella virus RNA. Arch. Virol.65, 67–70 (1980).

    Google Scholar 

  10. Ruhlig, M. A., Person, S.: Alterations of neutral glycolipids in cells infected with syncytium producing mutants of herpes simplex virus type 1. J. Virol.24, 602–608 (1977).

    Google Scholar 

  11. Schachter, H.: The subcellular sites of glycosylation. Biochem. Soc. Symp.40, 57–71 (1974).

    Google Scholar 

  12. Schlenk, H., Gellerman, J. L.: Esterification of fatty acids with diazomethane on a small scale. Anal. Chem.32, 1412–1414 (1960).

    Google Scholar 

  13. Vaheri, A., Cristofalo, V. J.: Metabolism of rubella virus infected BHK21 cells. Arch. ges. Virusforsch.21, 425–436 (1967).

    Google Scholar 

  14. Voiland, A., Bardeletti, G.: Fatty acid composition of rubella virus and BHK21/13S infected cells. Arch. Virol.64, 319–328 (1980).

    Google Scholar 

  15. von Bonsdorff, C. H., Vaheri, A.: Growth of rubella virus in BHK21 cells electron microscopy of morphogenesis. J. gen. Virol.5, 47–51 (1969).

    Google Scholar 

  16. Warren, L.: The thiobarbituric assays of sialic acids. J. biol. Chem.234, 1971–1975 (1959).

    Google Scholar 

  17. Wechsler, S. L., Fields, B. N.: Intracellular synthesis of measles virus specified polypeptides. J. Virol.25, 285–297 (1978).

    Google Scholar 

  18. Ziminsky, T., Borowsky, E.: A new spray reagent replacing sulphuric acid in thin layer chromatography. J. Chromatog.23, 480–482 (1966).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

With 1 Figure

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bardeletti, G., Voiland, A. Effect of the infection of rubella virus on BHK 21/13 S cells: Study of a glycolipid. Archives of Virology 68, 285–289 (1981). https://doi.org/10.1007/BF01314583

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01314583

Keywords

Navigation