Skip to main content
Log in

Virus-specific and cell-specific RNA transcripts in influenza virus-infected cells: The rate of synthesis and the content in the nuclei

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

The pool of nuclear poly (A)-containing RNA in influenza virus-infected cells as measured with steady-state3H-uridine labelling is represented predominantly by cell-specific RNA: virus-specific sequences constitute less than 17 per cent at 3 and 5 hours post infection.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Aviv, H., Leder, Ph.: Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc. Nat. Acad. Sci. U.S.A.69, 1408–1418 (1972).

    Google Scholar 

  2. Barry, R. D.: The effects of actinomycin D and ultraviolet irradiation on the production of fowl plague virus. Virology24, 563–569 (1964).

    Google Scholar 

  3. Bantle, J. A., Maxwell, J. H., Hahn, W. E.: Specificity of oligo (dT)-cellulose chromatography in the isolation of polyadenylated RNA. Anal. Biochem.72, 413–427 (1976).

    Google Scholar 

  4. Bonner, W. M., Laskey, R. A.: A film detection method for tritium labelled proteins and nucleic acids in polyacrylamide gels. Eur. J. Biochem.46, 83–88 (1974).

    Google Scholar 

  5. Bouloy, M., Morgan, M. A., Shatkin, A. J. Krug, R. M.: Cap and internal nucleotides of reovirus mRNA primers are incorporated into influenza viral complementary RNA during transcriptionin vitro. J. Virol.32, 895–904 (1979).

    Google Scholar 

  6. Bouloy, M., Plotch, S. J., Krug, R. M.: Globin mRNAs are primers for the transcription of influenza viral RNAin vitro. Proc. Nat. Acad. Sci. U.S.A.75, 4886–4890 (1978).

    Google Scholar 

  7. Franklin, R.: Purification and properties of the replicative intermediate of the RNA bacteriophage R 17. Proc. Nat. Acad. Sci. U.S.A.12, 1504–1511 (1966).

    Google Scholar 

  8. Gafford, L. G., Randall, C. C.: Virus-specific RNA and DNA in nuclei of cells infected with Fowlpox virus. Virology69, 1–14 (1976).

    Google Scholar 

  9. Hay, A. J., Lomniczi, B., Bellamy, A. R., Skehel, J. J.: Transcription of the influenza virus genome. Virology83, 337–355 (1977).

    Google Scholar 

  10. Ito, Y., Joklik, W. K.: Temperature sensitive mutants of reovirus. I. Patterns of gene expression by mutants of groups C, C and E. Virology50, 189–201 (1979).

    Google Scholar 

  11. Kaverin, N. V., Varich, N. L.: Newcastle disease virus-specific RNA: polyacrylamide gel analysis of single-stranded RNA and hybrid duplexes. J. Virol.13, 253–260 (1974).

    Google Scholar 

  12. Kaverin, N. V., Varich, N. L., Smirnov, Y. A.: Sedimentation pattern of virus-specific RNA synthesized in Newcastle disease virus-infected cells treated with amino acid analogues. Arch. ges. Virusforsch.41, 191–198 (1973).

    Google Scholar 

  13. Krug, R. M., Broni, B. A., Bouloy, M.: Are the 5′-ends of influenza viral mRNAs synthesizedin vivo donated by host mRNAs? Cell18, 329–334 (1979).

    Google Scholar 

  14. Krug, R. M., Morgan, M. A., Shatkin, A. J.: Influenza virus mRNA contains internal N6-methyladenosine and 5′-terminal 7-methylguanosine in cap structures. J. Virol.20, 45–53 (1976).

    Google Scholar 

  15. Mahy, B. W. J., Hastie, N. D., Armstrong, S. J.: Inhibition of influenza virus replication by α-amanitine: mode of action. Proc. Nat. Acad. Sci. U.S.A.69, 1421–1424 (1972).

    Google Scholar 

  16. Petrik, Yu., Sklyanskaya, E. I., Varich, N. L.: Isolation of nuclei from influenza virus infected chick fibroblast cells. Vopr. Virusol.2, 157–161 (1980).

    Google Scholar 

  17. Plotch, S. J., Bouloy, M., Krug, R. M.: Transfer of the 5′-terminal cap of the globin mRNA to influenza viral complementary RNA during transcriptionin vitro. Proc. Nat. Acad. Sci.76, 1618–1623 (1979).

    Google Scholar 

  18. Robertson, H. D., Dickson, S., Plotch, S. J., Krug, R. M.: Identification of the RNA region transfered from a representative primer, ß-globin mRNA, to influenza mRNA duringin vitro transcription. Nucleic Acid Res.8, 925–943 (1980).

    Google Scholar 

  19. Rott, R., Saber, S., Scholtissek, C.: Effect on myxovirus of mitomycin C, actinomycin D and pretreatment of the host cell with ultra-violet light. Nature (London)205, 1187–1190 (1965).

    Google Scholar 

  20. Sklyanskaya, E. I., Rudneva, I. A., Vovk, T. S., Kaverin, N. V.: Processing of influenza HA protein in MDCK cells: components with different mobilities in polyacrylamide gel electrophoresis and their precursor-product relationships. Arch. Virol.65, 257–267 (1980).

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

With 2 Figures

Rights and permissions

Reprints and permissions

About this article

Cite this article

Varich, N.L., Petrík, Y., Farashyan, V.R. et al. Virus-specific and cell-specific RNA transcripts in influenza virus-infected cells: The rate of synthesis and the content in the nuclei. Archives of Virology 68, 279–284 (1981). https://doi.org/10.1007/BF01314582

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01314582

Keywords

Navigation