Summary
Kinetics of the UV-induced (λ=254 nm) inactivation of phage MS2 is studied for stirred and non-stirred solutions with optical densities ranging from 0.06 to 2.1. The extent of inactivation exhibits exponential dependence on irradiation time for stirred solutions thus indicating the homogeneity of the phage population in respect to photosensitivity of infectivity. Irradiation of the same solution without stirring results in deviation of survival curves from exponential ones (the so called “tailing”), being more pronounced the larger the optical density of the layer under irradiation and degree of inactivation. Such a deviation is conditioned by non-uniform light absorption by virions in solution in the absence of stirring. The inactivation kinetics upon UV-irradiation of the stirred and non-stirred phage solutions is rather accurately expressed by respective equations thus enabling the choice of rational conditions for a given depth of phage inactivation to be performed.
Similar content being viewed by others
References
Budowsky, E. I., Bresler, S. E., Freidman, E. A., Zheleznova, N. V.: Principles of selective inactivation of viral genome. I. UV-induced inactivation of influenza virus. Arch. Virol.68, 239–247 (1981).
Elad, D.: Photoproducts of purines. In:Wang, S. Y. (ed.), Photochemistry and Photobiology of Nucleic Acids, Vol. 1, 357. New York: Academic Press 1976.
Fisher, C. J., Johns, H. E.: Pyrimidine photohydrates. In:Wang, S. Y. (ed.), Photochemistry and Photobiology of Nucleic Acids, Vol. 1, 162–225. New York: Academic Press 1976.
Fisher, C. J., Johns, H. E.: Pyrimidine photodimers. In:Wang, S. Y. (ed.), Photochemistry and Photobiology of Nucleic Acids, Vol. 1, 226–289. New York: Academic Press 1976.
Furuse, K., Watanabe, J.: Effect of ultraviolet light irradiation on RNA phage in H2O and in D2O. Virology46, 171–172 (1971).
Gavrilov, A. G., Menshonkova, T. N., Piskunkova, N. F., Pospelov, M. E., Fraikin, G. I., Rubin, L. B.: On the specificity of the effect of laser UV radiation on the survival of microorganisms. Dokl. Akad. Nauk S.S.S.R.239, 1238–1240 (1978). (In Russian.)
Gordon, A. J., Ford, R. A.: In: The Chemists Companion, 514. New York-London-Sydney-Toronto: J. Wiley 1972.
Kazimierczuk, Z., Giziewicz, J., Sugar, D.: Photochemical transformation of N1-methoxyadenine and N1-methoxy-9-methyladenine. Acta biochim. polon.20, 169–179 (1973).
Korn, G. A., Korn, T. M.: Mathematical handbook for scientists and engineers. New York-San Francisco-Toronto-London: McGraw-Hill 1968.
Kryukov, P. G., Letokhov, V. S., Nikogosyan, D. N., Borodavkin, A. V., Budowsky, E. I., Simukova, N. A.: Multiquantum photoreactions of nucleic acid components in aqueous solution by powerful ultraviolet picosecond radiation. Chem. Phys. Letters61, 375–379 (1979).
Menshonkova, T. N., Simukova, N. A., Budowsky, E. I., Rubin, L. B.: The effect of high intensity ultraviolet irradiation on nucleic acids and their components. Cleavage of N-glycosidic bond in thymidine, adenosine and 2′-deoxyadenosine. FEBS Letters112, 299–301 (1980).
Morowitz, H. J.: Absorption effects in volume irradiation of microorganisms. Science111, 229–230 (1950).
Remsen, J. F., Miller, N., Cerutti, P. A.: Photohydration of uridine in the RNA of coliphage R 17, II. The relationship between ultraviolet inactivation and uridine photohydration. Proc. Natl. Acad. Sci. U.S.A.65, 460–466 (1970).
Rosental, G. F., Jalynskaya, A. K., Markushevich, V. N., Bauman, V. R., Gren, E. Ja.: Preparative method of producting bacteriophage MS 2. Applied biochemistry and microbiology, Academy of Sciences of the U.S.S.R.7, 723–727 (1971). (In Russian.)
Simukova, N. A., Menshonkova, T. N., Budowsky, E. I., Rubin, L. B.: The action of high-intensity UV radiation on thymine. Dokl. Akad. Nauk S.S.S.R.250, 1398–1402 (1980). (In Russian.)
Smith, K. C.: The radiation-induced addition of proteins and other molecules to nucleic acids. In:Wang, S. Y. (ed.), Photochemistry and Photobiology of Nucleic Acids, Vol. 2, 187–218. New York: Academic Press 1976.
Wang, S. Y.: Pyrimidine biomolecular photoproducts. In:Wang, S. Y. (ed.), Photochemistry and Photobiology of Nucleic Acids, Vol. 1, 295–356. New York: Academic Press 1976.
Werbin, H., Valentine, R. C., Hidalgo-Salvatierra, O., McLaren, A. D.: Photobiology of RNA bacteriophages. II. UV-Irradiation off2; effect on extracellular stages of infection and on early replication. Photochem. Photobiol.7, 253–255 (1968).
Author information
Authors and Affiliations
Additional information
With 3 Figures
Rights and permissions
About this article
Cite this article
Budowsky, E.I., Kostyuk, G.V., Kost, A.A. et al. Principles of selective inactivation of viral genome. Archives of Virology 68, 249–256 (1981). https://doi.org/10.1007/BF01314578
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01314578