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Variables that may influence the alkaline sedimentation pattern of herpes simplex virus DNA

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Summary

Herpes simplex virus (HSV) DNA sediments homogeneously on a neutral sucrose gradient but heterogeneously on an alkaline sucrose gradient. Several factors that may influence the alkaline sedimentation pattern of HSV DNA were examined: e.g., the host cell, cell density at time of infection, multiplicity of infection, and the starting material for HSV DNA purification (whether HSV-infected cells or cell-free virus). Based on alkaline sedimentation analysis, these factors appear to play little or no role in the amount of intact single-stranded HSV DNA observed.

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Authors and Affiliations

  1. Department of Microbiology and Specialized Cancer Research Center, The Pennsylvania State University College of Medicine, Hershey, Pennsylvania, USA

    J. R. Ecker & R. W. Hyman

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  1. J. R. Ecker
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  2. R. W. Hyman
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Ecker, J.R., Hyman, R.W. Variables that may influence the alkaline sedimentation pattern of herpes simplex virus DNA. Archives of Virology 68, 221–228 (1981). https://doi.org/10.1007/BF01314575

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  • DOI: https://doi.org/10.1007/BF01314575

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