Summary
An isolate of canine parainfluenza (CPI) virus from the cerebrospinal fluid of a dog with neurological dysfunction was characterizedin vitro in comparison to a prototype strain of CPI virus, D008. The virus, designated 78–238 was found to be antigenically related to CPI virus (Manhatten strain) and simian virus 5 (SV5), but not to mumps virus (Enders strain). Ultrastructural observation of gradient-purified 78–238 virus revealed enveloped pleomorphic virions with helical nucleocapsid symmetry. Preliminary pathological studies indicated that 78–238 virus was encephalitogenic for gnotobiotic dogs when inoculated by the intracerebral route.
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Appel, M. J. G., Percy, D. H.: SV-5-like parainfluenza virus in dogs. J. Amer. vet. Med. Assoc.156, 1778–1781 (1970).
Appel, M. J. G., Gillespie, J. H.: Canine distemper virus. In: Virology Monographs, Vol. 11, 54–56. Wien-New York: Springer 1972.
Appel, M. J. G., Robson, D. S.: Microneutralization test for canine distemper virus. Amer. J. vet. Res.34, 1459–1463 (1973).
Armen, R. C., Evermann, J. F., Truant, A. L., Laughlin, C. A., Hallum, J. V.: Temperature sensitive mutants of measles virus produced from persistently infected HeLa cells. Arch. Virol.53, 121–132 (1977).
Binn, L. N., Eddy, G. A., Lazar, E. C., Helme, J., Murnane, T.: Viruses recovered from laboratory dogs with respiratory disease. Proc. Soc. exp. Biol. Med.126, 140–145 (1967).
Choppin, P. W., Stoeckenius, W.: The morphology of SV 5 virus. Virology23, 195–202 (1964).
Crandell, R. A., Brumlow, W. B., Davison, V. E.: Isolation of a parainfluenza virus from sentry dogs with upper respiratory disease. Amer. J. vet. Res.29, 2141–2157 (1968).
Evermann, J. F., Lincoln, J. D., McKeirnan, A. J.: Isolation of a paramyxovirus from the cerebrospinal fluid of a dog with posterior paresis. J. Amer. vet. Med. Assoc.177, 1132–1134 (1980).
Gibbs, E. L. J., Taylor, W. P., Lawman, M. J. P., Bryant, J.: Classification of Peste des petits ruminants virus as the fourth member of the genusMorbillivirus. Intervirol.11, 268–274 (1979).
Hsiung, G. D.: Parainfluenza-5 virus infection of man and animal. Prog. med. Virol.14, 241–274 (1972).
Kingsbury, D. W., Bratt, M. A., Choppin, P. W., Hanson, R. P.,et al: Paramyxoviridae. Intervirol.10, 137–152 (1978).
Krakowka, S., Long, D., Mezza, R., Mador, R. A., Koestner, A.: Derivation and maintenance of gnotobiotic dogs. Lab. Anim. Sci.28, 327–330 (1978).
Krakowka, S., Koestner, A.: Age-related susceptibility to infection with canine distemper virus in gnotobiotic dogs. J. inf. Dis.134, 629–632 (1976).
Lazar, E. C., Swango, L. J., Binn, L. N.: Serologic and infectivity studies of canine SV-5 virus. Proc. Soc. exp. Biol. Med.135, 173–176 (1970).
McCullough, B., Krakowka, S., Koestner, A., Shadduck, J.: Demyelinating activity of canine distemper virus isolates in gnotobiotic dogs. J. inf. Dis.130, 343–350 (1974).
Rosenberg, F. J., Lief, F. S., Todd, J. D., Rief, J. S.: Studies of canine respiratory viruses. I. Experimental infection of dogs with an SV-5-like canine parainfluenza agent. Amer. J. Epidemiol.94, 147–164 (1971).
Stone, A. B., Fox, M. W.: Clinico-pathological studies of an encephalitic syndrome in dogs. J. Small Anim. Prac.8, 709–715 (1967).
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Evermann, J.F., Krakowka, S., McKeirnan, A.J. et al. Properties of an encephalitogenic canine parainfluenza virus. Archives of Virology 68, 165–172 (1981). https://doi.org/10.1007/BF01314569
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DOI: https://doi.org/10.1007/BF01314569