Summary
Semliki Forest Virus (SFV) infected chick embryo cells (CEC) incubated in the presence of Tunicamycin, a drug which specifically inhibits glycosylation, were studied under the electron microscope. No extracellular virions were produced although intracellular viral nucleocapsids in paracrystalline form were detected. Under these conditions the structures, referred to as type 1 and 2 cytopathic vacuoles (cpv-1 and cpv-2, respectively) as well as membraneous spherules at the plasma membrane could still be observed.
CEC productively infected and transformed by Rous sarcoma virus (RSV) incubated for 24 hours in the presence of Tunicamycin (1 µg/ml) still showed budding and extracellular particles. Intracellular nucleocapsid structures could not be detected.
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References
Acheson, N. H., Tamm, I.: Replication of Semliki Forest Virus: An electron microscopic study. Virology32, 128–143 (1967).
de Guili, C., Kawai, S., Dales, S., Hanafusa, H.: Absence of surface projections on some noninfectious forms of RSV. Virology66, 253–260 (1975).
Grimley, P. M., Berezesky, I. K., Friedman, R. M.: Cytoplasmic structures associated with an arbovirus infection: Loci of viral ribonucleic acid synthesis. J. Virol.2, 1326–1338 (1968).
Grimley, P. M., Levin, J. G., Berezesky, I. K., Friedman, R. M.: Specific membraneous structures associated with the replication of group A arboviruses. J. Virol.10, 492–503 (1972).
Hanafusa, H., Miyamoto, T., Hanafusa, T.: A cell-associated factor essential for formation of an infectious form of Rous sarcoma virus. Proc. Natl. Acad. Sci. U.S.A.66, 314–321 (1970).
Kuo, S.-C., Lampen, J. O.: Tunicamycin—an inhibitor of yeast glycoprotein synthesis. Biochem. biophys. Res. Commun.58, 287–295 (1974).
Leavitt, R., Schlesinger, S., Kornfeld, S.: Tunicamycin inhibits glycosylation and multiplication of Sindbis and Vesicular stomatitis viruses. J. Virol.21, 375–385 (1977).
Mussgay, M., Rott, R.: Studies on the structure of a hemagglutinating component of a group A arbo virus (Sindbis). Virology23, 573–581 (1964).
Ogura, H., Bauer, H.: Biological and electron microscopic studies on the phenotypic mixing of the thermolabile mutant of Vesicular Stomatitis virus, tl-17, with avian RNA tumor viruses. Arch. Virol.52, 233–242 (1976).
Ogura, H., Friis, R.: Further evidence for the existence of a viral envelope protein defect in the Bryan high-titer strain of Rous sarcoma virus. J. Virol.16, 443–446 (1975).
Scheele, C. M., Hanafusa, H.: Proteins of helper-dependent RSV. Virology45, 401–410 (1971).
Schmidt, M. F. G., Schwarz, R. T., Ludwig, H.: Fluorosugars inhibit biological properties of different enveloped viruses. J. Virol.18, 819–823 (1976).
Scholtissek, C.: Inhibition of the multiplication of enveloped viruses by glucose derivatives. Curr. Top. Microbiol. Immunol.70, 101–119 (1975).
Schwarz, R. T., Rohrschneider, J. M., Schmidt, M. G. F.: Suppression of glycoprotein formation of Semliki Forest, influenza, and Avian sarcoma virus by Tunicamycin. J. Virol.19, 782–791 (1976).
Takatsuki, A., Arima, K., Tamura, G.: Tunicamycin, a new antibiotic. I. Isolation and characterization of Tunicamycin. J. Antibiot.24, 215–223 (1971).
Takatsuki, A., Tamura, G.: Tunicamycin, a new antibiotic. II. Some biological properties of the antiviral activity of Tunicamycin. J. Antibiot.24, 224–231 (1971).
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Ogura, H., Schmidt, M.F.G. & Schwarz, R.T. Effect of tunicamycin on the morphogenesis of Semliki Forest Virus and Rous sarcoma virus. Archives of Virology 55, 155–159 (1977). https://doi.org/10.1007/BF01314489
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DOI: https://doi.org/10.1007/BF01314489