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Caractérisation d'une polyédrose cytoplasmique chez le lépidoptèreEuxoa scandens, Riley (Noctuidae, Agrotinae). Etudesin vivo etin vitro

In vivo andin vitro studies of a cytoplasmic polyhedrosis virus isolated fromEuxoa scandens, Riley (Noctuidae, Agrotinae)

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Résumé

Les études sur un virus isolé à partir des larvesd'Euxoa scandens ont été faites d'une part en microscopie optique à l'aide de diverses colorations histologiques et histochimiques et d'autre part en microscopie électronique à partir de suspensions virales ou de tissus de larves infectées. Nos travaux montrent que ce virus de forme parasphérique et mesurant environ 70 nm appartient au groupe des polyédries cytoplasmiques. L'infectionin vivo se localise uniquement aux cellules de l'intestin moyen de la larve et se caractérise par l'inclusion des virus dans des corps protéiniques en très grande majorité de forme parasphérique. Les étapes de la pathogénèse virale ont été d'autre part étudiéesin vitro. L'infection des cultures cellulaires deLymantria dispar L., à partir de virus libres ou extraits par dissolution de polyèdres, a été réussie. Le stroma virogène et les premiers polyèdres très petits sont détectés dans le cytoplasme respectivement après 18 et 36 heures d'infection. Les corps d'inclusion exclusivement de forme cubique ont atteint leur taille maximum après 48 heures d'infection et sont présents nombreux dans le cytoplasme de plus de 90 per cent des cellules.

Summary

A virus isolated from the larvaes ofEuxoa scandens (Noctuidae, Agrotinae) was studied by histological and histochemical techniques, and by electron microscopic observation of virus suspensions and ultrathin sections of infected tissues. Our studies demonstrated that the virus in icosahedral in shape, measures 70 nm and belongs to the cytoplasmic polyhedrosis virus group. Only the midgut cells of the larvae were found to be infected and the virus particles were localized in inclusion bodies most of which had a paraspherical, polyhedral shape. It was possible to infectLymantria dispar L. cellsin vitro with free virus or with extracts obtained from the diseased larvae. The virogenic stroma and the first small inclusion bodies in this stroma were detected in the cytoplasm at 18 and 36 hours post infection, respectively. These polyhedra had a cubical shape, reached their maximum size after 48 hours and were located in the cytoplasm of over 90 per cent of the cells.

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  1. J. M. Quiot

    Present address: Station de recherches cytopathologiques, 30380, St. Christol les Alès, France

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  1. Département de Chimie-Biologie, Université du Québec à Trois-Rivières, Québec, Canada

    J. M. Quiot & S. Belloncik

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  1. J. M. Quiot
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  2. S. Belloncik
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Quiot, J.M., Belloncik, S. Caractérisation d'une polyédrose cytoplasmique chez le lépidoptèreEuxoa scandens, Riley (Noctuidae, Agrotinae). Etudesin vivo etin vitro . Archives of Virology 55, 145–153 (1977). https://doi.org/10.1007/BF01314488

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  • DOI: https://doi.org/10.1007/BF01314488

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