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Location of antigens associated with bovine leukemia virus

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Summary

Antigens associated with bovine leukemia virus (BLV) infection (gp 44/55 and p23) were identified by neutralization and immunofluorescent antibody (FA) tests. Only serums containing antibody to gp 45/55 neutralized the virus; antibody to p23 did not. These results indicate that gp 45/55 may be located in the virus envelope. In indirect FA tests, both a cytoplasmic fluorescence and a membrane fluorescence were detected in BLV-infected cells. The serum used in the FA test was then absorbed with either p23 or gp 45/55. The results of these absorption tests indicate that the membrane fluorescence maybe associated with gp 45/55 and the cytoplasmic fluorescence may be associated with p23.

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References

  1. Driscoll, D. M., Onuma, M., Olson, C.: Inhibition of Bovine Leukemia Virus Release by Antiviral Antibodies. Arch. Virol.55, 139–144 (1977).

    Google Scholar 

  2. Ferrer, J. F., Abt, D. A., Bhatt, D. M., Marshak, R. R.: Studies on the relationship between infection with bovine C-type virus, leukemia, and persistent lymphocytosis in cattle. Cancer Res.34, 893–900 (1974).

    Google Scholar 

  3. Ferrer, J. F., Avila, L., Stock, N. D.: Serological detection of type-C viruses found in bovine cultures. Cancer Res.32, 1864–1870 (1972).

    Google Scholar 

  4. Gilden, R. V., Long, C. W., Hanson, M., Toni, R., Charman, H. P., Oroszlan, S., Miller, J. M., Van Der Maaten, M. J.: Characteristics of the major internal protein and RNA polymerase of bovine leukemia virus. J. gen. Virol.29, 305–314 (1975).

    Google Scholar 

  5. Ikeda, H., Pincus, T., Yoshiki, T., Strand, M., August, J. T., Boyse, E. A., Mellors, R. C.: Biological expression of antigenic determinants of murine leukemia virus proteins gp 69/71 and p 30. J. Virol.14, 1274–1280 (1974).

    Google Scholar 

  6. McDonald, H. C., Graves, D. C., Ferrer, J. F.: Isolation and characterization of an antigen of the bovine C-type virus. Cancer Res.36, 1251–1257 (1976).

    Google Scholar 

  7. Miller, J. M., Olson, C.: Precipitating antibody to an internal antigen of the C-type virus associated with bovine lymphosarcoma. J. Natl. Cancer Inst.49, 1459 to 1462 (1972).

    Google Scholar 

  8. Miller, J. M., Van Der Maaten, M. J.: A complement fixation test for the bovine leukemia (C-type) virus. J. Natl. Cancer Inst.53, 1699–1702 (1974).

    Google Scholar 

  9. Onuma, M., Baumgartener, L. E., Olson, C., Pearson, L. D.: Fetal infection with bovine leukemia virus in sheep. (Submitted for publication.)

  10. Onuma, M., Mikami, T., Hayashi, T. T. A., Okada, K., Fujimoto, Y.: Studies of Marek's disease herpesvirus and turkey herpesvirus specific common antigen which stimulates the production of neutralizing antibodies. Arch. Virol.48, 85–97 (1975).

    Google Scholar 

  11. Onuma, M., Mikami, T., Hayashi, T. T. A.: Relation between common antigen and membrane antigens associated with Marek's disease herpesvirus and turkey herpesvirus infections. Arch. Virol.50, 305–309 (1976).

    Google Scholar 

  12. Onuma, M., Olson, C.: Tumor-associated antigen in bovine and ovine lymphosarcoma. Cancer Res. (in press).

  13. Onuma, M., Olson, C., Baumgartener, L. E.: Inhibition of bovine leukemia virus release. J. Natl. Cancer Inst.54, 1199–1202 (1975).

    Google Scholar 

  14. Onuma, M., Olson, C., Baumgartener, L. E., Pearson, L. D.: An ether-sensitive antigen associated with bovine leukemia virus infection. J. Natl. Cancer Inst.55, 1155–1158 (1975).

    Google Scholar 

  15. Onuma, M., Olson, C., Driscoll, D. M.: Properties of two isolated antigens associated with bovine leukemia virus infection. J. Natl. Cancer Inst.57, 571–578 (1976).

    Google Scholar 

  16. Paulsen, J., Rohde, W., Pauli, G., Harms, E., Bauer, H.: Comparative studies on ovine and bovine C-type particles. Bibl. Haemat.43, 190–192 (1976).

    Google Scholar 

  17. Pearson, G., Dewey, F., Klein, G., Henle, W.: Relation between neutralization of Epstein-Barr virus and antibodies to cell-membrane antigen induced by the virus. J. Natl. Cancer Inst.45, 989–995 (1970).

    Google Scholar 

  18. Ressang, A. A., Mastenbroek, N., Quak, J., Van Griensven, L. J. L. D., Calafat, J., Hilgers, J., Hageman, P. C., Souissi, T., Swen, S.: Studies on bovine leukemia. I. Establishment of type-C virus producing cell lines. Zbl. Vet. Med. B.21, 602–617 (1974).

    Google Scholar 

  19. Steeves, R. A., Strand, M., August, J. T.: Structural proteins of mammalian oncogenic RNA viruses. Murine leukemia virus neutralization by antisera prepared against purified envelope glycoprotein. J. Virol.14, 187–189 (1974).

    Google Scholar 

  20. Van Der Maaten, M. J., Miller, J. M., Boothe, A. D.: Replicating type-C virus particles in monolayer cell cultures of tissues from cattle with lymphosarcoma. J. Natl. Cancer Inst.52, 491–497 (1974).

    Google Scholar 

  21. Yoshiki, T., Mellors, R. C., Hardy, W. D., Fleissner, E.: Common cell surface antigen associated with mammalian C-type viruses: Membrane-boundgs antigen. J. exp. Med.139, 925–942 (1974).

    Google Scholar 

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Authors and Affiliations

  1. Department of Veterinary Science, University of Wisconsin, Madison, Wisconsin, USA

    M. Onuma, D. M. Driscoll & C. Olson

  2. Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan

    M. Onuma

Authors
  1. M. Onuma
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  2. D. M. Driscoll
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  3. C. Olson
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Onuma, M., Driscoll, D.M. & Olson, C. Location of antigens associated with bovine leukemia virus. Archives of Virology 55, 131–137 (1977). https://doi.org/10.1007/BF01314486

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  • DOI: https://doi.org/10.1007/BF01314486

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