Summary
Cocultivation of fetal lamb kidney cells infected with bovine leukemia virus (BLV) and the cat cells containing murine sarcoma virus genome resulted in the rapid production of syncytia. This syncytia formation was inhibited by serum containing antibodies to glycoprotein antigen of BLV. When rabbit complement was added to the antiserum for early syncytia inhibition (ESI) test, a significant enhancement of ESI activity of the antiserum was observed. This enhancement was associated with IgG fraction but not with IgM fraction of the antiserum. The results of the comparative serological tests showed that the ESI test with complement was much more sensitive than either immunodiffusion or complement fixation tests in the detection of BLV antibodies.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aslund, O., Osebold, J. W., Murphy, F. A.: Isolation and characterization of ovine gamma globulins. Archiv. Biochem. Biophys.109, 142–149 (1965).
Diglio, C. A., Ferrer, J. F.: Induction of syncytia by the bovine C-type leukemia virus. Cancer Res.34, 893–900 (1976).
Greig, A. S., Chander, S., Samagh, B., Bouillant, A. M. P.: A simple, rapid syncytia-inhibition test for antibodies to bovine leukemia virus. Canad. J. comp. Med.42, 446–451 (1978).
Guillemain, B., Mamoun, R., Levy, D., Astier, T., Irgens, K., Parodi, A. A.: Early polykaryocytosis inhibition: A simple quantitativein vitro assay for the detection of bovine leukemia virus infection in cattle. Europ. J. Cancer.14, 811–827 (1978).
Honma, T., Onuma, M., Suneya, M., Mikami, T., Izawa, H.: Cytotoxic antibody in cattle and sheep exposed to bovine leukemia virus. Arch. Virol.66, 293–299 (1980).
Lowry, O. H., Rosebrough, N. J., Farr, L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem.193, 265–275 (1951).
Miller, J. M., van der Maaten, M. J.: Use of glycoprotein antigen in the immunodiffusion test for bovine leukemia virus antibodies. Europ. J. Cancer13, 1369–1375 (1977).
Ogura, H., Paulsen, J., Bauer, H.: Cross neutralization of ovine and bovine C-type leukemia virus-induced syncytia formation. Cancer Res.37, 1486–1489 (1977).
Onuma, M., Olson, C., Baumgartener, L. E., Pearson, L. D.: An ether-sensitive antigen associated with bovine leukemia virus infection. J. Natl. Cancer Inst.55, 1155–1158 (1975).
Onuma, M., Olson, C., Driscoll, D. M.: Properties of two isolated antigens associated with bovine leukemia virus infection. J. Natl. Cancer Inst.57, 571–578 (1976).
Onuma, M., Watarai, S., Mikami, T., Izawa, H.: Cell fusion activity of bovine leukaemia virus. J. gen. Virol.48, 421–424 (1980).
Onuma, M., Watarai, S., Sonoda, M., Mikami, T., Izawa, H.: Detection of bovine leukemia virus by syncytium assay. Canad. J. comp. Med.44, 289–293 (1980).
Yoshino, K., Hashimoto, M., Shinkai, K.: Studies on the neutralization of herpes simplex virus VIII. Significance of viral sensitization for inactivation by complement. Microbiol. Immunol.21, 231–241 (1977).
Yoshino, K., Taniguchi, S.: The appearance of complement-requiring neutralizing antibodies by immunization and infection with herpes simplex virus. Virology22, 193–202 (1964).
Author information
Authors and Affiliations
Additional information
With 1 Figure
Rights and permissions
About this article
Cite this article
Watarai, S., Onuma, M., Mikami, T. et al. Enhancement of antibody activity to bovine leukemia virus by complement. Archives of Virology 68, 135–142 (1981). https://doi.org/10.1007/BF01314443
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01314443