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Expression of an early Epstein-Barr virus antigen (EA-D) in E. coli

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Summary

The 1.34 kb BcII-BgIII-fragment of the BamHI-M region of Epstein-Barr virus genome, comprising the complete BMRF1 open reading frame [1], was cloned into the tryptophan regulated E. coli expression vector pATH1. The resulting fusion protein, having a molecular weight of 80kd, is recognized not only by anti-early antigen (EA)-positive human sera but also by the monoclonal antibody R3 directed against the diffuse component of EA (EA-D) [29]. A possible use for this fusion protein as an indicator protein in diagnosis of IgA antibodies against EA-D is presented.

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Authors and Affiliations

  1. Abteilung Virologie, Institut für Medizinische Mikrobiologie und Hygiene, Universität Freiburg, Federal Republic of Germany

    Dagmar Roeckel, H. Boos & N. Mueller-Lantzsch

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  1. Dagmar Roeckel
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  2. H. Boos
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  3. N. Mueller-Lantzsch
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Roeckel, D., Boos, H. & Mueller-Lantzsch, N. Expression of an early Epstein-Barr virus antigen (EA-D) in E. coli. Archives of Virology 97, 365–372 (1987). https://doi.org/10.1007/BF01314434

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  • DOI: https://doi.org/10.1007/BF01314434

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