Summary
The effect of β-decay of radionuclides incorporated into influenza virus on the properties of the two closely adjacent structures — RNA and nucleoprotein (NP) — was studied. The long-term storage of3H-uridine labelled influenza virus was shown to lead to the loss of infectivity. This effect may be explained by lethal intra-molecular modifications of viral RNA, caused by β-decay of3H incorporated into the molecule. There was an accompanying decrease of monoclonal antibody (MAB) binding activity, this also being a plausible result of β-decay. The different rates of inactivation of MAB binding activity of different epitopes of NP of the3H-labelled virus shown in our studies suggest that there are different types of structural organization or different location of these epitopes in the NP.
The effect of3H-decay on the intracellular RNA of reproducing virus lead to a decrease in virus yield; this may be due to radiation- and transmutation-induced damage of messenger and progeny RNA populations synthesized during the infection.
The storage of influenza virus labelled with14C-aminoacids lead to a decrease in MAB binding activity of the NP that was unaccompanied by a decrease in infectivity. Furthermore,14C-decay in proteins of reproducing virus had no adverse effect.
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Prokudina, E.N., Semyonova, N.P., Yamnikova, S.S. et al. Effect of β-decay of radionuclides incorporated into influenza virus RNA and proteins on the infectivity of the virus and antigenicity of its nucleoprotein. Archives of Virology 97, 325–332 (1987). https://doi.org/10.1007/BF01314430
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DOI: https://doi.org/10.1007/BF01314430