Archives of Virology

, Volume 97, Issue 3–4, pp 309–323 | Cite as

Characteristics of Australian human enteric coronavirus-like particles: comparison with human respiratory coronavirus 229E and duodenal brush border vesicles

  • R. D. Schnagl
  • Sharon Brookes
  • Sue Medvedec
  • Fran Morey
Original Papers

Summary

The polypeptide profiles of highly purified coronavirus-like particles (CVLPs) proved to be very different from that of human respiratory coronavirus 229E and showed the particles not to be coronaviruses. Differences in polypeptide profiles and morphology between the CVLPs and duodenal brush border vesicles suggested that the CVLPs were also not such vesicles.

Although they shared some basic overall similarity, the polypeptide profiles of three different but possibly antigenically identical CVLP preparations from Central Australian Aborigines were very dissimilar in detail. At least 38, 39 and 48 bands respectively were observed on the three profiles. At least 46 bands were visible on the polypeptide profile of CVLPs from a Vietnamese immigrant to Australia, and it also differed in detail from those of the Central Australian CVLPs.

Indications of antigenic difference were obtained between Central Australian CVLPs and CVLPs from India, Kiribati, South Africa and Vietnamese immigrants to Australia. Antigenic difference was also suggested between the Central Australian CVLPs and those from one distant location within Australia, but antigenic similarity with those from another was indicated.

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References

  1. 1.
    Anderson H, Christiansen G, Christiansen C (1984) Electrophoretic analysis of proteins fromMycoplasma capricolum and related serotypes using extracts from intact cells and from minicells containing cloned mycoplasma DNA. J Gen Microbiol 130: 1409–1418Google Scholar
  2. 2.
    Arita M, Tagaya I (1980) Virion polypeptides of poxviruses. Arch Virol 62: 209–225Google Scholar
  3. 3.
    Beards GM, Brown DWG, Green J, Flewett TH (1986) Preliminary characterisation of torovirus-like particles of humans: comparison with Berne virus of horses and Breda virus of calves. J Med Virol 20: 67–78Google Scholar
  4. 4.
    Caul EO, Ashley CR, Egglestone SI (1977) Recognition of human enteric coronaviruses by electron microscopy. Med Lab Sci 34: 259–263Google Scholar
  5. 5.
    Caul EO, Egglestone SI (1977) Further studies on human enteric coronaviruses. Arch Virol 54: 107–117Google Scholar
  6. 6.
    Caul EO, Paver WK, Clarke SKR (1975) Coronavirus particles in faeces in patients with gastroenteritis. Lancet i: 1192Google Scholar
  7. 7.
    Dourmashkin RR, Davies HA, Smith HA, Bird RG (1980) Are coronavirus-like particles seen in diarrhoeic stools really viruses? Lancet ii: 971–972Google Scholar
  8. 8.
    Feldner J, Bredt W (1983) Analysis of polypeptides of mutants ofMycoplasma pneumoniae that lack the ability to haemadsorb. J Gen Microbiol 129: 841–848Google Scholar
  9. 9.
    Gerna G, Passarani N, Battaglia M, Rondanelli EG (1985) Human enteric coronaviruses: antigenic relatedness to human coronavirus OC43 and possible etiologic role in viral gastroenteritis. J Infect Dis 151: 796–803Google Scholar
  10. 10.
    Herring AJ, Inglis NF, Ojeh CK, Snodgrass DR, Menzies JD (1982) Rapid diagnosis of rotavirus infection by direct detection of viral nucleic acid in silver-stained polyacrylamide gels. J Clin Microbiol 16: 473–477Google Scholar
  11. 11.
    Kapikian AZ, Gerlin JF, Wyatt RG, Thornhill TS, Chanock RM (1973) Density in caesium chloride of the 27 nm “8Flla” particle associated with acute non-bacterial gastroenteritis: determination by ultacentrifugation and immune electron microscopy. Proc Soc Exp Biol Med 142: 874–877Google Scholar
  12. 12.
    Kessler M, Acuto O, Storelli C, Murer H, Muller M, Semenza G (1978) A modified procedure for the rapid preparation of efficiently transporting vesicles from small intestinal brush border membranes. Their use in investigating some properties of D-glucose and chloline transport systems. Biochim Biophys Acta 506: 136–154Google Scholar
  13. 13.
    Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685Google Scholar
  14. 14.
    MacNaughton MR, Davies HA (1981) Human enteric coronaviruses. Brief review. Arch Virol 70: 301–313Google Scholar
  15. 15.
    Mathan M, Mathan VI, Swaminathan SP, Yesudoss S, Baker SJ (1975) Pleomorphic virus-like particles in human faeces. Lancet i: 1068–1069Google Scholar
  16. 16.
    Resta S, Luby JP, Rosenfeld CR, Siegel JD (1985) Isolation and propagation of a human enteric coronavirus. Science 229: 978–981Google Scholar
  17. 17.
    Schnagl RD, Greco T, Morey F (1986) Antibody prevalence to human enteric coronavirus-like particles and indications of antigenic differences between particles from different areas. Arch Virol 87: 331–337Google Scholar
  18. 18.
    Schnagl RD, Holmes IH, MacKay-Scollay EM (1978) Coronavirus-like particles in Aboriginals and non-Aboriginals in Western Australia. Med J Aust 1: 307–309Google Scholar
  19. 19.
    Schnagl RD, Morey F, Holmes IH (1979) Rotavirus, coronavirus-like particles, bacteria and parasites in Central Australia. Med J Aust 2: 115–118Google Scholar
  20. 20.
    Siddell SG, Wege H, Ter Meulen V (1982) The structure and replication of coronaviruses. In: Cooper M et al (eds) Current topics in microbiology and immunology, vol 99. Springer, Berlin Heidelberg New York, vol 99, pp 131–163Google Scholar
  21. 21.
    Sitbon M (1985) Human-enteric-coronavirus-like particles (CVLP) with different epidemiological characteristics. J Med Virol 16: 67–76Google Scholar
  22. 22.
    Takayama H, Katsumoto T, Ohno K, Nakaso A, Tagaki A, Kimura G (1983) In situ electron microscopic observation of negatively stained tissue culture cells contaminated with mycoplasmas. J Gen Microbiol 129: 3379–3384Google Scholar
  23. 23.
    Weiss M, Horzinek MC (1987) The proposed family Toroviridae: agents of enteric infections. Brief review. Arch Virol 92: 1–15Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • R. D. Schnagl
    • 1
  • Sharon Brookes
    • 1
  • Sue Medvedec
    • 1
  • Fran Morey
    • 2
  1. 1.Department of MicrobiologyLa Trobe UniversityBundoora
  2. 2.Pathology LaboratoryAlice Springs HospitalAlice SpringsAustralia

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