Summary
A mumps virus strain, which replicated in mouse lung after aerosol inhalation, was obtained by selective replication of a wild strain in L929 cells and by further passaging in mice by intraperitoneal inoculation. All of infected mice survived and rechallenge of the survived mice with the same virus resulted in no virus growth in the lung. Treatment of infected mice with antiserum against interferon (IFN) or asialo GM1 delayed virus clearance from lung. Mice at 5 weeks of age were also sensitive to the virus as well as those at 1 week. When injected intravenously, the virus could grow not only in lung but also in salivary glands, heart and spleen. Furthermore, the virus replicated in liver, spleen, pancreas and testis after intraperitoneal inoculation. Antibody response of mice infected by aerosol inhalation was slower than that of intraperitoneally infected ones in either IgG or IgM production. These results indicated that the adapted virus replicated in mouse lung by a natural route of infection and had a potential to cause systemic infection in mouse.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
De Maeyer E, de Maeyer-Guignard J (1979) Considerations on mouse genes influencing interferon production and action. In: Gresser I (ed) Interferon 1. Academic Press, London, pp 75–100
Feldman HA (1982) Mumps. In: Evans AS (ed) Viral infections of humans: epidemiology and control. Plenum Press, New York, pp 419–440
Fujita T, Saito S, Kohno S (1979) Priming increase the amount of interferon mRNA in Poly(rI)-Poly(rC)-treated L cells. J Gen Virol 45: 301–308
Habu S, Fukui H, Shimamura K, Kasai M, Nagai Y, Okumura K, Tamaoki N (1981) In vivo effects of anti-asialo GM1. I. Reduction of NK activity and enhancement of transplanted tumor growth in nude mice. J Immunol 127: 34–38
Hayashi K, Ross ME, Notkins AL (1976) Persistence of mumps viral antigens in mouse brain. Jpn J Exp Med 46: 197–200
Kasai M, Yoneda T, Habu S, Maruyama Y, Okumura K, Tokunaga T (1981) In vivo effect of anti-asialo GM1 antibody on natural killer activity. Nature 291: 334–335
Kilham L, Murphy HW (1952) Propagation of mumps virus in suckling mice and in mouse embryo tissue cultures. Proc Soc Exp Biol Med 80: 495–498
Kilham L, Overman JR (1953) Natural pathogenicity of mumps virus for suckling hamsters on intracerebral inoculation. J Immunol 70: 147–151
Kristensson K, Örvell C, Malm G, Norrby E (1984) Mumps virus infection of the developing mouse brain — appearance of structural virus proteins demonstrated with monoclonal antibodies. J Neuropathol Exp Neurol 43: 131–140
Natuk RJ, Welsh RM (1987) Accumulation and chemotaxis of natural killer/large granular lymphocytes at sites of virus replication. J Immunol 138: 877–883
Sakata H, Hishiyama M, Sugiura A (1984) Enzyme-linked immunosorbent assay compared with neutralization tests for evaluation of live mumps vaccines. J Clin Microbiol 19: 21–25
Shimokata K (1978) Studies on the pathogenicity of human-origin parainfluenza virus in the brain of mice. Microbiol Immunol 22: 535–543
Tsurudome M, Yamada A, Hishiyama M, Ito Y (1984) Replication of mumps virus in murine cells. Arch Virol 81: 13–24
Tsurudome M, Yamada A, Hishiyama M, Ito Y (1986) Monoclonal antibodies against the glycoproteins of mumps virus: fusion inhibition by anti-HN monoclonal antibody. J Gen Virol 67: 2259–2265
Wolinsky JS, Klassen T, Baringer JR (1976) Persistence of neuroadapted mumps virus in brains of newborn hamsters after intraperitoneal inoculation. J Infect Dis 133: 260–267
Wolinsky JS, Server AC (1985) Mumps virus. In: Fields BN, Knipe DM, Chanock RM, Melnick JL, Roizman B, Shope RE (eds) Virology. Raven Press, New York, pp 1255–1284
Wolinsky JS, Stroop WG (1978) Virulence and persistence of three prototype strains of mumps virus in newborn hamsters. Arch Virol 57: 355–359
Wolinsky JS, Waxham MN, Server AC (1985) Protective effects of glycoprotein-specific monoclonal antibodies on the course of experimental mumps virus meningoencephalitis. J Virol 53; 727–734
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tsurudome, M., Yamada, A., Hishiyama, M. et al. Replication of mumps virus in mouse: transient replication in lung and potential of systemic infection. Archives of Virology 97, 167–179 (1987). https://doi.org/10.1007/BF01314419
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01314419