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Unusual migration profiles of Japanese encephalitis virus structural polypeptides in SDS-polyacrylamide gel electrophoresis

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Summary

The migration profiles of JEV polypeptides in SDS-PAGE are influenced by electrophoretic conditions. The estimated molecular weights of N and M became smaller when lower gel concentration and longer electrophoretic run were adopted.

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References

  1. Shapiro, D., Brandt, W. E., Cardiff, R. D., Russell, P. K.: The proteins of Japanese encephalitis virus. Virology44, 379–388 (1971).

    Google Scholar 

  2. Kitano, T., Suzuki, K., Yamaguchi, T.: Morphological, chemical, and biological characterization of Japanese encephalitis virus virion and its hemagglutinin. J. Virol.14, 631–639 (1974).

    Google Scholar 

  3. Strauss, J. H., Jr., Burge, B. W., Darnell, J. E.: Sindbis virus infection of chick and hamster cells: Synthesis of virus-specific proteins. Virology37, 367–376 (1969).

    Google Scholar 

  4. Pedersen, C. E., Jr., Marker, S. C., Eddy, G. A.: Comparative studies on the structural proteins of selected group A arboviruses. Virology60, 312–314 (1974).

    Google Scholar 

  5. Weber, K., Osborn, M.: The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J. biol. Chem.244, 4406–4412 (1969).

    Google Scholar 

  6. McCombs, R. M.: Antigens specified by herpesviruses III. Viral-induced nuclear polypeptides. Virology57, 448–458 (1974).

    Google Scholar 

  7. Hedrick, J. L., Smith, A. J.: Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresis. Arch. Biochem. Biophys.126, 155–164 (1968).

    Google Scholar 

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Kitano, T. Unusual migration profiles of Japanese encephalitis virus structural polypeptides in SDS-polyacrylamide gel electrophoresis. Archives of Virology 54, 159–163 (1977). https://doi.org/10.1007/BF01314389

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  • DOI: https://doi.org/10.1007/BF01314389

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