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Type specificity and use of human sera for rapid typing of herpes simplex virus isolates by an indirect peroxidase-labeled antibody technique: a comparison with three other methods

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Summary

Human sera from patients showing seroconversion following a primary herpes simplex virus type 1 (HSV-1) or HSV type 2 (HSV-2) infection, as well as 19S and 7S fractions obtained from the same sera, have been used for typing 191 HSV isolates by an indirect peroxidase-labeled antibody (PLA) method. Typing has also been performed on all of the isolates using a microneutralization (MN) and the indirect hemagglutination (IHA) inhibition test. Furthermore, 30 isolates have been typed by a plaque method. Results obtained with the PLA method was in complete agreement with those of the other two procedures. The PLA method is rapid and simple, offers easy interpretation and a permanent record of results.

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Authors and Affiliations

  1. Viral Diagnostic Service, Department of Pathology, Georgetown University Medical Center, Washington, D.C., USA

    G. Gerna, Janet Ditmore & R. W. Chambers

  2. Istituto Malattie Infettive, Università di Pavia, 27100, Pavia, Italy

    G. Gerna

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  1. G. Gerna
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  2. Janet Ditmore
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  3. R. W. Chambers
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Gerna, G., Ditmore, J. & Chambers, R.W. Type specificity and use of human sera for rapid typing of herpes simplex virus isolates by an indirect peroxidase-labeled antibody technique: a comparison with three other methods. Archives of Virology 54, 119–130 (1977). https://doi.org/10.1007/BF01314384

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  • DOI: https://doi.org/10.1007/BF01314384

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