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Inability of various immune mechanisms to establishin vitro latent infection in sensory neurons infected by HSV-1

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Summary

Various immune mechanisms were tested for their ability to alter a normally productive infection of herpes simplex virus in cultures of dissociated rat sensory neurons. These mechanisms included anti-HSV antibody with and without the aid of complement, HSV-sensitized “T” cells, natural killer cells, and “K” cells plus anti-HSV antibody (ADCC reactions). Although both anti-HSV antibody plus complement and the ADCC mechanism significantly limited infectious HSV production, no mechanism was capable of preventing the eventual infection of the majority of neurons. It appears, therefore, that none of these mechanisms by themselves can convert a normally productive infection of sensory neurons in culture to a non-productive or latent infection.

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References

  1. Baringer, J. R., Swoveland, P.: Recovery of herpes-simplex virus from human trigeminal ganglions. N. Engl. J. Med.288, 648–650 (1973).

    Google Scholar 

  2. Cabrera, C. V., Wohlenberg, C., Openshaw, H., Rey-Mendez, M., Puga, A., Notkins, A. L.: Herpes simplex virus DNA sequences in the CNS of latently infect mice. Nature228, 288–290 (1979).

    Google Scholar 

  3. Cook, M. L., Stevens, J. G.: Pathogenesis of herpetic neuritis and ganglionitis in mice: evidence for intraaxonal transport of infection. Infect. Immun.7, 272–288 (1973).

    Google Scholar 

  4. Fraser, N. W., Lawrence, W. C., Wroblewska, Z., Gilden, D. H., Koprowski, H.: Herpes simplex type 1 DNA in human brain tissue. Proc. Natl. Acad. Sci. U.S.A.78, 6461–6465 (1981).

    Google Scholar 

  5. Galloway, D. A., Fenoglio, C., Shevchuk, M., McDougall, J. K.: Detection of herpes simplex RNA in human sensory ganglia. Virology95, 265–268 (1979).

    Google Scholar 

  6. Green, M. T., Courtney, R. J., Dunkel, E. C.: Detection of an immediate early herpes simplex virus type 1 polypeptide in trigeminal ganglia from latently infected animals. Infect. Immun.34, 987–992 (1981).

    Google Scholar 

  7. Handwerger, B. S., Schwartz, R. H.: Separation of murine lymphoid cells using nylon wool columns — recovery of the B-cell enriched population. Transplantation18, 544–548 (1974).

    Google Scholar 

  8. Hill, T. J., Field, H. J., Blyth, W. A.: Acute and recurrent infection with herpes simplex virus in the mouse: a model for studying latency and recurrent disease. J. gen. Virol.28, 341–353 (1975).

    Google Scholar 

  9. Julius, M. H., Simpson, E., Herzenberg, L. A.: A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur. J. Immunol.3, 645–649 (1973).

    Google Scholar 

  10. Kristensson, K., Svennerholm, B., Persson, L., Vahlne, A., Lycke, E.: Latent herpes simplex virus trigeminal ganglionic infection in mice and demyelination in the central nervous system. J. Neurol. Sci.43, 253–264 (1979).

    Google Scholar 

  11. McKendall, R. R., Vogelzang, N., Jackson, G. G.: Herpes virus latency in spinal ganglia of mice without illness. Proc. Soc. Exp. Biol. Med.146, 1093–1096 (1974).

    Google Scholar 

  12. Nesburn, A. B., Green, M. T., Radioti, M., Walker B.: Reliablein vivo model for latent herpes simplex virus reactivation with peripheral virus shedding. Infect. Immun.15, 722–775 (1977).

    Google Scholar 

  13. Openshaw, H., Asher, L. U. S., Wohlenberg, C., Sekizama, T., Notkins, A. L.: Acute and latent infection of sensory ganglia with herpes simplex virus: immune control and virus reactivation. J. gen. Virol.44, 205–215 (1979).

    Google Scholar 

  14. Openshaw, H., Puga, A., Notkins, A. L.: Herpes simplex virus infection in sensory ganglia: immune control, latency, and reactivation. Fed. Proc.38, 2660–2664 (1979).

    Google Scholar 

  15. Openshaw, H., Sekizawa, T., Wohlenberg, C., Notkins, A. L.: The role of immunity in latency and reactivation of herpes simplex virus. In:Nahmias, A. J., Dowdle, W. R., Schinazi, R. E. (eds.), The human herpes viruses: an interdisciplinary approach, 287–296. Elsevier North-Holland 1981.

  16. Stevens, J. G., Cook, M. L.: Latent herpes simplex virus in ganglia of mice. Science173, 843–845 (1971).

    Google Scholar 

  17. Tenser, R. B., Hsiung, G. D.: Pathogenesis of latent herpes simplex virus infection of the trigeminal ganglion in guinea pigs: effects of age, passive immunization, and hydrocortisone. Infect. Immun.16, 69–74 (1977).

    Google Scholar 

  18. Walz, M. A., Price, R. W., Notkins, A. L.: Latent ganglionic infection with herpes simplex virus types 1 and 2: viral reactivationin vivo after neurectomy. Science184, 1185–1187 (1974).

    Google Scholar 

  19. Warren, K. G., Brown, S. M., Wroblewski, Z., Gilden, D., Koprowski, H., Subak-Sharpe, J.: Isolation of latent herpes simplex virus from the superior cervival and vagus ganglions of human beings. N. Engl. J. Med.298, 1068–1069 (1978).

    Google Scholar 

  20. Ziegler, R. J., Herman, R. E.: Peripheral infection in culture of rat sensory neurons by herpes simplex virus. Infect. Immun.28, 620–623 (1980).

    Google Scholar 

  21. Ziegler, R. J., Pozos, R. S.: Effects of lectins on peripheral infection by herpes simplex virus of rat sensory neurons in culture. Infect. Immun.34, 588–595 (1981).

    Google Scholar 

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Authors and Affiliations

  1. Department of Medical Microbiology and Immunology, University of Minnesota, School of Medicine, Duluth, Minnesota, USA

    R. J. Ziegler

  2. Department of Physiology, University of Minnesota, School of Medicine, Duluth, Minnesota, USA

    R. S. Pozos

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  1. R. J. Ziegler
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  2. R. S. Pozos
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Ziegler, R.J., Pozos, R.S. Inability of various immune mechanisms to establishin vitro latent infection in sensory neurons infected by HSV-1. Archives of Virology 79, 123–130 (1984). https://doi.org/10.1007/BF01314310

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  • DOI: https://doi.org/10.1007/BF01314310

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