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Equine herpesvirus genomes: Heterogeneity of naturally occurring type 4 isolates and of a type 1 isolate after heterologous cell passage

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Summary

The restriction endonuclease DNA fingerprints of 20 low passage, epidemiologically unrelated isolates of equine herpesvirus 4 (equine rhinopneumonitis virus) showed considerable heterogeneity in certain fragments, the positions of which were assigned to quite restricted positions on the 141 kilobase (kb) genome. We note that the heterogeneity observed in the restriction endonuclease DNA fingerprints of EHV1 (equine abortion virus) and of pseudorabies virus also tend to map to these same restricted regions.

The restriction endonuclease DNA fingerprints of an EHV1 strain was invariant using low (<1) multiplicity of infection during 20 passages in equine cells but when adapted to hamster cells developed an approximately 0.8 kb deletion in the unique short region of the genome between 8 and 11 passages.

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Author notes

  1. A. A. Cullinane

    Present address: Department of Clinical Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, England

Authors and Affiliations

  1. School of Veterinary Science, University of Melbourne, Parkville, Victoria, Australia

    M. J. Studdert, D. R. Fitzpatrick & G. F. Browning

  2. MRC Virology Unit, Institute of Virology, University of Glasgow, Scotland

    A. A. Cullinane

  3. School of Biological Sciences, Macquarie University, Sydney, Australia

    J. M. Whalley

Authors
  1. M. J. Studdert
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  2. D. R. Fitzpatrick
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  3. G. F. Browning
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  4. A. A. Cullinane
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  5. J. M. Whalley
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Studdert, M.J., Fitzpatrick, D.R., Browning, G.F. et al. Equine herpesvirus genomes: Heterogeneity of naturally occurring type 4 isolates and of a type 1 isolate after heterologous cell passage. Archives of Virology 91, 375–381 (1986). https://doi.org/10.1007/BF01314296

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  • DOI: https://doi.org/10.1007/BF01314296

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