Summary
The growth, stability and seroprevalence in laboratory rodents of the two known strains of mouse adenovirus were compared. The FL strain of mouse adenovirus grew in both L 929 murine fibroblasts and in CMT-93 murine rectal carcinoma cells, whereas the K 87 strain grew only in CMT-93 cells. The bulk of the FL progeny virus was released from the host cells. K 87 virus was largely cell-associated. Both virus strains were stable at 37° C in liquid medium. The K 87 strain was completely inactivated after 5–15 minutes at 56° C, whereas FL infectivity was still detected after two hours at this temperature. Both virus strains were stable in the dessicated state for 14 days, although FL viability was more dependent on the presence of protein in the virus diluent. Seroepidemiologic data suggest that viruses antigenically related to mouse adenovirus are more prevalent among laboratory rats than among laboratory mice and that the virus(es) infecting rats differ from those infecting mice. Results of retrospective serologic testing suggest an association between mouse adenovirus and an outbreak of disease in a mouse breeding colony.
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Blailock ZR, Rabin ER, Melnick JL (1967) Adenovirus endocarditis in mice. Science 157: 69–70
Blailock ZR, Rabin ER, Melnick JL (1968) Adenovirus myocarditis in mice. An electron microscopic study. Exp Mol Pathol 9: 84–96
Burrage TG, Smith AL (1986) Demonstration of murine adenovirus fiber protein in embedded cells using tannic acid and protein A gold immunolabelling. J Virol Meth (in press)
Franks LM, Hemmings VJ (1978) A cell line from an induced carcinoma of mouse rectum. J Pathol 124: 35–38
Hartley JW, Rowe WP (1960) A new mouse virus apparently related to the adenovirus group. Virology 11: 645–647
Hashimoto K, Sugiyama T, Sasaki S (1966) An adenovirus from the feces of mice. I. Isolation and identification. Jpn J Microbiol 10: 115–125
Heck FC, Sheldon WG, Gleiser CA (1972) Pathogenesis of experimentally produced mouse adenovirus infection in mice. Am J Vet Res 33: 841–846
Hoenig EM, Margolis G, Kilham L (1974) Experimental adenovirus infection of the mouse adrenal gland. II. Electron microscopic observations. Am J Pathol 75: 375–386
Jacoby RO, Bhatt PN, Jonas AM (1979) Viral diseases. In:Baker HJ, Lindsey JR, Weisbroth SH (eds) The laboratory rat, vol I. Academic Press, New York, pp 307–328
Luethans TN, Wagner JE (1983) A naturally occurring intestinal mouse adenovirus infection associated with negative serologic findings. Lab Anim Sci 33: 270–272
Lussier G, Smith AL, Guenette D, Descoteaux J-P (1986) Serological relationship between mouse adenovirus strains FL and K 87. Lab Anim Sci (in press)
Margolis G, Kilham L, Hoenig EM (1974) Experimental adenovirus infection of the mouse adrenal gland. I. Light microscopic observations. Am J Pathol 75: 363–372
Otten JA, Tennant RW (1982) Mouse adenovirus. In:Foster HL, Small JD, Fox JG (eds) The mouse in biomedical research, vol II: diseases. Academic Press, New York, pp 335–340
Reed LJ, Muench H (1938) A simple method of estimating fifty percent endpoints. Am J Hyg 27: 493–497
Smith AL (1983) An immunofluorescence test for detection of serum antibody to rodent coronaviruses. Lab Anim Sci 33: 157–160
Takeuchi A, Hashimoto K (1976) Electron microscope study of experimental enteric adenovirus infection in mice. Infect Immun 13: 569–580
Van der Deen J, Mes A (1974) Serological classification of two mouse adenoviruses. Arch Ges Virusforsch 45: 386–387
Ward JM, Young DM (1976) Latent adenoviral infection of rats: Intranuclear inclusions induced by treatment with a cancer chemotherapeutic agent. J Am Vet Med Assoc 169: 952–953
Wigand R, Gelderblom H, Ozel M (1977) Biological and biophysical characteristics of mouse adenovirus, strain FL. Arch Virol 54: 131–142
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Smith, A.L., Winograd, D.F. & Burrage, T.G. Comparative biological characterization of mouse adenovirus strains FL and K 87 and seroprevalence in laboratory rodents. Archives of Virology 91, 233–246 (1986). https://doi.org/10.1007/BF01314283
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DOI: https://doi.org/10.1007/BF01314283