Summary
Virus released from cowpox infected cells was demonstrated by electronmicroscopy to be surrounded by an envelope not present on mature intracellular virus. Enveloped cowpox had an isopycnic density of 1.23 g/ml, was infectious and neutralized by antibodies specific for the envelope antigens of vaccinia virus but not neutralized by antibodies specific for intracellular naked vaccinia virus. Only 1 per cent of the total intracellular virus yield was released as enveloped virus. The major cowpox glycoprotein (76 K) and a 44 K glycoprotein did not comigrate with any vaccinia glycoproteins whereas cowpox glycoproteins at 42 K and 20–23 K did coelectrophores with vaccinia glycoproteins. All of the mentioned cowpox glycoproteins were precipitated by vaccinia antiserum.
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Payne, L.G. The existence of an envelope on extracellular cowpox virus and its antigenic relationship to the vaccinia envelope. Archives of Virology 90, 125–133 (1986). https://doi.org/10.1007/BF01314150
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DOI: https://doi.org/10.1007/BF01314150