Summary
The effect of lymphoblastoid interferon (IFN) on viral polypeptides synthesized in a Vero cell culture persistently infected with subacute sclerosing panencephalitis virus (SSPE-Vero) was compared to that of Vero cells infected with exogenous measles virus. After IFN treatment there was no significant decrease in the synthesis of SSPE viral proteins, but inhibition of synthesis of measles polypeptides was readily seen. In Vero and SSPE-Vero cells IFN was able to inhibit replication of Sindbis virus although the effect in Vero cells was significantly more sensitive. In both cell lines IFN was able to stimulate 2′–5′ oligoadenylate synthetase (E enzyme) but not the protein kinase system. The SSPE-Vero cells showed a lower basal level of E enzyme activity than the Vero cells.
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Bonner, W. M., Laskey, R. A.: A film detection method for tritium-labelled protein and nucleic acids in polyacrylamide gels. Eur. J. Biochem46, 83–88 (1974).
Carter, M. J., ter Meulen, V.: Subacute sclerosing panencephalitis: are antigenic changes involved in measles virus persistence? In:Behan, P.O., ter Meulen, V., Clifford Rose, F. (eds.): Immunology of nervous system infections (Progress in Brain Research, Vol. 59) 163–171 (1983).
Carter, M. J., Willcocks, M. M., ter Meulen, V.: Defective translation of measles virus matrix protein in a subacute sclerosing panencephalitis cell line. Nature305, 153–155 (1983).
Freeman, J. M., Magoffin, R. L., Lennette, E. H., Herndon, R. M.: Additional evidence of the relation between subacute inclusion-body encephalitis and measles virus. Lancetii, 129–131 (1967).
Giraudon, P., Gerald, Ch., Wild, T. F.: A study of measles virus antigens in acutely and persistently infected cells using monoclonal antibodies: differences in the accumulation of certain viral proteins. Intervirology21, 110–120 (1984).
Graves, M. C.: Measles virus polypeptides in infected cells studied by immunoprecipitation and one dimensional peptide mapping. J. Virol.38, 224–230 (1981).
Hall, W. W., Choppin, P. W.: Evidence for lack of synthesis of the M polypeptide of measles virus in brain cells in subacute sclerosing panencephalitis. Virology99, 443–447 (1979).
Hall, W. W., Choppin, P. W.: Measles-virus proteins in the brain tissue of patients with subacute sclerosing panencephalitis. N. E. J. M.304, 1152–1155 (1981).
Holmes, S. L., Gupta, S. L.: Interferon action in human fibroblasts: induction of 2′–5′ oligoadenylate synthetase in the absence of detectable protein kinase activity. Arch. Virol.72, 137–142 (1982).
Jacobson, S., McFarland, H. F.: Measles virus persistence in human lymphocytes: A role for virus-induced interferon. J. gen. Virol.63, 351–357 (1982).
Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature227, 680–685 (1970).
Lyons, S. F., Schoub, B. D., Chiu, M. N., Crespi, M.: An indirect radioimmunoassay for interferon. J. Virol. Methods5, 93–100 (1982).
Machamer, C. E., Hayes, E. C., Zweerink, H. J.: Cells infected with a cell-associated subacute sclerosing panencephalitis virus do not express M protein. Virology108, 515–520 (1981).
Martin, E. M., Reisinger, D. M., Hovanessian, A. G., Williams, B. R. G.: Procedures for the assay of (2′–5′) oligoadenylic acids and their derivatives in intact cells. Meth. Enzymol.79 B, 273–284 (1981).
Mountcastle, W. F., Choppin, P. W.: A comparison of the polypeptides of four measles virus strains. Virology78, 463–474 (1972).
Revel, M., Kimchi, A., Schulman, L., Fradin, A., Schuster, R., Yakobson, E., Chernajovsky, Y., Schmidt, A., Shure, A., Bendori, R.: Role of interferon-induced enzymes in the antiviral and antimitogenic effects of interferon. Ann. N. Y. Acad. Sci.350, 459–472 (1980).
Samuel, C. E.: Mechanism of interferon action: phosphorylation of protein synthesis initiation factor eIF-2 in interferon treated human cells by a ribosome associated kinase processing site specificity similar to hemin-regulated rabbit reticulocyte kinase. Proc. Natl. Acad. Sci. U.S.A.76, 600–604 (1979).
Samuel, C. E.: Procedures for measurement of phosphorylation of ribosome-associated proteins in interferon treated cells. Meth. Enzymol.79 B, 168–178 (1981).
Schattner, A., Merlin, G., Wallach, D., Rosenberg, H., Bino, T., Hahn, T., Levin, S., Revel, M.: Monitoring of interferon therapy by assay of 2′–5′ oligoadenylate synthetase in human peripheral white blood cells. J. Interferon Res.1, 587–593 (1981).
Stephenson, J. R., Siddell, S. G., ter Meulen, V.: Resistant and lytic infections with SSPE virus: a comparison of the synthesis of virus specific polypeptides. J. gen. Virol.57, 191–197 (1981).
Struthers, J. K., Swanepoel, R.: Identification of a major non-structural protein in the nuclei of Rift Valley Fever virus-infected cells. J. gen. Virol.60, 381–384 (1982).
Volckaert-Vervliet, G., Heremans, H., De Ley, M., Biliau, A.: Interferon induction and action in human lymphoblastoid cells infected with measles virus. J. gen. Virol.41, 459–466 (1978).
Wechsler, S. L., Fields, B. N.: Intracellular synthesis of measles virus-specified polypeptides. J. Virol.25, 285–297 (1978).
Wechsler, S. L., Meissner, H. C.: Measles and SSPE viruses: Similarities and differences. Prog. Med. Virol.28, 65–95 (1982).
Wild, T. F., Dugre, R.: Establishment and characterisation of a subacute sclerosing panencephalitis (measles) virus persistent infection in BGM cells. J. gen. Virol.39, 113–124 (1978).
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Crespi, M., Chiu, M.N., Schoub, B.D. et al. Effect of interferon on Vero cells persistently infected with SSPE virus and lytically infected with measles virus. Archives of Virology 90, 87–96 (1986). https://doi.org/10.1007/BF01314147
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DOI: https://doi.org/10.1007/BF01314147