Summary
Mill Door/79 virus, an orbivirus of the Kemerovo serogroup, Great Island Complex, was shown to induce in infected cells 10 segments of double-stranded RNA with a total molecular weight of 11.46×106 daltons. Using methyl mercuric hydroxide as a denaturing agent the double-stranded RNA was translated in a cell-free system producing 11 polypeptides, 10 of which co-migrated with those produced in Mill Door/79 virus infected Vero cells. The segments were separated and individually translated in a cell-free system allowing their coding assignments to be made. These assignments were confirmed by partial proteolysis.
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Spence, R.P., Pullin, J.S.K. & Moore, N.F. Proteins expressed by Mill Door/79 virus, a Kemerovo serogroup orbivirus transmitted by the ticksIxodes uriae . Archives of Virology 90, 53–62 (1986). https://doi.org/10.1007/BF01314144
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DOI: https://doi.org/10.1007/BF01314144