Summary
Two blocking tests were applied simultaneously to detect antibodies to cytomegalovirus (CMV) in human serum specimens. Two different peroxidase-labelled tracers were prepared from human sera taken either early during a primary CMV infection or late after recurrent infection. Sera of acute primary CMV cases effectively competed with the labelled antibodies of the “early” tracer but a rather weak inhibition was observed with the “late” tracer. Sera of cases with recurrent infection strongly inhibited both tracers. Immunoblot experiments were carried out to explain the mechanism of this differential inhibition.
Similar content being viewed by others
References
Bradford, M. M.: A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Analyt. Biochem.72, 248–254 (1976).
Griffiths, P. D., Stagno, S., Pass, R. F., Smith, R. J., Alford, C. A.: Infection with cytomegalovirus during pregnancy: specifi IgM antibodies as a marker of recent primary infection. J. Inf. Dis.145, 647–653 (1982).
Hamilton, J. D.: Cytomegalovirus and immunity. In:Melnik, L. J. (ed.), Monographs in Virology, 35–46. Basel: Karger 1982.
Nowak, B. C., Sullivan, P., Sarnow, R., Thomas, R., Bricourt, J. C., Fleckenstein, B., Levine, A. J.: Characterization of monoclonal antibodies and polyclonal immune sera directed against human cytomegalovirus virion proteins. Virology132, 325–338 (1984).
Pereira, L., Hoffman, M., Cremer, N.: Electrophoretic analysis of polypeptides immune precipitated from cytomegalovirus-infected cell extracts by human sera. Infect. Immun.36, 933–942 (1982).
Pereira, L., Stagno, S., Hoffman, M., Volanakis, J. E.: Cytomegalovirus-infected cell polypeptides immune precipitated by sera from children with congenital and perinatal infections. Infect. Immun.39, 100–108 (1983).
Schmitz, H., Haas, R.: Determination of different Cytomegalovirus Immunoglobulins (IgG, IgM, IgA) by Immunofluorescence. Arch. ges. Virusforsch.37, 131–140 (1972).
Schmitz, H., Doerr, H. W., Obrig, M.: Envelope and nucleocapid antigens of cytomegalovirus (CMV). Med. Microbiol. Immunol.161, 155–162 (1975).
Schmitz, H., Kampa, D., Doerr, H. W., Luthard, T., Hillemans, H. G.: IgM antibodies to cytomegalovirus during pregnancy. Arch. Virol.53, 177–184 (1977).
Schmitz, H., Mueller-Lantsch, N., Peteler, G.: Human antibody response to CMV nucleocapsid proteins. Intervirology13, 154–161 (1980).
Schmitz, H. U., von Deimling, U., Flemig, B.: Detection of IgM antibodies to cytomegalovirus (CMV) using an enzyme-labelled antigen (ELA). J. gen. Virol.50, 59–68 (1980).
Shope, R. E., Sather, G. E.: Arboviruses. In:Lennette, E. H., Schmidt, N. J. (eds.), Diagnostic Procedures for Viral, Rickettsial, and Chlamydial Infections, 767–814. Washington, D. C.: American Public Health Association, Inc. 1979.
Stagno, S., Pass, R. F., Dwordky, M. E., Henderson, R. E., Moore, E. G., Walton, P. D., Alford, C. A.: Congenital cytomegalovirus infection. The relative importance of primary and recurrent maternal infection. New Eng. J. Med.306, 945–949 (1982).
Stern, H., Tucker, S. M.: Prospective study of cytomegalovirus infection in pregnancy. Bri. Med. J.2, 268–270 (1973).
Stinsky, M. F.: Sequence of protein synthesis in cells infected by human cytomegalovirus. J. Virol.23, 751–767 (1977).
Towbin, H., Staehlelin, T., Gordon, J.: Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. U.S.A.76, 4350 (1979).
Wilchek, M., Bayer, E. A.: The avidin-biotin complex in immunology. Immunology Today5, 39–43 (1984).
Wilson, M. B., Nakane, P. K.: Recent developments in the peroxidase method of conjugating horseradish peroxidase (HRPO) to antibodies. In:Knapp, W. (ed.), Immunofluorescence and Related Techniques, 215–224. Amsterdam: Elsevier 1978.
Author information
Authors and Affiliations
Additional information
With 2 Figures
Rights and permissions
About this article
Cite this article
Schmitz, H., Mohr, H. & Majoubi, M. Differentiation between primary and recurrent cytomegalovirus infections. Archives of Virology 90, 41–51 (1986). https://doi.org/10.1007/BF01314143
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01314143