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Hepatitis B virus components produced by the human hepatoma cell line PLC/PRF/5: Do they indicate virus propagation?

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References

  1. Aden, D. P., Fogel, A., Plotkin, S., Damjanov, I., Knowles, B. B.: Controlled synthesis of HBsAg in a differentiated human liver carcinoma-derived cell line. Nature282, 615–616 (1979).

    Google Scholar 

  2. Albin, C., Robinson, W. S.: Protein kinase activity in hepatitis B virus. J. Virol.34, 297–302 (1980).

    Google Scholar 

  3. Alexander, J. J., Bey, E. M., Geddes, E. W., Lecatsas, G.: Establishment of a continuously growing cell line from primary carcinoma of the liver. S. Afr. Med. J.50, 2124–2128 (1976).

    Google Scholar 

  4. Beasley, R. P., Hwang, L. Y., Lin, C. C., Stevens, C. E., Wang, K. Y., Sun, T. S., Hsien, F. J., Szmuness, W.: Hepatitis B immune globulin (HBIG) efficacy in the interruption of perinatal transmission of hepatitis B virus carrier state. Lancetii, 388–393 (1981).

    Google Scholar 

  5. Beasley, R. P., Hwang, L. Y., Lin, C. C., Chien, C. S.: Hepatocellular carcinoma and HBV: a prospective study of 22,707 men in Taiwan. Lancetii, 1129–1133 (1981).

    Google Scholar 

  6. Blum, H. E., Haase, A. T., Harris, J. D., Walker, D., Vyas, G. N.: Asymmetric replication of hepatitis B virus DNA in human liver: Demonstration of cytoplasmic minus-strand DNA by blot analyses and in situ hybridization. Virology139, 87–96 (1984).

    Google Scholar 

  7. Cattaneo, R., Will, H., Hernandez, N., Schaller, H.: Signals regulating hepatitis B surface antigen transcription. Nature305, 336–338 (1983).

    Google Scholar 

  8. Cummings, I. W., Browne, J. K., Salser, W. A., Tyler, G. V., Snyder, R. L., Smolec, J. M., Summers, J.: Isolation, characterization and comparison of recombinant DNAs derived from genomes of human hepatitis B virus and woodchuck hepatitis virus. Proc. Natl. Acad. Sci. U.S.A.77, 1842–1846 (1980).

    Google Scholar 

  9. Dane, D. S., Cameron, C. H., Briggs, M.: Virus-like particles in the serum of patients with Australia antigen associated hepatitis. Lanceti, 695–698 (1970).

    Google Scholar 

  10. Dejean, A., Brechot, C., Tiollais, P., Wain-Hobson, S.: Characterization of integrated hepatitis B viral DNA cloned from a human hepatoma and the hepatoma-derived cell line PLC/PRF/5. Proc. Natl. Acad. Sci. U.S.A.80, 2505–2509 (1983).

    Google Scholar 

  11. Desmyter, J., Ray, M. B., Bradburne, A. F., Alexander, J. J.: Human HBsAg-positive hepatoma in nude athymic mice. In:Vyas, G. N., Cohen, S. N., Schmid, R. (eds.): Viral Hepatitis, 459–460. Philadelphia: Franklin Institute Press 1978.

    Google Scholar 

  12. Dubois, M. F., Pourcel, C., Rousset, S., Chany, C., Tiollais, P.: Excretion of hepatitis B surface antigen particles from mouse cells transformed with cloned viral DNA. Proc. Natl. Acad. Sci. U.S.A.77, 4549–4553 (1980).

    Google Scholar 

  13. Elfassi, E., Romet-Lemonne, J.-L., Essex, M., Frances-McLane, M., Haseltine, W. A.: Evidence of extrachromosomal forms of hepatitis B viral DNA in a bone marrow culture obtained from a patient recently infected with hepatitis B virus. Proc. Natl. Acad. Sci. U.S.A.81, 3526–3528 (1984).

    Google Scholar 

  14. Freytag von Loringhoven, A., Koch, S., Hofschneider, P. H., Koshy, R.: Cotranscribed 3′ host sequences augment expression of integrated hepatitis B virus DNA. EMBO J.4, 249–255 (1985).

    Google Scholar 

  15. Ganem, D.: Animal models in hepatitis research. In:Vyas, G. N., Dienstag, J. L., Hoofnagle, J. H. (eds.): Viral Hepatitis and Liver Disease, 439–441. Orlando: Grune and Stratton 1984.

    Google Scholar 

  16. Gerber, M. A., Garfinkel, E., Hirschman, S. Z., Thung, S. N., Panagiotatos, T.: Immune and enzyme histochemical studies of a human hepatocellular carcinoma cell line producing hepatitis B surface antigen. J. Immunol.126, 1085–1089 (1981).

    Google Scholar 

  17. Gerin, J. L., Shih, W.-K.: Structure of HBsAg and HBcAg. In:Vyas, G. N., Cohen, S. N., Schmid, R. (eds.): Viral Hepatitis, 147–158. Philadelphia: The Franklin Institute Press 1978.

    Google Scholar 

  18. Gerlich, W. H., Robinson, W. S.: Hepatitis B virus contains protein attached to the 5′ terminus of its complete DNA strand. Cell21, 801–809 (1980).

    Google Scholar 

  19. Gerlich, W. H., Goldmann, U., Müller, R., Stibbe, W., Wolff, W.: Specificity and localization of the hepatitis B virus-associated protein kinase. J. Virol.42, 761–766 (1982).

    Google Scholar 

  20. Gough, N. M., Murray, K.: Expression of the hepatitis B virus surface, core and e antigen by stable rat and mouse cell lines. J. Mol. Biol.162, 43–67 (1982).

    Google Scholar 

  21. Gough, N. M.: Core and e antigen synthesis in Rodent cells transformed with hepatitis B virus DNA is associated with greater than genome length viral messenger RNAs. J. Mol. Biol.165, 683–699 (1983).

    Google Scholar 

  22. Heermann, K. H., Goldmann, U., Schwartz, W., Seyffarth, T., Baumgarten, H., Gerlich, W. H.: Large surface proteins of hepatitis B virus containing the pre-s sequence. J. Virol.52, 396–402 (1984).

    Google Scholar 

  23. Hirschman, S. Z., Price, P., Garfinkel, E., Christman, J., Acs, G.: Expression of cloned hepatitis B virus DNA in human cell cultures. Proc. Natl. Acad. Sci. U.S.A.77, 5507–5511 (1980).

    Google Scholar 

  24. Hoefs, J. C., Renner, I. G., Ashcavai, M., Redecker, A. G.: Hepatitis surface antigen in pancreatic and bilary secretions. Gastroenterology79, 191–194 (1980).

    Google Scholar 

  25. Hofschneider, P. H., Marquardt, O., Wong, T.-K., Zaslavsky, V.: Structures specific for hepatitis B virus in cytoplasmic extracts of a hepatoma cell line producing hepatitis B surface antigen. Cold Spring Harbor Conferences on Cell Proliferation7, 435–446 (1980).

    Google Scholar 

  26. Hruska, J. F., Robinson, W. S.: The proteins of hepatitis B Dane particle cores. J. Med. Virol.1, 119–131 (1977).

    Google Scholar 

  27. Huh, N., Utakoji, T.: Production of HBs-antigen by two new human hepatoma cell lines and its enhancement by dexamethasone. Gann72, 178–179 (1981).

    Google Scholar 

  28. Jones, P. A., Taylor, A. M.: Cellular differentiation, cytidine analogs and DNA methylation. Cell20, 85–93 (1980).

    Google Scholar 

  29. Knowles, B. B., Howe, C. C., Aden, D. P.: Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science209, 497–499 (1980).

    Google Scholar 

  30. Koshy, R., Koch, S., Freytag v. Loringhoven, A., Kahmann, R., Murray, K., Hofschneider, P. H.: Integration of hepatitis B virus DNA: evidence for the integration in the single-stranded gap. Cell34, 215–223 (1983).

    Google Scholar 

  31. Machida, A., Kishimoto, S., Ohnuma, H., Miyamoto, H., Baba, K., Oda, K., Nakamura, T., Miyakawa, Y., Mayumi, M.: A hepatitis B surface antigen polypeptide (p31) with the receptor for polymerized human as well as chimpanzee albumins. Gastroenterol.85, 268–274 (1983).

    Google Scholar 

  32. Mackay, P., Lees, J., Murray, K.: The conversion of hepatitis B core antigen synthesized in E. coli into e antigen. J. Med. Virol.8, 237–243 (1981).

    Google Scholar 

  33. Macnab, G. M., Alexander, J. J., Lecatsas, G., Bey, E. M., Urbanowicz, J. M.: Hepatitis B surface antigen produced by a human hepatoma cell line. Br. J. Cancer34, 509–515 (1976).

    Google Scholar 

  34. Marion, P. L., Salazar, F. H., Alexander, J. J., Robinson, W. S.: Polypeptides of hepatitis B virus surface antigen produced by a hepatoma cell line. J. Virol.32, 796–802 (1979).

    Google Scholar 

  35. Marion, P. L., Oshiro, L. S., Regnery, D. C., Sculard, G. H., Robinson, W. S.: A virus in Beechey ground squirrels which is related to hepatitis B virus of man. Proc. Natl. Acad. Sci. U.S.A.77, 2941–2945 (1980).

    Google Scholar 

  36. Marquardt, O., Zaslavsky, V., Hofschneider, P. H.: Evidence for non-chromosomal hepatitis B virus surface (HBsAg)- and core antigen (HBcAg)-specific DNA sequences in a hepatoma cell line. J. gen. Virol.61, 105–109 (1982).

    Google Scholar 

  37. Marquardt, O., Freytag v. Loringhoven, A., Frösner, G. G.: Expression of hepatitis B virus core antigen gene is induced in human hepatoma cells by their growth in nude mice. J. gen. Virol.65, 1443–1448 (1984).

    Google Scholar 

  38. Marquardt, O., Freytag v. Loringhoven, A., Koshy, R., Hofschneider, P. H., Miller, K., Desmyter, J.: A virus antigen (HBsAg) indicates metastases of human carcinoma cells in the nude mouse. Med. Microbiol. Immunol.172, 233–241 (1984).

    Google Scholar 

  39. Marquardt, O., Freytag v. Loringhoven, A., Miller, K., Desmyter, J.: Two types of cells are present inin vitro andin vivo cultures of PLC/PRF/5 cells. In:Vyas, G. N., Dienstag, J. L., Hoofnagle, J. H. (eds.): Viral Hepatitis and Liver Disease, 669–669. Orlando: Grune and Stratton 1984.

    Google Scholar 

  40. Mason, W. S., Seal, G., Summers, J.: Virus of Pekin ducks with structural and biological relatedness to human hepatitis B virus. J. Virol.36, 829–836 (1980).

    Google Scholar 

  41. Miller, R. H., Robinson, W. S.: Integrated hepatitis B virus DNA sequences specifying the major viral core polypeptide are methylated in PLC/PRF/5 cells. Proc. Natl. Acad. Sci. U.S.A.80, 2534–2538 (1983).

    Google Scholar 

  42. Miller, R. H., Tran, C.-T., Robinson, W. S.: Hepatitis B virus particles of plasma and liver contain viral DNA-RNA hybrid molecules. Virology139, 53–63 (1984).

    Google Scholar 

  43. Miyakawa, Y., Mayumi, M.: Characterization and clinical significance of HBeAg. In:Vyas, G. N., Cohen, S. N., Schmid, R. (eds.): Virol Hepatitis, 193–201. Philadelphia: The Franklin Institute Press 1978.

    Google Scholar 

  44. Moriarty, A. M., Alexander, H., Lerner, R., Thornton, G. B.: Antibodies to peptides detect new hepatitis B antigen: Serological correlation with hepatocellular carcinoma. Science227, 429–433 (1985).

    Google Scholar 

  45. Ono, Y., Onda, H., Sasada, R., Igarashi, K., Sugino, Y., Nishioka, K.: The complete nucleotide sequence of the cloned hepatitis B virus DNA subtype adr and adw. Nucl. Acid Res.11, 1747–1757 (1983).

    Google Scholar 

  46. Peterson, D. L., Roberts, I. M., Vyas, G. N.: Partial amino acid sequence of two major component polypeptides of hepatitis B surface antigen. Proc. Natl. Acad. Sci. U.S.A.74, 1530–1534 (1977).

    Google Scholar 

  47. Razin, A., Riggs, A. D.: DNA methylation and gene function. Science210, 604–610 (1980).

    Google Scholar 

  48. Robinson, W. S.: Hepatitis B Dane particle DNA structure and the mechanism of the endogenous DNA polymerase reaction. In:Vyas, G. N., Cohen, S. N., Schmid, R. (eds.): Viral Hepatitis, 139–145. Philadelphia: The Franklin Institute Press 1978.

    Google Scholar 

  49. Romet-Lemonne, J.-L., McLane, M. F., Elfassi, E., Haseltine, W. A., Azocar, J., Essex, M.: Hepatitis B virus infection in cultured human lymphoblastoid cells. Science221, 667–669 (1983).

    Google Scholar 

  50. Rutter, W. J., Ziemer, M., Ou, J., Shaul, Y., Laub, O., Garcia, P., Standring, D. N.: Transcription units of hepatitis B virus genes and structure and expression of integrated viral sequences. In:Vyas, G. N., Dienstag, J. L., Hoofnagle, J. H. (eds.): Viral Hepatitis and Liver Disease, 67–86. Orlando: Grune and Stratton 1984.

    Google Scholar 

  51. Sattler, F. R., Paolucci, S., Kreider, J. W., Ladda, R. L.: A human hepatoma cell line (PLC/PRF/5) produces lung metastases and secretes HBsAg in nude mice. Eur. J. Cancer Clin. Oncol.18, 381–389 (1982).

    Google Scholar 

  52. Skelly, J., Copeland, J. A., Howard, C. R., Zuckerman, A. J.: Hepatitis B surface antigen produced by a human hepatoma cell line. Nature282, 617–618 (1979).

    Google Scholar 

  53. Southern, E. M.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol.98, 503–517 (1975).

    Google Scholar 

  54. Stibbe, W., Gerlich, W. H.: Structural relationships between minor and major proteins of hepatitis B surface antigen. J. Virol.46, 626–628 (1983).

    Google Scholar 

  55. Summers, J., Smolec, J., Snyder, R.: A virus similar to human hepatitis B virus associated with hepatitis and hepatoma in woodchucks. Proc. Natl. Acad. Sci. U.S.A.75, 4533–4537 (1978).

    Google Scholar 

  56. Szmuness, W., Harley, E. J., Ikram, H., Stevens, C. E.: Sociodemographic aspects of the epidemiology of hepatitis B. In:Vyas, G. N., Cohen, S. N., Schmid, R. (eds.): Viral Hepatitis, 297–320. Philadelphia: The Franklin Institute Press 1978.

    Google Scholar 

  57. Takahashi, K., Machida, A., Funatsu, G., Nomura, M., Usuda, S., Aoyagi, S., Tachibana, K., Miyamoto, H., Imai, M., Nakamura, T., Miyakawa, Y., Mayumi, M.: Immunochemical structure of hepatitis B e antigen in the serum. J. Immunol.130, 2903–2907 (1983).

    Google Scholar 

  58. Thomas, H. C., Pignatelli, M., Goodall, A., Waters, J., Karayannis, P., Brown, D.: Immunologic mechanisms of cell lysis in hepatitis B virus infection. In:Vyas, G. N., Dienstag, J. L., Hoofnagle, J. H. (eds.): Viral Hepatitis and Liver Disease, 167–177. Orlando: Grune and Stratton 1984.

    Google Scholar 

  59. Toh, H., Hyashida, H., Miyata, T.: Sequence homology between retroviral reverse transcriptase and putative polymerases of hepatitis B virus and cauliflower mosaic virus. Nature305, 827–829 (1983).

    Google Scholar 

  60. Valenzuela, P., Gray, P., Quiroga, M., Zaldivar, J., Goodman, H. M., Rutter, W. J.: Nucleotide sequence of the gene coding for the major protein of hepatitis B virus surface antigen. Nature280, 815–819 (1979).

    Google Scholar 

  61. Wain-Hobson, S., Tiollais, P.: Hepatitis B virus. In:O'Brien, S. J. (ed.): Genetic Maps3, 92–98. Cold Spring Harbor Laboratory 1984.

    Google Scholar 

  62. Wang, Y., Schäfer-Ridder, M., Stratowa, C., Wong, T.-K., Hofschneider, P. H.: Expression of hepatitis B surface antigen in unselected cell culture transfected with recircularized HBV DNA. EMBO J.1, 1213–1216 (1982).

    Google Scholar 

  63. Will, H., Cattaneo, R., Koch, H.-G., Darai, G., Schaller, H., Schellekens, H., van Eerd, P. M. C. A., Deinhardt, F.: Cloned HBV DNA causes hepatitis in chimpanzees. Nature299, 740–742 (1982).

    Google Scholar 

  64. Yoakum, G. H., Korba, B. E., Lechner, J. F., Tokiwa, T., Gazdar, A. F., Seeley, T., Siegel, M., Leeman, L., Autrup, H., Harris, C. C.: High-frequency transfection and cytopathology of the hepatitis B virus core antigen gene in human cells. Science222, 385–389 (1983).

    Google Scholar 

  65. Zaslavsky, V., Marquardt, O., Wong, T.-K., Hofschneider, P. H.: Hepatitis B virus (HBV)-specific structures found in cytoplasmic extracts of cells producing HBV surface antigen (HBsAg)in vitro. J. gen. Virol.51, 341–349 (1980).

    Google Scholar 

  66. Ziemer, M., Garcia, P., Shaul, Y., Rutter, W. J.: Sequence of hepatitis B virus DNA incorporated into the genome of a human hepatoma cell line. J. Virol.53, 885–892 (1985).

    Google Scholar 

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  1. Department of Virology, Max-Planck-Institut für Biochemie, Martinsried, Germany

    O. Marquardt

  2. Federal Research Centre for Virus Diseases of Animals, P.O.Box 1149, D-7400, Tübingen, Germany

    O. Marquardt

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Marquardt, O. Hepatitis B virus components produced by the human hepatoma cell line PLC/PRF/5: Do they indicate virus propagation?. Archives of Virology 90, 1–14 (1986). https://doi.org/10.1007/BF01314140

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