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Purification of rubella virus by isopycnic gradients: continuous Percoll versus discontinuous sucrose

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Summary

Concentrated viral suspensions were purified by isopycnic gradients: discontinuous sucrose, continuous Percoll. With Percoll, homogenous buoyant density virus were obtained; hemagglutinating titer, concentration factor, specific activity, and reproducible yield were higher than with sucrose.

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Authors and Affiliations

  1. Laboratorio de Virología, Edificio “A” Segundo Piso, Facultad de Medicina, Universidad Nacional Autónoma de México, México City, México

    J. Bustos, P. Zamora, E. Mejía, Y. Varela & B. Gómez

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  1. J. Bustos
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  2. P. Zamora
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  3. E. Mejía
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  4. Y. Varela
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  5. B. Gómez
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Bustos, J., Zamora, P., Mejía, E. et al. Purification of rubella virus by isopycnic gradients: continuous Percoll versus discontinuous sucrose. Archives of Virology 118, 285–288 (1991). https://doi.org/10.1007/BF01314039

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  • DOI: https://doi.org/10.1007/BF01314039

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