Summary
The genetic relationship between human and porcine Gp C rotaviruses and between Gp C and Gp A or B rotaviruses was examined by Northern blot hybridization. Cross-hybridization studies using radiolabeled ssRNA transcript probes demonstrated that the human and porcine Gp C rotaviruses shared a high degree of nucleotide sequence homology in most of the eleven gene segments; the greatest sequence divergence was observed in gene 7. Neither the human nor the porcine Gp C probe hybridized strongly with gene segments from Gp A reference strains or a Gp B bovine rotavirus. These data indicate that genetically, porcine and human Gp C rotaviruses are closely related, whereas they are quite distinct from Gp A or B suggesting that porcine and human Gp C rotaviruses may have evolved from a common ancestral source.
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References
Beards GM, Desselberger U, Flewett TH (1989) Temporal and geographical distributions of human rotavirus serotypes, 1983–1988. J Clin Microbiol 27: 2827–2833
Bohl EH, Saif LJ, Theil KW, Agnes AG, Cross RF (1982) Porcine pararotavirus: detection, differentiation from rotavirus, and pathogenesis in gnotobiotic pigs. J Clin Microbiol 15: 312–319
Bonsdorf CHV, Svensson L (1988) Human serogroup C rotavirus in Finland. Scand J Infect Dis 20: 475–478
Bremont M, Chabanne-Vautherot D, Vannier P, McCrae MA, Cohen J (1990) Sequence analysis of the gene (6) encoding the major capsid protein (VP 6) of group C rotavirus: higher than expected homology to the corresponding protein from group A virus. Virology 178: 579–583
Bridger JC (1987) Novel rotavirus in animals and man. In: Novel diarrhea viruses. Ciba Found Symp 128: 5–23
Bridger JC, Pedley S, McCrae MA (1986) Group C rotaviruses in humans. J Clin Microbiol 23: 760–763
Brown DWG, Mathan MM, Mathew M, Martin R, Beards GM, Mathan VI (1988) Rotavirus epidemiology in Vellore, South India: group, subgroup, serotype, and electropherotype. J Clin Microbiol 26: 2410–2414
Caul EO, Ashley CR, Darville JM, Bridger JC (1990) Group C rotavirus associated with fatal enteritis in a family outbreak. J Med Virol 30: 201–205
Chen G, Fan R, Guo X, Hung T (1988) Group C rotavirus found in sporadic diarrhea in China. J Exp Clin Virol 2: 1–3 (In Chinese with English abstract)
Eiden J, Vonderfecht S, Theil K, Torres-Medina A, Yolken RH (1986) Genetic and antigenic relatedness of human and animal strains of antigenically distinct rotaviruses. J Infect Dis 154: 972–982
Estes MK, Cohen J (1989) Rotavirus gene structure and function. Microbiol Rev 53: 410–499
Flores J, Myslinski J, Kalica AR, Greenberg HB, Wyatt RG, Kapikian AZ, Chanock RM (1982) In vitro transcription of two human rotaviruses. J Virol 43: 1032–1037
Hung T, Wang C, Fang Z, Chou Z, Chang X, Liong X, Chen G, Yao H, Chao T, Ye W, Den S, Chang W (1984) Waterborne outbreak of rotavirus diarrhea in adults in China caused by a novel rotavirus. Lancet i: 1139–1142
Ishimaru Y, Nakano S, Nakano H, Oseto M, Yamashita Y (1990) Group C rotavirusassociated gastroenteritis. Arch Pediatr Jap (in press)
Jashes M, Sandino AM, Faundez G, Avendano LF, Spencer E (1986) In vitro transcription of human pararotavirus. J Virol 57: 183–190
Jiang BM, Saif LJ, Kang SY, Kim JH (1990) Biochemical characterization of the structural and nonstructural polypeptides of a porcine group C rotavirus. J Virol 64: 3171–3178
Kapikian AZ, Chanock RM (1990) In: Fields BN et al (eds) Virology, 2nd edn. Raven Press, New York, pp 1353–1404
Kapikian AZ, Flores J, Midthun K, Hoshino Y, Green KY, Gorziglia M, Nishikawa K, Chanock RM, Potash LP, Perez-Schael L (1989) Strategies for the development of a rotavirus vaccine against infantile diarrhea with an update on clinical trials of rotavirus vaccines. Adv Exp Med Biol 257: 67–89
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685
Matsumoto K, Hatano M, Kobayashi K, Hasegawa A, Yamazaki S, Nakata S, Chiba S, Kimura Y (1989) An outbreak of gastroenteritis associated with acute rotaviral infection in schoolchildren. J Infect Dis 160: 611–615
Midthun K, Valdesuso J, Hoshino Y, Flores J, Kapikian AZ, Chanock RM (1987) Analysis by RNA-RNA hybridization assay of intertypic rotaviruses suggests that gene reassortment occurs in vivo. J Clin Microbiol 25: 295–300
Penaranda ME, Cubitt WD, Sinarachatanant P, Taylor DN, Likanonsakul S, Saif L, Glass RI (1989) Group C rotavirus infections in patients with diarrhea in Thailand, Nepal, and England. J Infect Dis 160: 392–397
Saif LJ, Theil KW (1985) Antigenically distinct rotaviruses of human and animal origin. In: Tzipori S (ed) Infectious diarrhoea in the young: strategies for control in humans and animals. Elsevier, Amsterdam, pp 208–214
Saif LJ, Terrett LA, Miller KL, Gross RF (1988) Serial propagation of porcine group C rotavirus (pararotavirus) in a continuous cell line and characterization of the passaged virus. J Clin Microbiol 26: 1277–1282
Saif LJ (1990) Nongroup A rotaviruses. In: Saif LJ, Theil KW (eds) Viral diarrheas of man and animals. CRC Press, Boca Raton, pp 73–95
Street JE, Croxson MC, Chadderton WF, Bellamy AR (1982) Sequence diversity of human rotavirus strains investigated by Northern blot hybridization analysis. J Virol 43: 369–378
Tanaka TN, Conner ME, Graham DY, Estes MK (1988) Molecular characterization of three rabbit rotavirus strains. Arch Virol 98: 253–265
Ushijima H, Honma H, Mukoyama A, Shinozaki T, Fujita Y, Kobayashi M, Ohseto M, Morikawa S, Kitamura T (1989) Detection of group C rotavirus in Tokyo. J Med Virol 27: 299–303
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Qian, Y., Saif, L.J., Kapikian, A.Z. et al. Comparison of human and porcine group C rotaviruses by Northern blot hybridization analysis. Archives of Virology 118, 269–277 (1991). https://doi.org/10.1007/BF01314037
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DOI: https://doi.org/10.1007/BF01314037