Summary
The presence of HIV-1 proviral DNA was determined in CD4 cells, CD8 cells and macrophages/monocytes obtained from peripheral blood of 8 HIV-1 infected persons. Using the polymerase chain reaction (PCR) we were able to detect proviral DNA in the extracts of only 102–103 CD4 (T4) cells. In contrast, 105 CD8 cells did not contain detectable amounts of proviral DNA. Surprisingly, in four of our eight patients studied no HIV-1 DNA was found in macrophages. In peripheral blood, every hundred T4 cell may be infected with HIV-1.
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References
Abbott MA, Poiesz BJ, Byrne BC, Kwok S, Sninsky JJ, Ehrlich GD (1988) Enzymatic gene amplification: qualitative and quantitative methods for detecting proviral DNA amplified in vitro. J Inf Dis 158: 1158–1169
Fleisher TA, Marti GE, Hagengruber C (1988) Two-color flow cytometric analysis of monocyte depleted human blood lymphocyte subsets. Cytometry 9: 303–315
Harper ME, Marselle LM, Gallo RC, Wong-Staal F (1986) Detection of lymphocytes expressing HTLV III in lymphnodes and in peripheral blood from infected individuals by in situ hybridization. Proc Natl Acad Sci USA 83: 772–776
Hart C, Spira T, Moore J, Sninsky J, Schochetman G, Lifson A, Galphin J, Ou CY (1988) Direct detection of HIV RNA expression in seropositive subjects. Lancet ii: 596–599
Jackson JB, Coombs RW, Sannerud K, Rhame FS, Balfour HH (1988) Rapid and sensitive viral cutlure method for human immunodeficiency virus type 1. J Clin Microbiol 26: 1416–1418
Klatzmann D, Barré-Sinoussi F, Nugeyere MT, Dauguet C, Jilmar E, Griscelli C, Brun-Vezinet F, Rouziaux C, Gluckman JC, Cherman JC, Montagnier L (1984) Selective tropism of lymphadenopathy associated virus (LAV) for helper-inducer T-lymphocytes. Science 225: 59–63
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Habour Laboratory, Cold Spring Habour, New York, p 191
Ou CY, Kwok S, Mitchell W, Mack DH, Sninsky JW, Krebs W, Feorino P, Warfield D, Schochetman G (1988) DNA amplification for the direct detection of HIV-1 in DNA of peripheral blood mononuclear cells. Science 239: 295–297
Popovic M, Gartner S (1987) Isolation of HIV-1 from monocytes but not from T lymphocytes. Lancet ii: 916
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Hufert, F.T., Laer, D.v., Schramm, C. et al. Detection of HIV-1 DNA in different subsets of human peripheral blood mononuclear cells using the polymerase chain reaction. Archives of Virology 106, 341–345 (1989). https://doi.org/10.1007/BF01313963
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DOI: https://doi.org/10.1007/BF01313963