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Reiterated sequences of herpes simplex virus type 1 (HSV-1) genome can serve as physical markers for the differentiation of HSV-1 strains

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Summary

The stability of regions containing tandemly reiterated sequences in the S component of the herpes simplex virus type 1 (HSV-1) genome was determined, by comparing restriction fragments of the regions among sets of HSV-1 isolates derived from a single source. The 6 reiterations examined were grouped into three. Reiteration VII (within protein coding regions of genes US10 and US11) and reiteration IV (within introns of genes US1 and US12) were stable between the isolates (group 1). Regions containing one of four other reiterations were detected as a set of ladder-like fragments. Reiteration II (between “a” sequence and IE175 gene) and reiteration VI (within an intergenic region on the 3′ side of the 3′ co-terminal family of genes US10, US11, and US12) (group 3) were more unstable than reiteration I (within “a” sequence) and reiteration III (between “a” sequence and IE175 gene) (group 2). The mode of fluctuation of the reiterations observed within a set of HSV-1 strains isolated from an individual was similar to that observed between HSV-1 single-plaque clones separated in cultured cells. These reiterations, except for group 3, can serve as sensitive and convenient markers for differentiating HSV-1 strains.

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References

  1. Buchman TG, Roizman B, Adams G, Stover BH (1978) Restriction endonuclease fingerprinting of herpes simplex virus DNA: a novel epidemiological tool applied to a nosocomial outbreak. J Infect Dis 138: 488–498

    Google Scholar 

  2. Buchman TG, Roizman B, Nahmias AJ (1979) Demonstration of exogenous genital reinfection with herpes simplex virus type 2 by restriction endonuclease fingerprinting of viral DNA. J Infect Dis 140: 295–304

    Google Scholar 

  3. Buchman TG, Simpson T, Nosal C, Roizman B (1980) The structure of herpes simplex virus DNA and its application to molecular epidemiology. Ann NY Acad Sci 354: 279–290

    Google Scholar 

  4. Chaney SMJ, Warren KG, Kettyls J, Zbitnue A, Subak-Sharpe JH (1983) A comparative analysis of restriction enzyme digests of the DNA of herpes simplex virus isolated from genital and facial lesions. J Gen Virol 64: 357–371

    Google Scholar 

  5. Chou J, Roizman B (1986) The terminala sequence of the herpes simplex virus genome contains the promoter of a gene located in the repeated sequences of the L component. J Virol 57: 629–637

    Google Scholar 

  6. Darville JM, Harbour DA, Hill TJ, Blyth WA (1987) Restriction endonuclease analysis of herpes simplex virus from recrudescent lesions, from latent infection and during passage in the skin and nervous system of mice. J Gen Virol 68: 907–911

    Google Scholar 

  7. Davison AJ, Wilkie NW (1981) Nucleotide sequences of the joint between the L and S segments of herpes simplex virus types 1 and 2. J Gen Virol 55: 315–331

    Google Scholar 

  8. Deiss LP, Chou J, Frenkel N (1986) Functional domains within thea sequence involved in the cleavage-packaging of herpes simplex virus DNA. J Virol 59: 605–618

    Google Scholar 

  9. Gerson M, Portnoy J, Hamelin C (1984) Consecutive infections with herpes simplex virus types 1 and 2 within a three-week period. J Infect Dis 149: 655

    Google Scholar 

  10. Halperin SA, Hendley JO, Nosal C, Roizman B (1980) DNA fingerprinting in investigation of apparent nosocomial acquisition of neonatal herpes simplex. J Pediatr 97: 91–93

    Google Scholar 

  11. Hayward GS, Frenkel N, Roizman B (1975) Anatomy of herpes simplex virus DNA: strain differences and heterogeneity in the locations of restriction endonuclease cleavage sites. Proc Natl Acad Sci USA 72: 1768–1772

    Google Scholar 

  12. Hubenthal-Voss J, Roizman B (1985) Herpes simplex virus 1 reiterated S component sequence (c1) situated between thea sequence anda4 gene are not essential for virus replication. J Virol 54: 509–514

    Google Scholar 

  13. Lonsdale DM, Brown SM, Lang J, Subak-Sharpe JH, Koprowski H, Warren KG (1980) Variations in herpes simplex virus isolated from human ganglia and a study of clonal variation in HSV-1. Ann NY Acad Sci 354: 291–308

    Google Scholar 

  14. Lonsdale DM, Brown SM, Subak-Sharpe JH (1979) The polypeptide and the DNA restriction enzyme profiles of spontaneous isolates of herpes simplex virus type 1 explants of human trigeminal, superior cervical and vagus ganglia. J Gen Virol 43: 151–171

    Google Scholar 

  15. Maitland NJ, Smith IW, Peutherer JH, Robertson DHH, Jones KW (1982) Restriction endonuclease analysis of DNA from genital isolates of herpes simplex virus type 2. Infect Immun 38: 834–842

    Google Scholar 

  16. McGeoch DJ, Dolan A, Donald S, Rixon FJ (1985) Sequence determination and genetic content of the short unique region in the genome of herpes simplex virus type 1. J Mol Biol 181: 1–13

    Google Scholar 

  17. McGeoch DJ, Dolan A, Donald S, Brauer DHK (1986) Complete DNA sequence of the short repeat region in the genome of herpes simplex virus type 1. Nucleic Acids Res 14: 1727–1745

    Google Scholar 

  18. Mocarski ES, Post LE, Roizman B (1980) Molecular engineering of the herpes simplex virus genome: insertion of a second L-S junction into the genome causes additional genome inversions. Cell 22: 243–255

    Google Scholar 

  19. Mocarski ES, Roizman B (1981) Site-specific inversion sequence of the herpes simplex virus genome: domain and structural features. Proc Natl Acad Sci USA 78: 7047–7051

    Google Scholar 

  20. Rixon FJ, McGeoch DJ (1984) A 3′ co-terminal family of mRNAs from the herpes simplex virus type 1 short region: two overlapping reading frames encode unrelated polypeptides one of which has a highly reiterated amino acid sequence. Nucleic Acids Res 12: 2473–2487

    Google Scholar 

  21. Rixon FJ, Campbell ME, Clements B (1984) A tandemly reiterated DNA sequence in the long repeated region of herpes simplex virus type 1 found in close proximity to immediate-early mRNA 1. J Virol 52: 715–718

    Google Scholar 

  22. Roizman B (1979) The structure and isomerization of herpes simplex virus genomes. Cell 16: 481–494

    Google Scholar 

  23. Sakaoka H, Aomori T, Ozaki I, Ishida S, Fujinaga K (1984) Restriction endonuclease cleavage analysis of herpes simplex virus isolates obtained from three pairs of siblings. Infect Immun 43: 771–774

    Google Scholar 

  24. Sakaoka H, Saheki Y, Uzuki K, Nakakita T, Saito H, Sekine K, Fujinaga K (1986) Two outbreaks of herpes simplex virus type 1 nosocomial infection among newborns. J Clin Microbiol 24: 36–40

    Google Scholar 

  25. Sakaoka H, Aomori T, Saito H, Sato S, Kawana R, Hazlett DT, Fujinaga K (1986) A comparative analysis by restriction endonucleases of herpes simplex virus type 1 isolated in Japan and Kenya. J Infect Dis 153: 612–616

    Google Scholar 

  26. Sakaoka H, Saito H, Sekine K, Aomori T, Grillner L, Wadell G, Fujinaga K (1987) Genomic comparison of herpes simplex virus type 1 isolates from Japan, Sweden, and Kenya. J Gen Virol 68: 749–764

    Google Scholar 

  27. Skare J, Summers WP, Summers WC (1975) Structure and function of herpesvirus genomes. I. Comparison of five HSV-1 and two HSV-2 strains by cleavage of their DNA withEcoRI restriction endonuclease. J Virol 15: 726–732

    Google Scholar 

  28. Umene K, Enquist LW (1981) A deletion analysis of lambda hybrid phage carrying the Us region of herpes simplex virus type 1 (Patton). I. Isolation of deletion derivatives and identification ofchi-likes sequences. Gene 13: 251–268

    Google Scholar 

  29. Umene K, Eto T, Mori R, Takagi Y, Enquist LW (1984) Herpes simplex virus type 1 restriction fragment polymorphism determined using Southern hybridization. Arch Virol 80: 275–290

    Google Scholar 

  30. Umene K, Watson RJ, Enquist LW (1984) Tandem repeated DNA in an intergenic region of herpes simplex virus type 1 (Patton). Gene 30: 33–39

    Google Scholar 

  31. Umene K (1985) Variability of the region of herpes simplex virus type 1 genome yielding defective DNA:SmaI fragment polymorphism. Intervirology 23: 131–139

    Google Scholar 

  32. Umene K, Enquist LW (1985) Isolation of novel herpes simplex virus type 1 derivatives with tandem duplications of DNA sequences encoding immediate-early mRNA-5 and an origin of replication. J Virol 53: 607–615

    Google Scholar 

  33. Umene K (1986) Conversion of a fraction of the unique sequence to part of the inverted repeats in the S component of the herpes simplex virus type 1 genome. J Gen Virol 67: 1035–1048

    Google Scholar 

  34. Umene K (1987) Restriction endonucleases recognizing DNA sequences of four base pairs facilitate differentiation of herpes simplex virus type 1 strains. Arch Virol 97: 197–214

    Google Scholar 

  35. Vlazny DA, Kwong A, Frenkel N (1982) Site-specific cleavage/packaging of herpes simplex virus DNA and the selective maturation of nucleocapsids containing full-length viral DNA. Proc Natl Acad Sci USA 79: 1423–1427

    Google Scholar 

  36. Watson RJ, Umene K, Enquist LW (1981) Reiterated sequences within the intron of an immediate-early gene of herpes simplex virus type 1. Nucleic Acids Res 9: 4189–4199

    Google Scholar 

  37. Watson RJ, Vande Woude GF (1982) DNA sequence of an immediate-early gene (IE mRNA-5) of herpes simplex virus type 1. Nucleic Acids Res 10: 979–991

    Google Scholar 

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Authors and Affiliations

  1. Department of Virology, Faculty of Medicine, Kyushu University, Fukuoka

    K. Umene

  2. Department of Dermatology, Faculty of Medicine, Kinki University, Osaka, Japan

    M. Yoshida

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  1. K. Umene
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  2. M. Yoshida
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Umene, K., Yoshida, M. Reiterated sequences of herpes simplex virus type 1 (HSV-1) genome can serve as physical markers for the differentiation of HSV-1 strains. Archives of Virology 106, 281–299 (1989). https://doi.org/10.1007/BF01313958

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  • DOI: https://doi.org/10.1007/BF01313958

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