Summary
The denaturation of poliovirus procapsids at pH 6.5 was studied, both in the frozen and liquid condition. Denaturation involved alteration of antigenic and physical features (isoelectric pH, alkali dissociability, sedimentation coefficient). Magnesium was a stabilizing factor, and the sedimentation coefficient was the most denaturation-sensitive feature.
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References
Hummeler K, Hamparian VV (1958) Studies on the complement fixing antigens of poliomyelitis. I. Demonstration of type and group specific antigens in native and heated viral preparations. J Immunol 81: 499–505
Marongiu ME, Pani A, Corrias MV, Sau M, La Colla P (1981) Poliovirus morphogenesis. I. Identification of 80 S dissociable particles and evidence for the artifactual production of procapsids. J Virol 39: 341–347
Onodera S, Phillips BA (1987) A novel method for obtaining poliovirus 14 S pentamers from procapsids and their self-assembly into virus-like shells. Virology 159: 278–287
Onodera S, Cardamone JJ, Phillips BA (1986) Biological activity and electron microscopy of poliovirus 14 S particles obtained from alkali-dissociated procapsids. J Virol 58: 610–618
Putnak JR, Phillips BA (1981) Picornaviral structure and assembly. Microbiol Rev 45: 287–315
Putnak JR, Phillips BA (1982) Poliovirus empty capsid morphogenesis: evidence for conformational differences between self- and extract-assembled empty capsids. J Virol 41: 792–800
Rombaut B, Vrijsen R, Boeyé A (1983) Epitope evolution in poliovirus maturation. Arch Virol 76: 289–298
Rombaut B, Vrijsen R, Boeyé A (1984) In vitro assembly of poliovirus empty capsids: antigenic consequences and immunological assay of the morphopoietic factor. Virology 135: 546–550
Rombaut B, Vrijsen R, Boeyé A (1985) Stabilization by host cell components and Mg2+ of the neutralization epitopes of poliovirus. J Gen Virol 66: 303–307
Rombaut B, Vrijsen R, Boeyé A (1986) Assembly factors in poliovirus morphogenesis. Virology 153: 137–144
Rombaut B, Vrijsen R, Boeyé A (1987) A pH-dependent dissociation of poliovirus procapsids. Virology 157: 245–247
Rombaut B, Vrijsen R, Brioen P, Boeyé A (1982) A pH-dependent antigenic conversion of empty capsids of poliovirus studied with the aid of monoclonal antibodies to N and H antigen. Virology 122: 215–218
Vrijsen R, Rombaut B, Boeyé A (1983) A simple quantitative protein A micro-immunoprecipitation method; assay of antibodies to the N and H antigens of poliovirus. J Immunol Methods 59: 217–220
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Rombaut, B., Vrijsen, R. & Boeyé, A. Denaturation of poliovirus procapsids. Archives of Virology 106, 213–220 (1989). https://doi.org/10.1007/BF01313954
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DOI: https://doi.org/10.1007/BF01313954