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Mechanisms of protective activity of 16,16-dimethyl PGE2 and acetazolamide on gastric and duodenal lesions in rats

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Abstract

16,16-Dimethyl PGE2 (16,16-dmPGE2), given orally at 10–30 μg/kg, had no effects on gastric acid secretion, or carbonic anhydrase activity, but did increase HCO3 − secretion in both the stomach and duodenum of rats. 16,16-dmPGE2, at nonantisecretory doses, potently inhibited indomethacin- and water-immersion stress-induced gastric lesions and mepirizole-induced duodenal lesions in rats. Acetazolamide, given orally at 50 mg/kg, markedly inhibited carbonic anhydrase activity, but had no effects on gastric acid secretion and the basal and 16,16-dmPGE2-stimulated HCO3 − secretion. Acetazolamide, at a nonantisecretory dose, had no effects on indomethacin-induced gastric lesions and mepirizole-induced duodenal lesions, but significantly inhibited water-immersion stress-induced gastric lesions. Combined administration of 16,16-dmPGE2 and acetazolamide did not influence the protective activity of 16,16-dmPGE2 on these lesions. The mechanism of the cytoprotective activity of 16,16-dmPGE2 may involve an increase in HCO3 − secretion (nonmediated by carbonic anhydrase), while mechanisms involved in the effects of acetazolamide are apparently different.

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Authors and Affiliations

  1. Department of Applied Pharmacology, Kyoto College of Pharmacy, Yamashina, 607, Kyoto, Japan

    Koji Takeuchi PhD, Hiroshi Ohtsuki MA & Susumu Okabe PhD

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  1. Koji Takeuchi PhD
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  2. Hiroshi Ohtsuki MA
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  3. Susumu Okabe PhD
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Takeuchi, K., Ohtsuki, H. & Okabe, S. Mechanisms of protective activity of 16,16-dimethyl PGE2 and acetazolamide on gastric and duodenal lesions in rats. Digest Dis Sci 31, 406–411 (1986). https://doi.org/10.1007/BF01311677

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  • DOI: https://doi.org/10.1007/BF01311677

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