Skip to main content
SpringerLink
Log in

Detection of polycistronic transcripts in Newcastle disease virus infected cells and identification of their sequence content

  • Original Papers
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

The synthesis of six to seven polycistronic transcripts of Newcastle disease virus (NDV) in BHK cells was detected by Northern hybridization using cDNA clones generated by reverse transcription of five NDV mRNAs. Within the molecular weight range resolved by the gel electrophoresis system employed, four of the transcripts were suggested to be distronic, containing sequences of two genes, NP-P, P-M, M-F0 and F0-HN, respectively. In addition, tricistronic molecules of M-F0-HN and possibly of NP-P-M as well as P-M-F0 appeared to develop, although they were very low in amount. These data suggest a gene order of NP-P-M-F0-HN on the NDV genome. The polycistronic as well as monocistronic transcripts were generated with an almost constant proportion in amount throughout the virus replication. Further, at least several of them were also generated under the conditions where only the primary transcription was allowed by inhibitingde novo protein synthesis. Therefore, it appears likely that there is no distinct temporal control in NDV genome expression.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Alwine JC, Kemp DJ, Stark GR (1977) Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci USA 74: 5350–5354

    Google Scholar 

  2. Aviv H, Leder P (1972) Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci USA 69: 1408–1412

    Google Scholar 

  3. Ishida N, Taira H, Omata T, Mizumoto K, Hattori S, Iwasaki K, Kawakita M (1986) Sequence of 2617 nucleotides from the 3′ end of Newcastle disease virus genome RNA and the predicted amino acid sequence of viral NP protein. Nucleic Acids Res 14: 6551–6564

    Google Scholar 

  4. Berger SL, Birkenmeier CS (1979) Inhibition of intractable nucleases with ribonucleoside-vanadyl complexes: isolation of messenger ribonucleic acid from resting lymphocytes. Biochemistry 18: 5143–5149

    Google Scholar 

  5. Blumberg B, Giorgi C, Rose K, Kolakofsky D (1985) Sequence determination of the Sendai virus fusion protein gene. J Gen Virol 66: 317–331

    Google Scholar 

  6. Bollum FJ (1968) Deoxynucleotide polymerizing enzymes from calf thymus gland. In:Grossman L, Moldave K (eds) Methods in enzymology, vol 12 B. Academic Press, New York, pp 591–611

    Google Scholar 

  7. Bratt MA, Hightower LE (1977) Genetics and paragenetic phenomena of paramyxoviruses. In:Frankel-Conrat H, Wagner RR (eds) Comprehensive virology, vol 9. Plenum, New York London, pp 457–523

    Google Scholar 

  8. Bratt MA, Robinson WS (1967) Ribonucleic acid synthesis in cells infected with Newcastle disease virus. J Mol Biol 23: 1–21

    Google Scholar 

  9. Carter MJ, Willcocks MM, ter Meulen V (1983) Defective translation of measles virus matrix protein in a subacute sclerosing panencephalitis cell line. Nature 305: 153–155

    Google Scholar 

  10. Choppin WP, Compans RW (1975) Reproduction of paramyxoviruses. In:Frankel-Conrat H, Wagner RR (eds) Comprehensive virology, vol 4. Plenum, New York London, pp 95–178

    Google Scholar 

  11. Chambers P, Miller NS, Bingham RW, Emmerson PT (1986) Molecular cloning of complementary DNA to Newcastle disease virus, and nucleotide sequence analysis of the junction between the genes encoding the haemagglutinin-neuraminidase and large protein. J Gen Virol 67: 475–486

    Google Scholar 

  12. Collins PL, Hightower LE, Ball A (1980) Transcriptional map for Newcastle disease virus. J Virol 35: 682–693

    Google Scholar 

  13. Collins PL, Wertz GW, Ball LA, Hightower LE (1982) Coding assignments of the five smaller messenger RNAs of Newcastle disease virus (NDV). J Virol 43: 1024–1031

    Google Scholar 

  14. Gupta KC, Kingsbury DW (1985) Polytranscripts of Sendai virus do not contain intervening polyadenylate sequences. Virology 141: 102–109

    Google Scholar 

  15. Hamaguchi M, Nishikawa K, Toyoda T, Yoshida T, Hanaichi T, Nagai Y (1985) Transcriptive complex of Newcastle disease virus: II. Structural and functional assembly associated with the cytoskeletal framework. Virology 147: 295–308

    Google Scholar 

  16. Hamaguchi M, Yoshida T, Nagai Y (1984) Protein kinase associated with Newcastle disease virus. Med Microbiol Immunol (Berl) 172: 223–231

    Google Scholar 

  17. Hamaguchi M, Yoshida T, Nishikawa K, Naruse H, Nagai Y (1983) Transcriptive complex of Newcastle disease virus: I. Both L and P proteins are required to constitute an active complex. Virology 128: 105–117

    Google Scholar 

  18. Hightower LE, Morrison TG, Bratt MA (1975) Relationships among the polypeptides of Newcastle disease virus. J Virol 16: 1599–1607

    Google Scholar 

  19. Hoeijmakers JHJ, Borst P, Van den Burg J, Weissmann C, Cross GAM (1980) The isolation of plasmids containing DNA complementary to messenger RNA for variant surface glycoproteins ofTrypanosoma brucei. Gene 8: 391–417

    Google Scholar 

  20. Laemmli UK (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T 4. Nature 227: 680–685

    Google Scholar 

  21. Loening UE, Jones KW, Birnstiel ML (1969) Properties of the ribosomal RNA precursor inXenopus laevis; comparison to the precursor in mammals and in plants. J Mol Biol 45: 353–366

    Google Scholar 

  22. Mackey JK, Brackmann KH, Green MR, Green M (1977) Preparation and characterization of heighly radioactivein vitro labelled adenovirus DNA restriction fragments. Biochemistry 16: 4478–4483

    Google Scholar 

  23. Mandel M, Higa A (1970) Calcium-dependent bacteriophage DNA infection. J Mol Biol 53: 159–162

    Google Scholar 

  24. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  25. Master PS, Samuel CE (1984) Detection ofin vivo synthesis of polycistronic mRNAs of vesicular stomatitis virus. Virology 134: 277–286

    Google Scholar 

  26. McMaster GK, Carmichael GG (1977) Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci USA 74: 4835–4838

    Google Scholar 

  27. Miller TJ, Stone HO (1981) Transcription of the Newcastle disease virus genomein vitro in a Hepes-buffered system. In:Bishop DHL, Compans RW (eds) The replication of negative-strand viruses. Elsevier-North Holland, New York, pp 493–502

    Google Scholar 

  28. Nagai Y, Klenk H-D, Rott R (1976) Proteolytic cleavage of the viral glycoproteins and its significance for the virulence of Newcastle disease virus. Virology 72: 494–508

    Google Scholar 

  29. Nishikawa K, Isomura S, Suzuki S, Watanabe E, Hamaguchi M, Yoshida T, Nagai Y (1983) Monoclonal antibodies to the HN glycoprotein of Newcastle disease virus. Biological characterization and use for strain comparisons. Virology 130: 318–330

    Google Scholar 

  30. Paterson R, Harris T, Lamb R (1984) Analysis and gene assignment of mRNAs of a paramyxovirus Simian virus 5. Virology 138: 310–323

    Google Scholar 

  31. Pelham HRB, Jacson RJ (1976) An efficient mRNA-dependent translation system from reticulocyte lyzates. Eur J Biochem 67: 247–256

    Google Scholar 

  32. Ricciardi RP, Miller JS, Roberts BE (1979) Purification and mapping of specific mRNAs by hybridization-selection and cell-free translation. Proc Natl Acad Sci USA 76: 4927–4931

    Google Scholar 

  33. Richardson C, Scheid A, Choppin PW (1980) Specific inhibition of paramyxovirus and myxovirus replication by oligopeptides with amino acid sequences similar to those at the N-termini of the F1 or HA2 viral polypeptides. Virology 105: 205–222

    Google Scholar 

  34. Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517

    Google Scholar 

  35. Spanier BB, Bratt MA (1977) The 50 S and 35 S RNAs from Newcastle disease virus-infected cells. J Gen Virol 35: 439–454

    Google Scholar 

  36. Udem S, Cook K (1984) Isolation and characterization of measles virus intracellular nucleocapsid RNA. J Virol 49: 57–65

    Google Scholar 

  37. Varich NL, Lukashevich IS, Kaverin NV (1979 a) Newcastle disease virus-specific RNA: an analysis of 24 S and 35 S transcripts. Acta Virol 23: 273–283

    Google Scholar 

  38. Varich NL, Lukashevich IS, Kaverin NV (1979 b) Newcastle virus-specific RNA: hybridization competition of the non-dissociable 35 S RNA with individual 18 S RNA species. Acta Virol 23: 341–343

    Google Scholar 

  39. Weiss SR, Bratt MA (1975) Effect of cordycepin (3'-deoxyadenosine) on virus-specific RNA species synthesized in Newcastle disease virus-infected cells. J Virol 16: 1575–1583

    Google Scholar 

  40. Wilde A, Morrison T (1984) Structural and functional characterization of Newcastle disease virus polycistronic RNA species. J Virol 51, 71–76

    Google Scholar 

  41. Wilde A, McQuain C, Morrison T (1986) Identification of the sequence content of four polycistronic transcripts synthesized in Newcastle disease virus infected cells. Virus Res 5: 77–95

    Google Scholar 

  42. Wreschner DH, James RH, Silverman RH, Kerr IM (1981) Ribosomal RNA cleavage, nuclease activation and 2-5 A (ppp(A 2'p)nA) in interferon-treated cells. Nucleic Acids Res 9: 1571–1581

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya, Japan

    T. Toyoda, M. Hamaguchi & Y. Nagai

Authors
  1. T. Toyoda
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Hamaguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Y. Nagai
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

With 6 Figures

Rights and permissions

Reprints and permissions

About this article

Cite this article

Toyoda, T., Hamaguchi, M. & Nagai, Y. Detection of polycistronic transcripts in Newcastle disease virus infected cells and identification of their sequence content. Archives of Virology 95, 97–110 (1987). https://doi.org/10.1007/BF01311337

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01311337

Keywords

Search

Navigation