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Expression at the cell surface of native fusion protein of the Newcastle disease virus (NDV) strain Italien from cloned cDNA

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Summary

A cDNA library was constructed with poly(A)+-mRNAs from NDV-Italien infected BHK-21 cells. A clone, that hybridized to the F gene mRNA, was sequenced. A long open reading frame encodes for a protein of 553 amino acids, with a calculated molecular weight of 59,153, consisting of twelve cysteine residues and six potential glycosylation sites. The protein sequence contains a hydrophobic region at the N-terminus of F1 and a presumptive long transmembrane fragment near the C-terminus.

Comparison of the F proteins from NDV strains Italien and Australia-Victoria shows that the sequences are very similar, with conservation of most cysteine residues and of the potential glycosylation sites.

The F coding sequence was inserted into the genome of vaccinia virus under the control of vaccinia P7.5 transcriptional regulatory sequences. Expression of F protein was demonstrated by indirect immunofluorescence with five anti-F monoclonal antibodies known to react with conformational epitopes.

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Author notes

  1. S. de Henau

    Present address: Department of Molecular Genetics, Smith Kline-RIT, 1330, Rixensart, Belgium

Authors and Affiliations

  1. Laboratory of Biological Chemistry, Free University of Brussels, ULB, Rhode-St-Genèse

    D. Espion, S. de Henau, C. Letellier, C. D. Wemers & A. Burny

  2. Laboratory of Macromolecules at Interface, Free University of Brussels, ULB, Brussels, Belgium

    R. Brasseur

  3. Smith Kline & French Laboratories, Philadelphia, Pennsylvania, USA

    J. F. Young, M. Gross & M. Rosenberg

  4. National Institute for Veterinary Research, Brussels

    G. Meulemans

  5. Faculty of Agronomy, Gembloux, Belgium

    A. Burny

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  1. D. Espion
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Espion, D., de Henau, S., Letellier, C. et al. Expression at the cell surface of native fusion protein of the Newcastle disease virus (NDV) strain Italien from cloned cDNA. Archives of Virology 95, 79–95 (1987). https://doi.org/10.1007/BF01311336

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  • DOI: https://doi.org/10.1007/BF01311336

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