Summary
Horse erythrocytes treated with equine infectious anemia virus hemagglutinin were found to be lysed after incubation with fresh horse serum at 37° C. Fresh guinea pig serum induced more efficient hemolysis than horse serum. Direct immunofluorescence test revealed the adsorption of complement factors on the surface of the erythrocytes. Calcium and magnesium ions were necessary for the hemolysis to take place. Antibody against equine infectious anemia virus enhanced the virus-induced complement-mediated hemolysis. These observations indicated that the classical pathway of complement activation was responsible for this virus-induced hemolysis and suggest the possibility that virus antigen, anti-viral antibody and complement may play an important role in the genesis of the anemia of horses infected with the equine infectious anemia virus.
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References
Banks KL, McGuire TC (1975) Surface receptors on neutrophils and monocytes from immunodeficient and normal horses. Immunology 28: 581–588
Brown EJ, Frank MM (1981) Complement activation. Immunol Today 2: 129–134
Brown EJ, Joiner KA, Frank MM (1984) Complement. In:Paul WE (ed) Fundamental immunology. Raven Press, New York, pp 645–668
Henson JB, McGuire TC (1974) Equine infectious anemia. Prog Med Virol 18: 143–159
Ishii S (1963) Equine infectious anemia or swamp fever. Adv Vet Sci 8: 263–298
Issel CJ, Coggins L (1979) Equine infectious anemia: current knowledge. J Am Vet Med Ass 174: 727–733
Kai C, Yoshikawa Y, Yamanouchi K, Okada H (1983) Isolation and identification of the third component of complement of Japanese quails. J Immunol 130: 2814–2820
Kawamura Jr A (1977) Preparation of materials. In:Kawamura Jr A (ed) Fluorescent antibody techniques and their applications, 2nd ed. Univ Tokyo Press, Tokyo, chapt 3, pp 13–76
Kono Y (1969) Viremia and immunological responses in horses infected with equine infectious anemia virus. Natl Inst Anim Health Q 9: 1–9
Kono Y, Kobayashi K (1967) Specificity of assay of equine infectious anemia virus in horse leukocyte culture. Natl Inst Anim Health Q 7: 138–144
Kono Y, Kobayashi K, Fukunaga Y (1971) Distribution of equine infectious anemia virus in horses infected with the virus. Natl Inst Anim Health Q 11: 11–20
Kono Y, Kobayashi K, Fukunaga Y (1971) Serological comparison among various strains of equine infectious anemia virus. Arch Ges Virusforsch 34: 202–208
McGuire TC, Crawford TB (1979) Immunology of a persistent retrovirus infection-Equine infectious anemia. Adv Vet Sci Comp Med 23: 137–159
McGuire TC, Crawford TB, Henson JB (1971) Immunofluorescent localization of equine infectious anemia virus in tissue. Am J Pathol 62: 283–292
McGuire TC, Crawford TB, Henson JB (1972) Equine infectious anemia: Detection of infectious virus-antibody complexes in the serum. Immunol Commun 1: 545–551
McGuire TC, Henson JB, Bueger D (1969) Complement (C 3)-coated red blood cells following infection with the virus of equine infectious anemia. J Immunol 103: 293–299
McGuire TC, Henson JB, Quist SE (1969) Impaired bone marrow response in equine infectious anemia. Am J Vet Res 30: 2099–2104
McGuire TC, Henson JB, Quist SE (1969) Viral-induced hemolysis in equine infectious anemia. Am J Vet Res 30: 2091–2097
Obara J, Nakajima H (1961) Life span of 51 Cr-labeled erythrocytes in equine infectious anemia. Jpn J Vet Sci 23: 207–210
Obara J, Nakajima H (1961) Iron metabolism in equine infectious anemia. V. Measurement of sites on erythrocyte destruction. Natl Inst Anim Health Q 1: 32–35
Ouchterlony Ö, Nilsson LÅ (1973) Immunodiffusion and immunoelectrophoresis. In:Weir DM (ed) Immunochemistry, 2nd ed. Blackwell Scientific Publications, Oxford (Handbook of experimental immunology, vol 1, pp 19.1–19.39)
Perryman LE, McGuire TC, Banks KL, Henson JB (1971) Decreased C 3 levels in a chronic virus infection: equine infectious anemia. J Immunol 106: 1074–1078
Schmid RW, Reilley CN (1957) New complexon for titration of calcium in the presence of magnesium. Anal Chem 29: 264–268
Sentsui H, Kono Y (1976) Hemagglutination by equine infectious anemia virus. Infect Immun 14: 325–331
Sentsui H, Kono Y (1978) Preparation of hemagglutinating antigen of equine infectious anemia virus from infected equine leukocyte cultures. Natl Inst Anim Health Q 18: 39–40
Sentsui H, Kono Y (1981) Hemagglutination by several strains of equine infectious anemia virus. Arch Virol 67: 75–84
Sentsui H, Kono Y (1981) Hemagglutination-inhibition tests with different strains of equine infectious anemia virus. Am J Vet Res 42: 1949–1952
Sentsui H, Kono Y (1987) Phagocytosis of horse erythrocytes treated with equine infectious anemia virus by cultivated horse leukocytes. Arch Virol 95: 67–77
Shurin SB, Stossel TP (1978) Complement (C 3)-activated phagocytosis by lung macrophages. J Immunol 120: 1305–1312
Yamamoto H, Konno S (1967) Pathological studies on bone marrow in equine infectious anemia. I. Macroscopical findings on whole longitudinal sections of bone marrow. Natl Inst Anim Health Q 7: 40–53
Yamamoto H, Konno S (1967) Pathological studies on bone marrow in equine infectious anemia. II. Histopathology of vertebral, sternal and femoral bone marrow. Natl Inst Anim Health Q 7: 84–90
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Sentsui, H., Kono, Y. Complement-mediated hemolysis of horse erythrocytes treated with equine infectious anemia virus. Archives of Virology 95, 53–66 (1987). https://doi.org/10.1007/BF01311334
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DOI: https://doi.org/10.1007/BF01311334