Summary
Persistent measles virus infection of human HEp-2 or L-41 cells was accompanied by pronounced structural and functional changes of isolated intracellular viral nucleocapsids (NCs). The bulk of persistent NCs possessed altered conformation and a “string-of-beads” appearance, contained substantial amounts of subgenomic size RNAs, exhibited reduced transcriptase activityin vitro and lacked infectivity on transfection of susceptible cells. Immunogold staining revealed negligible binding of anti-P protein monoclonal antibodies to the “string-of-beads” type NCs, thus suggesting their non-functional state.
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Andzhaparidze, O.G., Chaplygina, N.M., Bogomolova, N.N. et al. Non-infectious morphologically altered nucleocapsids of measles virus from persistently infected cells. Archives of Virology 95, 17–28 (1987). https://doi.org/10.1007/BF01311331
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DOI: https://doi.org/10.1007/BF01311331