Summary
The efficiency of transfection of purified HCMV genome was studied in synchronized human embryo fibroblasts.
The data show that HCMV DNA infection performed in S phase is more efficient than in cells infected in G2, M and G1 phases of cell cycle, respectively.
These findings stress the importance of the metabolic state of cell cultures in HCMV genome transfection.
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Musiani, M., Zerbini, M. Influence of cell cycle on the efficiency of transfection with purified human cytomegalovirus DNA. Archives of Virology 78, 287–292 (1983). https://doi.org/10.1007/BF01311323
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DOI: https://doi.org/10.1007/BF01311323