Summary
Rabbit antisera to the stump-tailed macaque polyomavirus (STMV) which had been shown by immunoelectron microscopy and indirect immunofluorescence to react with the polyomavirus found in FRhK-4 cells (FRKV), also gave precipitin lines in counter-immunoelectrophoresis (CIE) and double diffusion in gel (GD) when reacted with FRKV. The reactions in GD showed identity with that of a rabbit antiserum to FRKV.
Naturally occurring antibody to FRKV (anti-FRKV) was found by CIE in 48 per cent of 353 cattle, 1/106 pigs and 1/20 goats but not in any of 13 other species including 45 rhesus monkeys and 97 humans. Each of 9 anti-FRKV positive samples from cattle, the goat serum, but not the pig serum gave a line of identity with the rabbit antiserum to FRKV in GD against FRKV. Detection of anti-FRKV in colostrum deprived newborn calves and in commercial foetal calf sera (FBS) indicates that intra-uterine infection of cattle with FRKV may occur.
FRKV adapted readily to growth in secondary calf kidney cultures and grew more rapidly and to higher titres than in the FRhK-4 cultures.
We conclude that FRKV is probably another strain of STMV and that the natural hosts of these viruses are cattle and not primates. Evidence of intrauterine infection of cattle implies that infectious FRKV may be present in some FBS and may thus have gained entry into various susceptible cell lines, particularly primate kidney.
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Parry, J.V., Lucas, M.H., Richmond, J.E. et al. Evidence for a bovine origin of the polyomaviurs detected in foetal rhesus monkey kidney cells, FRhK-4 and -6. Archives of Virology 78, 151–165 (1983). https://doi.org/10.1007/BF01311311
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DOI: https://doi.org/10.1007/BF01311311