Skip to main content
Log in

pH-dependent fusion of tick-borne encephalitis virus with artificial membranes

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

pH-dependent fusion of TBE virus with artificial membranes was effective at slightly acidic pH with maximum at 6.4. The influence of various changes in E protein conformation on fusion process was studied.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Clarke DH, Casals G (1985) Techniques for haemagglutination and haemagglutination-inhibition with arthroped-borne viruses. Am J Trop Med Hyg 7: 561–573

    Google Scholar 

  2. Gollins SW, Porterfield JS (1985) Flavivirus infection enhancement in macrophages: an electron microscopic study of viral cellular entry. J Gen Virol 66: 1969–1982

    Google Scholar 

  3. Gollins SW, Porterfield JS (1986) A new mechanism for the neutralization of enveloped virus by antiviral antibody. Nature 321: 244–246

    Google Scholar 

  4. Gollins SW, Porterfield JS (1986) pH-dependent fusion between the flavivirus West Nile and liposomal model membranes. J Gen Virol 67: 157–166

    Google Scholar 

  5. Gollins SW, Porterfield JS (1986) The uncoating and infectivity of the flavivirus West Nile on interaction with cells: effects of pH and ammonium chloride. J Gen Virol 67: 1941–1950

    Google Scholar 

  6. Guirakhoo F, Heinz FX, Kunz C (1989) Epitope model of tickborne encephalitis virus envelope glycoprotein E: analysis of structural properties, role of carbohydrate side chain and conformational changes occurring at acidic pH. Virology 169: 90–99

    Google Scholar 

  7. Hase T, Summers PL, Eckels KH (1989) Flavivirus entry into cultured mosquito cells and Ruman peripheral blood monocytes. Arch Virol 104: 129–143

    Google Scholar 

  8. Hase T, Summers PL, Cohen WH (1989) A comparative study of entry modes into C 6/36 cells by Semliki Forest and Japanese encephalitis viruses. Arch Virol 108: 101–114

    Google Scholar 

  9. Heinz FX, Berger R, Tuma W, Kunz C (1983) A topological and functional model of epitopes on the structural glycoprotein of tick-borne encephalitis virus defined by monoclonal antibodies. Virology 126: 525–537

    Google Scholar 

  10. Heinz FX (1986) Epitope mapping of flavivirus glycoproteins. Adv Virus Res 31: 1003–168

    Google Scholar 

  11. Kimura T, Gollins SW, Porterfield JS (1986) The effect of pH on the early interaction of West Nile virus with p388 D 1 cells. J Gen Virol 67: 2423–2433

    Google Scholar 

  12. Kimura T, Ohyama A (1988) Association between the pH dependent conformational changes of West Nile flavivirus E protein and virus-mediated membrane fusion. J Gen Virol 69: 1247–1254

    Google Scholar 

  13. Kushch AA, Novak M, Melnikova YF, Gaidamovich SY, Gresikova M, Sekeyova M, Novokhatsky AS, Mikheeva TG, Sveshnikova NA, Borecky LS, Zhdanov VM (1986) Preparation and characterization of hybridomas secreting monoclonal antibodies to tick-borne encephalitis virus. Acta Virol 30: 199–205

    Google Scholar 

  14. Mandl CW, Guirakhoo F, Holzmann H, Heinz FX, Kunz C (1989) Antigenic structure of the flavivirus envelope protein E at the molecular level using tick-borne encephalitis virus as a model. J Virol 63: 564–571

    Google Scholar 

  15. Roerig JT, Hunt AR, Johnson AJ, Hawkes RA (1989) Synthetic peptides derived from the deduced amino acid sequence of the E glycoprotein of Murray Valley encephaltitis virus elicit antiviral antibody. Virology 171: 46–60

    Google Scholar 

  16. Summers RL, Cohen WH, Ruiz MM, Itase T, Eckels KH (1989) Flaviviruses can mediate fusion from without inAedes albopictus mosquito cell cultures. Virus Res 12: 383–392

    Google Scholar 

  17. Timofeev AV, Elbert LB, Terletskaya EN, Maldov DG, Karavanov AS (1987) Use of direct solid-phase enzyme immunoassay for the evaluation of the immunological activity of tick-borne encephalitis vaccine. J Microbiol Epidemiol Immunol 9: 86–93 (in Russian)

    Google Scholar 

  18. Timofeev AV, Kushch AA, Vorovitch MF, Tugizov SM, Elbert LB, Lvov DK (1990) A study of NS 3 nonstructural protein of tick-borne encephalitis virus using monoclonal antibodies against the virus. Arch Virol [Suppl 1]: 119–124

    Google Scholar 

  19. Vorovitch MF, Timofeev AV, Maldov DG, Khaustov VI, Elbert LB (1989) Alterations of biological properties of tick-borne encephalitis virus after disruption of disulfide bonds in E protein. Voprosy Virusologii 6: 698–701 (in Russian)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vorovitch, M.F., Timofeev, A.V., Atanadze, S.N. et al. pH-dependent fusion of tick-borne encephalitis virus with artificial membranes. Archives of Virology 118, 133–138 (1991). https://doi.org/10.1007/BF01311309

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01311309

Keywords

Navigation