Summary
In our previous study we found that the ARH 77 human B lymphoblastoid cell line, originating from a patient with multiple myeloma, produced both human interferon-α (HuIFN-α) and HuIFN-β after induction with Sendai virus. In order to examine whether IFN-α-producing ARH 77 cell clones can be separated from IFN-β-producing ones, the ARH 77 line was cloned by the soft agar method. Twelve clones chosen at random were examined for IFN production and the antigenic types of IFN produced were determined. All examined clones simultaneously produced both HuIFN-α and HuIFN-β, although the ratio of HuIFN-α to HuIFN-β production was variable among the clones. This result suggests that one lymphoblastoid cell can produce both HuIFN-α and HuIFN-β.
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Hoshino, S., Imanishi, J., Matsuoka, H. et al. The production of interferon-α and -β by cloned human lymphoblastoid cells. Archives of Virology 76, 355–358 (1983). https://doi.org/10.1007/BF01311202
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DOI: https://doi.org/10.1007/BF01311202