Summary
Adenovirus 8 (Ad8, strain 1127) has the general structural features of an adenovirus; it produces the genus-specific hexon antigen. The fiber length of 12 nm corresponds to that of other subgenus D adenoviruses. In hemagglutination-inhibition, Ad8 is related to Ad9 and Ad15/H9. The slow multiplication, compared to most other subgenus D viruses, was shown not to be caused by an extended growth cycle, but appears to be due to an inefficient virus release into the medium.
After restriction analysis with the enzymes Sma I and Bgl II, the typical fragment patterns of species from subgenus D were obtained, while very different patterns emerged from the analysis with Hind III, Bam HI, and Bst E II endonucleases.
Several strains of Ad8 produced more penton-associated toxin than Ad15/H9 used as reference virus from subgenus D, which could explain the “clumping CPE” caused by Ad8. Ad8 toxin was neutralized by antisera from many adenovirus species.
The poor virus yield of Ad8 was not improved by removing soluble viral or cellular substances; all other attempts to improve virus yield by varying the conditions of cell culture also failed.
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De Jong, J. C., Wigand, R., Kidd, A. H., Wadell, G., Kapsenberg, J. G., Muzerie, C. J., Wermenbol, A. G., Firtzlaff, R.-G.: Candidate adenovirus 40 and 41: fastidious adenovirus from human infant stool. J. Med. Virol.11, 215–231 (1983).
Doerfler, W.: Nonproductive infection of baby hmaster kidney cells (BHK21) with adenovirus type 12. Virology38, 587–606 (1969).
Döring, N., Nguyen, C. X., Wigand, R.: Neutralization of adenovirus toxins: Specificity and antigenic relationships. Med. Microbiol. Immunol.157, 325–334 (1972).
Everett, S. F., Ginsberg, H. S.: A toxinlike material separable from type 5 adenovirus particles. Virology6, 770–771 (1958).
Gelderblom, H., Bauer, H., Frank, H., Wigand, R.: The structure of group II adenoviruses. J. gen. Virol.1, 553–560 (1967).
Gelderblom, H., Maichle-Lauppe, I.: The fibers of fowl adenoviruses. Arch. Virol.72, 289–298 (1982).
Golden, B., McKee, A. P.: Enhancement of the infectivity titer of adenovirus type 8. Arch. Ophthalmol.83, 455–457 (1970).
Green, M., Mackey, J. K., Wold, W. S. M., Rigden, P.: Thirty-one human adenovirus serotypes (Ad 1–31) from five groups (A–E) based upon DNA genome homologies. Virology93, 481–492 (1979).
Green, M., Piña, M., Kimes, R. C.: Biochemical studies on adenovirus multiplication. XII. Plaqueing efficiencies of purified human adenoviruses. Virology31, 562–565 (1967).
Hanna, L., Jawetz, E.: Attempts to enhance infectivity of adenovirus type 8 for cell cultures. Proc. Soc. Exp. Biol.110, 707–709 (1962).
Horne, R. W., Brenner, S., Waterson, A. P., Wildy, P.: The icosaedral form of an adenovirus. J. Mol. Biol.1, 84–86 (1959).
Jawetz, E., Kimura, S., Nicholas, A. N., Thygeson, P., Hanna, L.: New type of APC virus from epidemic keratoconjunctivitis. Science122, 1190–1192 (1955).
Kellenberger, E., Arber, W.: Electron microscopical studies of phage multiplication. I. A method for quantitative analysis of particle suspensions. Virology3, 245–255 (1957).
Kron, I., Buchholz, M., Schulz, R., Quintenz, M., Wigand, R.: Laboratoriums-praxis bei Adenoviren. I. Qualitative und quantitative Immunofluoreszenz. Zbl. Bakt. Hyg., I. Abt. Orig.A 229, 159–170 (1974).
Lengyel, A., Nász, I.: Soluble components of adenovirus type 8. J. Virol.6, 406–413 (1970).
Norrby, E.: Comparative studies on the soluble components of adenovirus types 9 and 15 and the intermediate strain 9–15. J. Virol.2, 1200–1210 (1968).
Pereira, H. G., Kelly, B.: Dose-response curves of toxic and infective actions of adenovirus in HeLa cell cultures. J. gen. Microbiol.17, 517–524 (1957).
Pereira, H. G., Valentine, R. C.: Infectivity titrations and particle counts of adenovirus type 5. J. gen. Microbiol.19, 178–181 (1958).
Piña, M., Green, M.: Biochemical studies on adenovirus multiplication. IX. Chemical and base composition analysis of 28 human adenoviruses. Proc. Nat. Acad. Sci. U.S.A.54, 547–551 (1965).
Rosen, L.: A hemagglutination-inhibition technique for typing of adenoviruses. Am. J. Hyg.71, 120–128 (1960).
Selzer, B., Wigand, R.: Laboratoriumspraxis bei Adenoviren. VII. Empfänglichkeit von humanen diploiden Fibroblasten. Zbl. Bakt. Hyg., I. Abt. Orig.A 249, 287–295 (1981).
Wadell, G., de Jong, J. C.: Restriction endonucleases in identification of a genome type of adenovirus 19 associated with keratoconjunctivitis. Infect. Immun.27, 292–296 (1980).
Wadell, G., Hammarskjöld, M.-L., Winberg, G., Varsanyi, T. W., Sundell, G.: Genetic variability of adenoviruses. Ann. N. Y. Acad. Sci.354, 16–42 (1980).
Wigand, R.: Serologisch intermediäre Adenovirusstämme. Arch. ges. Virusforsch.17, 611–618 (1965).
Wigand, R., Bartha, A., Dreizin, R. S., Esche, H., Ginsberg, H. S., Green, M., Hierholzer, J. C., Kalter, S. S., McFerran, J. B., Petterson, U., Russell, W. C., Wadell, G.: Adenoviridae: Second Report. Intervirology18, 169–176 (1982).
Wigand, R., Bauer, H.: Über den cytopathogenen Effekt der Adenoviren des Menschen. Arch. ges. Virusforsch.14, 674–682 (1964).
Wigand, R., Gelderblom, H., Kron, I., Liem, I. T. F.: Structure, hemagglutinins and subgroup position of adenovirus 20, 25 and 28. Arch. ges. Virusforsch.38, 167–176 (1972).
Wigand, R., Keller, D.: Laboratoriumspraxis bei Adenoviren. III. Empfindlichkeit und Spezifität serologischer Untersuchungen. Zbl. Bakt. Hyg., I. Abt. Orig.A 234, 417–433 (1976).
Wigand, R., Keller, D., Werling, I.: Immunological relationship among human adenoviruses of subgenus D. Arch. Virol.72, 199–209 (1982).
Wigand, R., Schulz, R.: Laboratoriumspraxis bei Adenoviren. II. Empfindlichkeit verschiedener Zellkulturen bei Endpunkttitration. Zbl. Bakt. Hyg., I. Abt. Orig.A 231, 31–41 (1975).
Wigand, R., Wunn, W.: Die Vermehrung von Adenoviren der Gruppe II in HeLa-Zellen. Arch. ges. Virusforsch.21, 1–10 (1967).
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Wigand, R., Gelderblom, H., Özel, M. et al. Characteristics of mastadenovirus h8, the causative agent of epidemic keratoconjunctivitis. Archives of Virology 76, 307–319 (1983). https://doi.org/10.1007/BF01311198
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DOI: https://doi.org/10.1007/BF01311198