Summary
Immunoblotting with monoclonal as well as polyclonal lamin antibodies revealed that nuclear lamina proteins of human foreskin fibroblasts (HFF) were differentially affected after infection with human cytomegalovirus (HCMV). Lamin A immunoreactivity was progressively lost during the course of the infectious cycle whereas that of lamin C was comparatively stable. This process was not observed in herpes simplex virus-infected HFF. On the other hand, noninfected arrested HFF stimulated by serum to enter S-phase also exhibited loss of lamin A immunoreactivity. Selective in vivo proteolysis of lamin A is suggested as the possible underlying mechanism.
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References
Albrecht TH, Cavallo T, Cole NL, Graves K (1980) Cytomegalovirus: Development and progression of cytopathic effects in human cell culture. Lab Invest 42: 1–7
Benko D, Haltiwanger R, Hart G, Gibson W (1988) Virion basic phosphoprotein from human cytomegalovirus contains O-linked N-acetylglucosamine. Proc Natl Acad Sci USA 85: 2573–2577
Bibor-Hardy V, Suh M, Pouchelet M, Simard R (1982) Modifications of the nuclear envelope of BHK cells after infection with herpes simplex virus type 1. J Gen Virol 63: 81–94
Britt W, Vugler L, Stephens E (1988) Induction of complement-dependent and -independent neutralizing antibodies by recombinant-derived human cytomegalovirus gp 55–116 (gB). J Virol 62: 3309–3318
Davis L, Blobel G (1987) Nuclear pore complex contains a family of glycoproteins that includes p 62: glycosylation through a previously unidentified cellular pathway. Proc Natl Acad Sci USA 84: 7552–7556
Gerace L, Blobel G (1980) The nuclear envelope lamina is reversibly depolymerized during mitosis. Cell 19: 277–287
Hart G, Holt G, Haltiwanger R (1988) Nuclear and cytoplasmic glycosylation: novel saccharide linkages in unexpected places. TIBS 13: 380–384
Hirai K, Watanabe Y (1976) Induction of alpha-type DNA polymerase in human cytomegalovirus-infected Wi-38 cells. Biochim Biophys Acta 447: 328–339
Högner D (1986) Produktion monoklonaler Antikörper gegen Lamine und andere karyophile Proteine und Charakterisierung der Antigene. PhD Thesis, University of Giessen, Giessen, Federal Republic of Germany
Jahn G, Kouzarides T, Mach M, Scholl B-C, Plachter B, Traupe B, Preddie E, Satchwell S, Fleckenstein B, Barrell B (1987) Map position and nucleotide sequence of the gene for the large structural phosphoprotein of human cytomegalovirus. J Virol 61: 1358–1367
Kaiser CJ, Radsak K (1987) Inhibition by monensin of human cytomegalovirus DNA replication. Arch Virol 94: 229–245
Kari B, Lussenhop N, Goertz R, Wabuke-Bunoti M, Radeke R, Gehrz R (1986) Characterization of monoclonal antibodies reactive to several biochemically distinct human cytomegalovirus glycoprotein complexes. J Virol 60: 345–352
Lin J-C, Pagano J (1986) Sequential detection of different antigens induced by Epstein-Barr virus and herpes simplex virus in the same Western blot by using dual antibody probes. J Virol 59: 522–524
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275
Musiani M, Zerbini M (1984) Influence of cell cycle on the efficiency of transfection with purified human cytomegalovirus DNA. Arch Virol 78: 287–292
Newport J, Forbes D (1987) The nucleus: structure, function, and dynamics. Ann Rev Biochem 56: 535–565
Ottaviano Y, Gerace L (1985) Phosphorylation of the nuclear lamins during interphase and mitosis. J Biol Chem 260: 624–632
Paine P, Moore L, Horowitz S (1975) Nuclear envelope permeability. Nature 254: 109–114
Radsak K, Kaiser CJ, Haustein D, Rapp F (1988) Polypeptide synthesis in human fibroblasts infected with DNA-negative mutants of cytomegalovirus. Intervirology 29: 101–107
Radsak K, Wiegandt H, Unterdörfer U, Wagner C, Kaiser CJ (1985) Sodium butyrate selectively inhibits host cell glycoprotein synthesis in human fibroblasts infected with cytomegalovirus. Biosci Rep 5: 589–599
Severi B, Landini M, Govoni E (1988) Human cytomegalovirus morphogenesis: an ultrastructural study of the late cytoplasmic phases. Arch Virol 98: 51–64
Smith J (1980) An additional role for the outer nuclear membrane in the morphogenesis of herpes simplex virus. Intervirology 13: 312–316
Weiland F, Keil G, Reddehase M, Koszinowski U (1986) Studies on the morphogenesis of murine cytomegalovirus. Intervirology 26: 192–201
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Radsak, K., Schneider, D., Jost, E. et al. Alteration of nuclear lamina protein in human fibroblasts infected with cytomegalovirus (HCMV). Archives of Virology 105, 103–112 (1989). https://doi.org/10.1007/BF01311120
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DOI: https://doi.org/10.1007/BF01311120