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Interprotein disulfide bonding between F and G glycoproteins of human respiratory syncytial virus

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Summary

The envelope glycoprotein G, of human respiratory virus was purified by immunoaffinity chromatography using a monoclonal antibody reacting with G glycoprotein. The purified material was analyzed for its protein patterns and by western blot for its reactivity with specific monoclonal antibodies. In addition to the G specific proteins at 90 and 55 kilodalton (kDa) range, high molecular weight species were coeluted with G protein. Three high molecular weight species were noticed: one (140 kDa) reacting with fusion protein (F) monoclonal antibody and two other species (230 and 195 kDa) reacting with both fusion protein and G protein monoclonal antibodies. The protein reacting only with F monoclonal antibody consists of fusion protein dimer. Western blot and two dimensional gel electrophoretic analysis revealed that each of the other two complexes is composed of two moles of F protein and one mole of G protein. These two complexes differ in their molecular sizes depending on whether G is in the form of 90 or 55 kDa. Upon heat denaturation, fusion protein monomer (70 kDa) is released from the complex, leaving the two complexes, consisting of one mole of F protein and one mole of G protein (160 and 125 kDa species respectively). Disulfide-reducing agents are required to break the monomers of F and G complexes. These results provide a direct evidence for the presence of envelope glycoprotein complexes linked by interprotein disulfide bonding. This may have implications on the structural and functional properties of envelope glycoproteins.

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Authors and Affiliations

  1. Virology Research, Praxis Biologics, Rochester, New York, U.S.A.

    R. G. Arumugham, S. W. Hildreth & P. R. Paradiso

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  1. R. G. Arumugham
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  2. S. W. Hildreth
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  3. P. R. Paradiso
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Arumugham, R.G., Hildreth, S.W. & Paradiso, P.R. Interprotein disulfide bonding between F and G glycoproteins of human respiratory syncytial virus. Archives of Virology 105, 65–79 (1989). https://doi.org/10.1007/BF01311117

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  • DOI: https://doi.org/10.1007/BF01311117

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