Summary
A highly virulent mouse-adapted variant of influenza virus A/Aichi/2/68 (H3N2) was crossed either with the original A/USSR/90/77 (H1N1) influenza virus strain or with its mouse-adapted, moderately mouse virulent variant. The reassortants were characterized with respect to their genetic content and pneumovirulence for mice. The reassortants fell into three categories: avirulent, highly virulent (resembling in this respect the parent A/Aichi/2/68 virus) and moderately virulent (resembling the mouse-adapted A/USSR/90/77 parent virus). The analysis of the parental origin of the genes of 6 reassortants allowed to suggest that changes in the HA gene and in a polymerase gene (most likely, PB1) were necessary for the acquisition of virulence by the A/USSR/90/77 virus in the course of adaptation to mice, whereas the changes in two other polymerase genes as well as in the genes NA and NS were not involved. The low degree of pathogenicity characteristic of the mouse-adapted A/USSR/90/77 virus was determined by gene(s) other than HA.
Similar content being viewed by others
References
Bergelson LD, Bukrinskaya AG, Provkazova NV, Shaposhnikova GI, Kocharov SL, Shevchenko VP, Kornilaeva GV, Fomina-Ageeva EV (1982) Receptor determinants of human and animal influenza virus isolates; differences in receptor specificity of the H3 hemagglutinin based on species of origin. Eur J Biochem 128: 467–474
Ghendon YZ, Klimov AI, Ginzburg VP (1984) Studies of a recombinant which inherited the hemagglutinin from the human influenza virus A/Hong Kong/1/68 (H3N2) and other genes from influenza A/Duck/Ukraine/1/63 (H3N8). J Gen Virol 65: 165–172
Gitelman AK, Kaverin NV, Kharitonenkov IG, Rudneva IA, Zhdanov VM (1984) Changes in the antigenic specificity of influenza hemagglutinin in the course of adaptation to mice. Virology 134: 230–232
Gitelman AK, Kaverin NV, Kharitonenkov IG, Rudneva IA, Sklyanskaya EI, Zhdanov VM (1986) Dissociation of the haemagglutination inhibition and the infectivity neutralization in the reactions of influenza A/USSR/90/77 (H1N1) virus variants with monoclonal antibodies. J Gen Virol 67: 2247–2251
Kawaoka Y, Naeve CW, Webster RG (1984) Is virulence of H5N2 influenza viruses in chickens associated with loss of carbohydrate from the hemagglutinin? Virology 139: 303–316
Naeve CW, Hinshaw VS, Webster RG (1984) Mutations in the hemagglutinin receptor-binding site can change the biological properties of an infuenza virus. J Virol 128: 331–340
Rogers GN, Paulson JC, Daniels RS, Skehel JJ, Wilson JA, Wiley DC (1983) Single amino acid substitutions in influenza hemagglutinin change receptor binding specificity. Nature 304: 76–78
Rott R, Orlich M, Klenk H-D, Wang ML, Skehel JJ, Wiley DC (1984) Studies on the adaptation of influenza viruses to MDCK cells. EMBO J 3: 3329–3332
Rudneva IA, Kaverin NV, Varich NL, Gitelman AK, Makhov AM, Klimenko SM, Zhadnov VM (1986) Studies on the genetic determinants of influenza virus pathogenicity for mice with the use of reassortants between mouse-adapted and non-adapted variants of the same virus strain. Arch Virol 90: 237–248
Scholtissek C, Rott R, Orlich M, Harms E, Rohde W (1977) Correlation of pathogenicity and gene constellation of an influenza virus (fowl plague) I. Exchange of a single gene. Virology 81: 74–80
Schulman JL, Palese P (1976) Selection and identification of influenza virus recombinants of defined genetic composition. J Virol 20: 248–254
Schulman JL (1983) Virus-determined differences in the pathogenesis of influenza virus infections. In: Palese P, Kingsbury DW (eds) Genetics of influenza viruses. Springer, Wien New York, pp 303–320
Snyder MH, Buckler-White AJ, London WT, Tierney EL, Murphy BR (1987) The avian influenza virus nucleoprotein gene and a specific constellation of avian and human virus polymerase genes each specify attenuation of avian-human influenza A/Pintail/79 reassortant viruses for monkeys. J Virol 61: 2857–2863
Zhdanov VM, Petrov NA, Samokhvalov EI, Yuferov VP, Vassilenko SK, Uryvaev LV, Kharitonenkov IG, Kaverin NV, Varich NL, Rudneva IA, Gitelman AK (1986) Structural changes in influenza virus hemagglutinin in the process of crossing host range barrier. Dokl Acad Nauk USSR 288: 1002–1005
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kaverin, N.V., Finskaya, N.N., Rudneva, I.A. et al. Studies on the genetic basis of human influenza A virus adaptation to mice: degrees of virulence of reassortants with defined genetic content. Archives of Virology 105, 29–37 (1989). https://doi.org/10.1007/BF01311114
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01311114