Summary
Three cytopathic rotavirus strains were isolated in MA104 cells from intestinal contents of lambs with diarrhea. Pretreatment of virus with pancreatin and incorporation of a small amount of pancreatin in maintenance medium were important for establishment of the strains in these cells. The isolates showed marked cross reactions with bovine rotavirus in immunofluorescence, but no cross reaction with bovine, human, simian, equine, porcine and lapine rotaviruses in neutralization.
References
Fukusho, A., Shimizu, Y., Ito, Y.: Isolation of cytopathic porcine rotavirus in cell roller culture in the presence of trypsin. Arch. Virol.69, 49–60 (1981).
Imagawa, H., Ando, Y., Sugiura, T., Wada, R., Hirasawa, K., Akiyama, Y.: Isolation of foal rotavirus in MA-104 cells. Bull. Equine Res. Inst.18, 119–128 (1981).
Imagawa, H., Wada, R., Hirasawa, K., Akiyama, Y., Oda, I.: Isolation of equine rotavirus in cell cultures from foals with diarrhea. Jpn. J. Vet. Sci.46, 1–9 (1984).
Malherbe, H. H., Striockland-Cholmley, M.: Simian virus S.A.11 and the related O agent. Arch. ges. Virusforsch.22, 235–245 (1967).
McNulty, M. S., Allan, G. M., Pearson, G. R., McFerran, J. B., Curran, W. L., McCracken, R. M.: Reovirus-Like Agent (Rotavirus) from Lambs. Infect. Immun.14, 1332–1338 (1976).
Mebus, C. A., Kono, M., Underdahl, N. R., Twiehous, M. J.: Cell culture propagation of neonated calf diarrhea (scours) virus. Canad. Vet. J.12, 69–72 (1971).
Sato, K., Inaba, Y., Shinozaki, T., Matumoto, M.: Neutralizing antibody to bovine rotavirus in various animal species. Vet. Microbiol.6, 259–261 (1981).
Sato, K., Inaba, Y., Shinozaki, T., Fujii, R., Matumoto, M.: Isolation of human rotavirus in cell cultures. Arch. Virol.69, 155–160 (1981).
Sato, K., Inaba, Y., Miura, Y., Tokuhisa, S., Matumoto, M.: Isolation of lapine rotavirus in cell cultures. Arch. Virol.71, 267–271 (1982).
Snodgrass, D. R., Smith, W., Gray, E. W., Herring, J. A.: A rotavirus in lambs with diarrhoea. Res. Vet. Sci.20, 113–114 (1976).
Snodgrass, D. R., Wells, P. W.: Rotavirus infection in lambs: Studies on passive protection. Arch. Virol.52, 201–205 (1976).
Snodgrass, D. R., Herring, J. A., Linklater, K. A., Dyson, D. A.: A survey of rotaviruses in sheep in Scotland. Vet. Rec.100, 341 (1977).
Takahashi, E., Inaba, Y., Sato, K., Kurogi, H., Akashi, H., Satoda, K., Omori, T.: Antibody to rotavirus in various animal species. Nat. Inst. Anim. Hlth Quart.19, 72–73 (1979).
Theil, K. W., Bhol, E. H., Agnes, A. G.: Cell culture propagation of porcine rotavirus (revoirus-like agent). Am. J. Vet. Res.38, 1765–1768 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Makabe, T., Komaniwa, H., Kishi, Y. et al. Isolation of ovine rotavirus in cell cultures. Archives of Virology 83, 123–127 (1985). https://doi.org/10.1007/BF01310971
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01310971