Skip to main content
SpringerLink
Log in

Organization of polyoma virus DNA in mouse tumor cell lines

  • Original Papers
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

Restriction mapping of polyoma virus DNA in mouse tumor cell lines gave patterns that varied with the cell line examined. These reflected differences in both the organization and the state of integration of virus genomes in the host chromosomes. (The cell lines were derived from tumors induced by polyoma virusin vivo and were propagated continuously in culture.) Two of the cell lines contained multiple copies of tandemly integrated virus genomes as well as free virus DNA molecules. Two other cell lines appeared to contain only integrated virus genomes arranged as tandem repeats. Based on restriction analysis with eleven different endonucleases, the virus DNA in one of the cell lines containing both free and integrated virus genomes was not detectably defective or hypermethylated. This is in contrast to most previously described polyoma virus transformed mouse cells. These virus genomes may, however, contain point mutations or unobserved rearrangements. The second cell line possessing free virus DNA molecules contained both nondefective and defective virus genomes. Most, if not all, defective virus genomes in this line were integrated. The two other cell lines possessing only detectable integrated virus DNA apparently contained only defective virus genomes. The defect in both cases was a small deletion (≦0.2 kb) encompassing 0.12 map units on the physical map of polyoma virus DNA, a region coding for the proximal part of the large T antigen. Moreover, in contrast to the cell lines with free and detectably nondefective virus DNA, the virus DNA was extensively methylated in cell lines containing only integrated and defective virus genomes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Arya, S. K.: Organization of polyoma virus DNA in mammary tumors of athymic mice. J. gen. Virol.63, 405–415 (1982).

    Google Scholar 

  2. Arya, S. K., Galarraga, J. J.: Mouse mammary tumor virus and polyoma virus information in mammary tumours of athymic mice inoculated with polyoma virus. J. gen. Virol.58, 107–114 (1982).

    Google Scholar 

  3. Arya, S. K., Yang, J. T.: Interaction of steroids with nucleic acids. Biochemistry14, 963–969 (1975).

    Google Scholar 

  4. Basilico, C., Gattoni, S., Zouzias, D., Vallee, G. D.: Loss of integrated viral DNA sequences in polyoma virus transformed cells is associated with an active viral A function. Cell.17, 645–659 (1979).

    Google Scholar 

  5. Basilico, C., Zouzias, D., Della Valle, G., Gattoni, S., Colantuoni, V., Fenton, R., Dailey, L.: Integration and excision of polyoma virus genomes. Cold Spring Harbor Symp. Quant. Biol.44, 611–620 (1980).

    Google Scholar 

  6. Benjamin, T. L.: The hr-t gene of polyoma virus. Cold Spring Harbour Symp. Quant. Biol.44, 263–270 (1980).

    Google Scholar 

  7. Bird, A. P., Southern, E. M.: Use of restriction enzymes to study eukaryotic DNA methylation: I. The methylation pattern in ribosomal DNA from Xenopus laevis. J. Mol. Biol.118, 27–28 (1978).

    Google Scholar 

  8. Birg, F., Dulbecco, R., Fried, M., Keman, R.: State and organization of polyoma virus DNA sequences in transformed rat cell lines. J. Virol.29, 633–640 (1979).

    Google Scholar 

  9. Chartrand, P., Gusew-Chartrand, N., Bourgaux, P.: Integrated polyoma genomes in inducible permissive transformed cells. J. Virol.39, 185–195 (1981).

    Google Scholar 

  10. Chowdhury, K., Meltzer, M. L., Israel, M.: Stability of polyoma DNA sequences and virus-coded proteins during tumor formation. J. Virol.41, 1000–1006 (1982).

    Google Scholar 

  11. Cohen, J. C.: Methylation of milk borne and genetically transmitted mouse mammary tumor virus proviral DNA. Cell.19, 653–662 (1980).

    Google Scholar 

  12. Colantuoni, V., Dailey, L., Valle, G. D., Basilico, C.: Requirements for excision and amplification of integrated viral DNA molecules in polyoma virustransformed cells. J. Virol.43, 617–628 (1982).

    Google Scholar 

  13. Dawe, C. J.: Polyoma tumors in mice and X cell tumors in fish, viewed through telescope and microscope (Nakahara Memorial Lecture). In:Dawe, C. J.,et al. (eds.), Phyletic Approaches to Cancer, 19–49. Tokyo: Japanese Scientific Society Press 1981.

    Google Scholar 

  14. Dawe, C. J., Law, L. W., Dunn, T. B.: Studies of parotid tumor agent in cultures of leukemic tissues of mice. J. Nat. Canc. Inst.23, 717–798 (1959).

    Google Scholar 

  15. Desrosiers, R. C., Mulder, C., Fleckenstein, B.: Methylation of herpes-virus saimiri DNA in lymphoid tumor cell lines. Proc. Nat. Acad. Sci. U.S.A.76, 3839–3843 (1979).

    Google Scholar 

  16. DiMayorca, G., Callender, J., Marin, G., Giordano, R.: Temperature-sensitive mutants of polyoma virus. Virology38, 126–133 (1969).

    Google Scholar 

  17. Eckhart, W.: Complementation and transformation by temperature sensitive mutants of polyoma virus. Virology38, 120–125 (1969).

    Google Scholar 

  18. Fried, M.: Cell transforming ability of a temperature-sensitive mutant of polyoma virus. Proc. Nat. Acad. Sci. U.S.A.53, 486–491 (1965).

    Google Scholar 

  19. Griffin, B. E., Soeda, E., Barrell, B. G., Staden, R.: Sequence and analysis of polyoma virus DNA. In:Tooze, J. (ed.), Molecular Biology of Tumor Viruses, 2nd ed., 831–892. New York: Cold Spring Harbor Laboratory 1980.

    Google Scholar 

  20. Harbers, K., Schnieke, A., Stuhlmann, H., Jahner, D., Jaenisch, R.: DNA methylation and gene expression: Endogenous retroviral genome becomes infectious after molecular cloning. Proc. Nat. Acad. Sci. U.S.A.78, 7609–7613 (1981).

    Google Scholar 

  21. Hayday, A. C., Chaudry, F., Fried, M.: Loss of polyoma virus infectivity as a result of a single amino acid change in a region of polyoma virus large T antigen which has extensive amino acid homology with simian virus 40 large T antigen. J. Virol.45, 693–699 (1983).

    Google Scholar 

  22. Ito, Y., Spurr, N.: Polyoma virus T antigens expressed in transformed cells: significance of middle T antigen in transformation. Cold Spring Harbor Symp. Quant. Biol.44, 149–157 (1980).

    Google Scholar 

  23. Lania, L., Hayday, A., Bjursell, G., Gandini-Attardi, D., Fried, M.: Organization and expression of integrated polyoma virus DNA sequences in transformed rodent cells. Cold Spring Harbor Symp. Quant. Biol.44, 597–603 (1980).

    Google Scholar 

  24. McKeon, C., Ohkubo, H., Pastan, I., de Crombrugghe, B.: Unusual methylation pattern of the α2(I) collagen gene. Cell29, 203–210 (1982).

    Google Scholar 

  25. Razin, A., Friedman, J.: DNA methylation and its possible biological role. Prog. Nuc. Ac. Res. Mol. Biol.25, 33–52 (1981).

    Google Scholar 

  26. Rigby, D. W. J., Dieckman, M., Rhodes, C., Berg, P.: Labeling deoxyribonucleic acid to high specific activityin vitro by nick-translation with DNA polymerase I. J. Mol. Biol.113, 237–252 (1977).

    Google Scholar 

  27. Soeda, E., Arrand, J. R., Smolar, N., Walsch, J. E., Griffin, B. E.: Coding potential and regulatory signals of the polyoma virus genome. Nature (London)283, 445–453 (1980).

    Google Scholar 

  28. Southern, E. M.: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol.98, 503–517 (1975).

    Google Scholar 

  29. Sutter, D., Doerfler, W.: Methylation of integrated adenovirus type 12 DNA sequences in transformed cells is inversely correlated with viral gene expression. Proc. Nat. Acad. Sci. U.S.A.77, 253–256 (1980).

    Google Scholar 

  30. Tooze, J. (ed.): Molecular Biology of Tumor Viruses, 2nd ed., 297–337. New York: Cold Spring Harbor Laboratory 1980.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Pathology, National Cancer Institute, National Institutes of Health Bethesda, Maryland, USA

    S. K. Arya

Authors
  1. S. K. Arya
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

With 6 Figures

Rights and permissions

Reprints and permissions

About this article

Cite this article

Arya, S.K. Organization of polyoma virus DNA in mouse tumor cell lines. Archives of Virology 79, 205–219 (1984). https://doi.org/10.1007/BF01310812

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01310812

Keywords

Search

Navigation