Summary
Treatment of purified rubella virus with mixed glycosidases resulted in loss of haemagglutinating (HA) activity but the capacity to combine with haemagglutination inhibiting (HI) and other antibodies was retained. Electrophoretic analysis revealed a greater effect on VPI than VPII.
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Ho-Terry, L., Cohen, A.: Degradation of rubella virus envelope components. Arch. Virol.65, 1–13 (1980).
Ho-Terry, L., Cohen, A.: Effect of 2-mercaptoethanol on the haemagglutinating activity and antigenic properties of rubella virus. Arch. Virol.70, 199–206 (1981).
Liebhaber, H.: Measurement of rubella antibody by haemagglutination inhibition. I. Variables affecting rubella haemagglutination. J. Immunol.104, 818–825 (1970a).
Liebhaber, H.: Measurement of rubella antibody by haemagglutination inhibition. II. Characteristics of an improved HAI test employing a new method for the removal of nonimmunoglobulin HA inhibitor from serum. J. Immunol.104, 826–834 (1970b).
Liebhaber, H., Gross, P. A.: The structural proteins of rubella virus. Virology47, 684–693 (1972).
Maizel, J. V.: Polyacrylamide gel electrophoresis of viral proteins. In:Maramorosch, K., Koprowski, H. (eds.), Methods in Virology, Vol. 5, 179–246. New York-London: Academic Press 1971.
McCarthy, M., Harrison, S. C.: Glycosidase susceptibility: a probe for the distribution of glycoprotein oligosaccharides in sindbis virus. J. Virol.23, 61–73 (1977).
Vaheri, A., Salonen, E.-M.: Evaluation of solid phase enzyme immunoassay procedure in immunity surveys and diagnosis of rubella. J. Med. Virol.5, 171–181 (1980).
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Ho-Terry, L., Cohen, A. The role of glycosylation on haemagglutination and immunological reactivity of rubella virus. Archives of Virology 79, 139–146 (1984). https://doi.org/10.1007/BF01310807
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DOI: https://doi.org/10.1007/BF01310807