Skip to main content
SpringerLink
Log in

Electron microscopy, plaque assay and preliminary serological characterization of three ectromelia virus strains isolated in Poland in the period 1986–1988

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Summary

Three strains of viruses have been identified as ectromedia virus (EV) based on their origin, clinical features, morphology, size of virions (140×220nm), replicative ability and specific cytoplasmatic fluorescence. The mean diameter of plaques produced by EV strains was 0.76mm (range 0.5–1.0mm). The neutralizing properties of the tested sera were evaluated by seroneutralization (SN) and hemagglutination inhibition (HAI) tests.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Allen AM, Clarke GL, Ganaway JR, Lock A, Werner RM (1981) Pathology and diagnosis of mousepox. Lab Anim Sci 31: 599–608

    Google Scholar 

  2. Ardoin P (1983) Virus and diagnostic virology, 1st edn. Maloine SA, Paris, pp 615–808 (in French)

    Google Scholar 

  3. Bhatt PN, Jacoby RO, Gras L (1988) Mousepox in inbred mice innately resistant or susceptible to lethal infection with ectromelia virus. IV. Studies with the Moscow strain. Arch Virol 100: 221–230

    Google Scholar 

  4. Briody BA (1959) Response of mice to ectromelia and vaccinia viruses. Bacteriol Rev 23: 61–95

    Google Scholar 

  5. Buller RM, Bhatt PN, Wallace GD (1983) Evaluation of an enzyme-linked immunosorbent assay for the detection of ectromelia (mousepox) antibody. J Clin Microbiol 18: 1220–1225

    Google Scholar 

  6. Buller RM, Weinblatt AC, Hamburger AW, Wallace GD (1987) Observations in the replication of ectromelia virus in mouse-derived cell lines: implications for epidemiology of mousepox. Lab Anim Sci 37: 28–32

    Google Scholar 

  7. Christensen LR, Weisbroth S, Matanic B (1966) Detection of ectromelia antibodies by immunofluorescence. Lab Anim Care 16: 129–141

    Google Scholar 

  8. Fenner F (1949) Studies in mousepox (infectious ectromelia of mice). VI. A comparison of the virulence and infectivity of three strains of ectromelia virus. Aust J Exp Biol Med Sci 27: 31–43

    Google Scholar 

  9. Fenner F (1981) Mousepox (infectious ectromelia). Past, present and future. Lab Anim Sci 31: 353–359

    Google Scholar 

  10. Jacoby RO, Bhatt PN (1987) Mousepox in inbred mice innately resistant or susceptible to lethal infection with ectromelia virus. II. Pathogenesis. Lab Anim Sci 37: 14–20

    Google Scholar 

  11. Kawamura A Jr, Aoyama Y (1983) Immunofluorescence in medical science, 1st edn. University of Tokyo Press, Springer, Berlin Heidelberg New York, pp 17–30

    Google Scholar 

  12. Malicki K (1967) Pathogenicity of Poxvirus muris strain for 1-day old chicken, embryos and chicken embryos fibroblasts. In: Proceedings of the 16th Congress of the Polish Microbiological Society, Lublin, p 259 (in Polish)

  13. Malicki K (1977) Problems of virus disease diagnosis in laboratory animals. Zwierzeta Lab 14: 9–19 (in Polish)

    Google Scholar 

  14. Malicki K (1977) Mouse smallpox — its epizootiology, pathogenesis and diagnosis. Zwierzeta Lab 14: 21–27 (in Polish)

    Google Scholar 

  15. Manning PJ, Frisk GS (1981) Clinical, pathologic and serologic features of an epizootic of mousepox in Minnesota. Lab Anim Sci 31: 574–577

    Google Scholar 

  16. Marchal J (1930) Infectious ectromelia. A hitherto undescribed virus disease of mice. J Pathol Bacteriol 33: 713–728

    Google Scholar 

  17. Matthews REF (1982) Classification and nomenclature of viruses. Intervirology 17: 42–46

    Google Scholar 

  18. Sadowski W (1974) Laboratory animals for virological studies and their natural viral infections. Post Hig Med Dośw 28: 977–999 (in Polish)

    Google Scholar 

  19. Spohr de Faundez I, Malicka E, Niemialtowski M, Gierynska M, Malicki K (1989) Preliminary characterization of strains of mousepox virus (ectromelia) isolated in Poland. In: Proceedings of the Fifth Virological Symposium of the Polish Microbiological Society. Puławy, pp 58–59

  20. Szynkiewicz E (1965) Ectromelia on a breeding farm for laboratory animals. Zwierzeta Lab 3: 149–163 (in Polish)

    Google Scholar 

  21. Taytsch Z, Wróblewska Z (1956) Epizootic of ectromelia in laboratory mice. Med Dośw Mikrobiol 8: 213 (in Polish)

    Google Scholar 

  22. Wagner JE, Daynes RA (1981) Observations of an outbreak of mousepox in laboratory mice in 1979 at the University of Utah Medical Center U.S.A. Lab Anim Sci 31: 565–569

    Google Scholar 

  23. Wallace GD, Buller RML (1986) Ectromelia virus (mousepox): Biology, epizootiology, prevention and control. In: Bhatt PN, Jacoby RO, Morse HC III, New AE (eds) Viral and mycoplasmal infections of laboratory rodent. Effects on biomedical research. Academic Press, Orlando, pp 539–556

    Google Scholar 

  24. Westwood JCN, Harris WJ, Zwartouw HT, Titmus DHJ, Appleyard G (1964) Studies on the structure of vaccinia virus. J Gen Microbiol 34: 67–78

    Google Scholar 

Download references

Author information

Author notes

  1. M. Niemialtowski

    Present address: Virology and Immunology Laboratory, Department of Microbiology, The University of Tennessee, Knoxville, Tennessee, U.S.A.

Authors and Affiliations

  1. Division of Virology, Department of Microbiology, Warsaw Agricultural University, Warsaw, Poland

    I. Spohr de Faundez, M. Niemialtowski, K. Malicki, M. Gieryńska & A. Popis

  2. Department of Pathology, Faculty of Veterinary Medicine, Warsaw Agricultural University, Warsaw, Poland

    E. Malicka

Authors
  1. I. Spohr de Faundez
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Niemialtowski
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Malicka
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. Malicki
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Gieryńska
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. A. Popis
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Spohr de Faundez, I., Niemialtowski, M., Malicka, E. et al. Electron microscopy, plaque assay and preliminary serological characterization of three ectromelia virus strains isolated in Poland in the period 1986–1988. Archives of Virology 114, 285–291 (1990). https://doi.org/10.1007/BF01310759

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01310759

Keywords

Search

Navigation