Summary
A map of the sites in the genome ofCostelytra zealandica iridescent virus (CzIV), using the restriction enzymesBamHI,KpnI, andPstI, showed the genome size to be 170.2 kbp in length. It was found that the genome was cyclically permuted and that 39% of the genome of CzIV contained repetitive sequence elements. The genome was found to hybridize with the genome of another iridescent virus, type 9 (WIV), in DNA-DNA hybridization experiments. A region of the WIV DNA genome (23.4 kbp) did not hybridize with CzIV DNA and this region is similar in size to the total genomic size difference between CzIV and WIV (22.4 kbp). A unique repeat sequence from iridescent virus type 6 (CIV) was shown to be present in the genome of WIV but not that of CzIV. Finally, the positions of the major capsid protein genes, VP53 and VP52, in the restriction enzyme maps for type 16 and type 9 respectively were determined.
References
Devauchelle G, Stoltz DB, Darcy-Tripper F (1985) Comparative ultrastructure ofIridoviridae. Curr Contents Microbiol Immunol 116: 1–18
Fischer M, Schnitzler P, Delius H, Darai G (1989) Identification and characterization of the repetitive DNA element in the genome of insect iridescent virus type 6. Virology 167: 485–497
Goorha R, Murti KH (1982) The genome of frog virus 3, an animal DNA virus, is circularly permuted and terminally rebundant. Proc Natl Aca Sci USA 79: 248–252
Hibbon JA, Kelly DC (1981) Iridescent virus type 22 DNA. Arch Virol 68: 9–18
Howey PM, Israel MA, Law M, Martin MA (1979) A rapid method for detecting and mapping homology between heterologous DNAs. J Biol Chem 54: 4878–4883
Kalmakoff J, Moore S, Pottinger RP (1972) An iridescent virus from the Grass GrubCostelytra zealandica: serological study. J Invertebr Pathol 20: 70–76
Kalmakoff J, McMillan NAJ, Davison S (1990) Insect iridescent virus type 9 and 16. In: Darai G (ed) Molecular biology of iridoviruses. Kluwer, Boston, pp 113–136
Kalmakoff J, Robertson JS (1970) Serological relationship ofWiseana iridescent virus (WIV) to other iridescent viruses. Proc Univ Otago Med School 48: 16–18
Matsudaira P (1987) Sequencing from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J Biol Chem 262: 10035–10038
Roizman B (1979) The structure and isomerisation of Herpes simplex virus genomes. Cell 16: 481–494
Schnitzler P, Soltau JB, Fischer M, Reisner H, Scholz J, Delius H, Darai G (1987) Molecular cloning and physical mapping of the genome of iridescent virus type 6: further evidence for circular permutation of the viral genome. Virology 160: 66–74
Schnitzler P, Delius H, Scholz J, Touray M, Orth E, Darai G (1987) Identification and nucleotide sequence analysis of the repetitive DNA element in the genome of fish lymphocystis disease virus. Virology 161: 570–578
Stillman BW, Topp WC, Engler JA (1982) Conserved sequences at the origin of adenovirus DNA replication. J Virol 44: 530–537
Tajbakhsh S, Dove MJ, Lee PE, Seligy VL (1986) DNA components of Tipula iridescent virus. Biochem Cell Biol 64: 495
Tajbakhsh S, Lee PE, Watson DC, Seligy VL (1990) Molecular cloning, characterization, and expression of theTipula iridescent virus capsid gene. J Virol 64: 125–136
Tondre L, Tham TN, Mutin PH, Aubertin AM (1988) Molecular cloning and physical and translational mapping of the frog virus 3 genome. Virology 162: 108–117
Tye B, Huberman JA, Botstein D (1974) Non-random circular permutation of phage P22 DNA. J Mol Biol 85: 501–532
Ward VK, Kalmakoff J (1987) Physical mapping of the DNA genome of insect iridescent virus type 9 fromWiseana larvae. Virology 160: 507–510
Wittek R, Moss B (1980) Tandem repeats within the inverted terminal repetition of vaccinia virus DNA. Cell 21: 277–284
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
McMillan, N.A.J., Davison, S. & Kalmakoff, J. Comparison of the genomes of two sympatric iridescent viruses (types 9 and 16). Archives of Virology 114, 277–284 (1990). https://doi.org/10.1007/BF01310758
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01310758