Summary
Thirty eight human sera, seropositive for herpes simplex virus (HSV) and 56 human sera, seronegative for HSV by immunofluorescence and by ELISA, were investigated for reactivity with a series of overlapping synthetic peptides of HSV type 1 glycoprotein D (gD-1). Thirty four out of the 38 human sera positive for HSV reacted with peptides located between residues 300 and 369; the HSV-negative sera reacted with six of the gD-1 peptides, but with none of the peptides within residues 300 to 369.
References
Arnon R, Teicher E, Scheraga HA (1974) Correlation of conformation and biological activity in lysozyme loop homologs. J Mol Biol 90: 403–407
Barany G, Merrifield RB (1980) Solid-phase peptide synthesis. In: Gross E, Meienhofer J (eds) The peptides: analysis, synthesis, biology, vol 2. Academic Press, New York, pp 1–248
Barker WC, Hunt LT, Orcutt BC, George DG, Yeh LS, Chen HR, Blomquist MC, Johnson GC, Seibel-Ross EI, Ledley RS (1989) Atlas of protein sequence and structure — protein sequence database, version 1989. National Biomedical Research Foundation Washington, DC
Bosch DL, Geerligs HJ, Weijer WJ, Feijlbrief M, Welling GW, Welling-Wester S (1987) Structural properties and reactivity of N-terminal synthetic peptides of herpes simplex virus type 1 glycoprotein D by using anti-peptide antibodies and group VII monoclonal antibodies. J Virol 61: 3607–3611
Cohen GH, Dietzschold B, Ponce de Leon M, Long D, Golub E, Varrichio A, Pereira L, Eisenberg RJ (1984) Localization and synthesis of an antigenic determinant of herpes simplex glycoprotein D that stimulates production of neutralizing antibody. J Virol 49: 102–108
Dayhoff MO, Barker WC, Hunt LT (1983) Establishing homologies in protein sequences. Methods Enzymol 91: 524–545
Eing RB, Kuhn JE, Braun RW (1989) Neutralizing activity of antibodies against the major herpes simplex virus type 1 glycoproteins. J Med Virol 27: 59–65
Eisenberg RJ, Long D, Ponce de Leon M, Matthews JT, Spear PG, Gibson MG, Lasky LA, Golub E, Cohen GH (1985) Localization of epitopes of herpes simplex virus type 1 glycoprotein D. J Virol 53: 634–644
Geerligs HJ, Weijer WJ, Bloemhoff W, Welling GW, Welling-Wester S (1988) The influence of pH and ionic strength on the coating of peptides of herpes simplex virus type 1 in an enzyme-linked immunosorbent assay. J Immunol Methods 106: 239–244
Houghten A (1985) General method for the rapid solid phase synthesis of large numbers of peptides: specificity of antigen-antibody interaction at the level of individual amino acids. Proc Natl Acad Sci USA 82: 5131–5135
Isola VJ, Eisenberg RJ, Siebert GH, Heilman CJ, Wilcox WC, Cohen GH (1989) Fine mapping of antigenic site II of herpes simplex virus glycoprotein D. J Virol 63: 2325–2334
Jacob CO, Sela M, Arnon R (1983) Antibodies against synthetic peptides of the B subunit of cholera toxin. Proc Natl Acad Sci USA 80: 7611–7615
Kuhn JE, Kler G, Munk K, Braun R (1987) Analysis of the IgM and IgG antibody response against herpes simplex virus type 1 (HSV-1) structural and nonstructural proteins. J Med Virol 23: 135–140
Minson AC, Hodgman TC, Digard P, Hancock DC, Bell SE, Buckmaster EA (1986) An analysis of the biological properties of monoclonal antibodies against glycoprotein D of herpes simplex virus and identification of amino acid substitutions that confer resistance to neutralization. J Gen Virol 67: 1001–1013
Strynadka NCJ, Redmond MJ, Parker JMR, Scraba DG, Hodges RS (1988) Use of synthetic peptides to map the antigenic determinants of glycoprotein D of herpes simplex virus. J Virol 62: 3474–3483
Watson RJ, Weis JH, Salstrom JS, Enquist LW (1982) Herpes simplex virus type 1 glycoprotein D gene: nucleotide sequence and expression inEscherichia coli. Science 218: 381–383
Weijer WJ, Drijfhout JW, Geerligs HJ, Bloemhoff W, Feijlbrief M, Bos CA, Hoogerhout P, Kerling KET, Popken-Boer T, Slopsema K, Wilterdink JB, Welling GW, Welling-Wester S (1988) Antibodies against synthetic peptides of herpes simplex virus type 1 glycoprotein D and their capability to neutralize viral infectivity in vitro. J Virol 62: 501–510
Wootton JC, Taylor JG, Jackson AA, Chambers GK, Fincham JRS (1975) The amino acid sequence ofNeurospora NADP-specific glutamate dehydrogenase. Biochem J 149: 739–748
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Geerligs, H.J., Feijlbrief, M., Bolk, M. et al. Reactivity of human sera with overlapping synthetic peptides of herpes simplex virus type 1 glycoprotein D. Archives of Virology 114, 251–258 (1990). https://doi.org/10.1007/BF01310754
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DOI: https://doi.org/10.1007/BF01310754