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Comparative studies ontat mutants of three primate lentiviruses

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Summary

A frame-shifttat gene mutant of human immunodeficiency virus type 1 (HIV-1), which showed no detectable trans-activation potential, was constructed in vitro. Upon transfection, this clone directed the synthesis of virus mRNAs,gag proteins and virion-associated reverse transcriptase (RT) at a low level as was observed with thetat mutants of HIV-2 and simian immunodeficiency virus isolated from African green monkey (SIVAGM). Using these mutant viruses, trans-activation efficiency of viral gene expression bytat was compared among HIV-1, HIV-2, and SIVAGM. SIVAGM seemed to be less dependent ontat for RT production than HIV-1 and HIV-2.

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Authors and Affiliations

  1. Laboratory of Gene Analysis, Department of Viral Oncology, Institute for Virus Research, Kyoto University, Kyoto, Japan

    R. Shibata, H. Sakai, K. Ogawa, A. Ishimoto & A. Adachi

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  1. R. Shibata
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  2. H. Sakai
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  3. K. Ogawa
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  4. A. Ishimoto
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  5. A. Adachi
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Shibata, R., Sakai, H., Ogawa, K. et al. Comparative studies ontat mutants of three primate lentiviruses. Archives of Virology 114, 243–250 (1990). https://doi.org/10.1007/BF01310753

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  • DOI: https://doi.org/10.1007/BF01310753

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